Detection of Meloidogyne enterolobii in potatoes in South Africa and phylogenetic analysis based on intergenic region and the mitochondrial DNA sequences

Onkendi, Edward Makori; Moleleki, Lucy N.

doi:10.1007/s10658-012-0142-y

Cited by 37 publications

(16 citation statements)

References 16 publications

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“…The number of subrepeats is inappropriate for discrimination of Pyropia species, due to the difference within individuals of the same species. The IGS region is more variable than the ITS region; therefore, it is used for phylogenetic analysis in interspecies and intraspecies (Li et al 2010;Onkendi and Moleleki 2013). This is the first time that the complete nuclear ribosomal DNA cistron sequence has been obtained for P. yezoensis.…”

Section: Discussionmentioning

confidence: 99%

The complete nuclear ribosomal DNA (nrDNA) cistron sequence of Pyropia yezoensis (Bangiales, Rhodophyta)

et al. 2015

J Appl Phycol

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The complete nuclear ribosomal DNA (nrDNA) cistron sequence of Pyropia yezoensis has been sequenced, of which each unit is composed of the intergenic spacer (IGS), the small-subunit (SSU) ribosomal RNA (rRNA) gene, the internal transcribed spacer 1 (ITS1), the 5.8S rRNA gene, the internal transcribed spacer 2 (ITS2), and the large-subunit (LSU) rRNA gene. This cistron occurs in tandem repeats that can number in 100s to 1000s. Furthermore, in the analysis of the sequence data, we determined that introns were present in the small-subunit rRNA genes, for which the exon sequences (1834 bp) are almost identical. The intron numbers ranging from zero to two in the small-subunit rRNA genes are different between multiple individuals. However, the full length of the large-subunit rRNA genes is 4770 bp without any introns, and the 5.8S rRNA gene is 159 bp. Moreover, the 5′ and 3′ terminal sequences of the genic regions of the ribosomal cistron are extremely conservative even among various species in red alga. In addition, the range for ITS1 is 371-372 bp and for ITS2 is 532 to 535 bp. The IGS is 5984 bp in length and possesses upstream direct repeats, downstream repeats, and middle inverted repeats that make sequencing untoward. This is the first time that the complete nucleotide of the IGS region of P. yezoensis has been sequenced. The provision of the full length of nrDNA cistron will offer useful information to be widely utilized for identification, taxonomic relationships, and phylogenies in red algae.

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Section: Discussionmentioning

confidence: 99%

The complete nuclear ribosomal DNA (nrDNA) cistron sequence of Pyropia yezoensis (Bangiales, Rhodophyta)

et al. 2015

J Appl Phycol

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“…Three of the five emerging species, M. chitwoodi , M. enterolobii and M. fallax , are also reported from Africa (Table ). For example, the resistance‐breaking apomictic species M. enterolobii has been isolated from Burkina Faso, the Democratic Republic of Congo, Malawi, Mozambique, Senegal, South Africa and Togo, causing damage in potato and guava (M. Marais, unpublished data; Onkendi & Moleleki, ). Meloidogyne chitwoodi and M. fallax have been reported to cause damage of various crop plants in South Africa and Mozambique.…”

Section: Meloidogyne Species Present In Africamentioning

confidence: 99%

“…Can new diagnostic tools based on molecular technology be employed together with classical methods to carry out accurate identification? For instance, Onkendi & Moleleki (,b) have recently demonstrated the use of molecular approaches in accurately identifying various Meloidogyne spp. present in potatoes from South Africa.…”

Section: Meloidogyne Species Present In Africamentioning

confidence: 99%

The threat of root‐knot nematodes (Meloidogyne spp.) in Africa: a review

et al. 2014

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Meloidogyne species pose a significant threat to crop production in Africa due to the losses they cause in a wide range of agricultural crops. The direct and indirect damage caused by various Meloidogyne species results in delayed maturity, toppling, reduced yields and quality of crop produce, high costs of production and therefore loss of income. In addition, emergence of resistance-breaking Meloidogyne species has partly rendered various pest management programmes already in place ineffective, therefore putting food security of the continent at risk. It is likely that more losses may be experienced in the future due to the on-going withdrawal of nematicides. To adequately address the threat of Meloidogyne species in Africa, an accurate assessment and understanding of the species present, genetic diversity, population structure, parasitism mechanisms and how each of these factors contribute to the overall threat posed by Meloidogyne species is important. Thus, the ability to accurately characterize and identify Meloidogyne species is crucial if the threat of Meloidogyne species to crop production in Africa is to be effectively tackled. This review discusses the use of traditional versus molecular-based identification methods of Meloidogyne species and how accurate identification using a polyphasic approach can negate the eminent threat of root knot nematodes in crop production. The potential threat to Africa posed by highly damaging and resistance-breaking populations of 'emerging' Meloidogyne species is also examined.

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“…It attacks herbaceous as well as woody plants. The principal hosts are Phaseolus vulgaris (bean), Coffea arabica (coffee), Gossypium hirsutum L. (cotton), Solanum melongena (eggplant), Psidium guajava (guava), Solanum quitoense (naranjilla), Carica papaya L. (papaya), Capsicum annuum (pepper), Solanum tuberosum (potato), Glycine max (soybean), Ipomoea batatas (sweet potato), Nicotiana tabacum (tobacco), Lycopersicon esculentum (tomato) and Citrullis lanatus (watermelon) (Rammah & Hirschmann, ; Brito et al ., ; Gomes et al ., ; Bitencourt & Silva, ; Silva et al ., ; Quénéhervé et al ., ; Crozzoli et al ., ; da Silva & Krasuski, ; Onkendi & Moleleki, ; Ye et al ., ). For Gossypium hirsutum (cotton), Brito et al .…”

Section: Hostsmentioning

confidence: 99%

Meloidogyne enterolobii

2014

EPPO Bulletin

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Detection of Meloidogyne enterolobii in potatoes in South Africa and phylogenetic analysis based on intergenic region and the mitochondrial DNA sequences

Cited by 37 publications

References 16 publications

The complete nuclear ribosomal DNA (nrDNA) cistron sequence of Pyropia yezoensis (Bangiales, Rhodophyta)

The complete nuclear ribosomal DNA (nrDNA) cistron sequence of Pyropia yezoensis (Bangiales, Rhodophyta)

The threat of root‐knot nematodes (Meloidogyne spp.) in Africa: a review

Meloidogyne enterolobii

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