Detection of melanoma cells in vitro using an optical detector of photoacoustic waves

Gutiérrez-Juárez, G.; Gupta, Sagar K.; Al-Shaer, Mays; Polo‐Parada, Luis; Dale, Paul S.; Papageorgio, Chris; Viator, John A.

doi:10.1002/lsm.20894

Cited by 21 publications

(18 citation statements)

References 19 publications

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“…5 Photoacoustic flow cytometry (PAFC) has been used both in vivo and in vitro to detect circulating melanoma cells, which are naturally optically absorbing. 6,7 Most other cancers are nonpigmented and inaccessible to PAFC without cellular alteration. Viator et al 8 demonstrated the ability to add gold nanoparticles (AuNPs) to prostate cancer cells through immunochemistry and detect them using the in vitro cytometry method.…”

Section: Introductionmentioning

confidence: 99%

Detection and capture of breast cancer cells with photoacoustic flow cytometry

2016

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Abstract. According to the Centers for Disease Control and Prevention, breast cancer is the most common cancer and the second leading cause of cancer related deaths among women. Metastasis-the presence of secondary tumors caused by the spread of cancer cells via the circulatory or lymphatic systems-significantly worsens the prognosis of any breast cancer patient. A technique is developed to detect circulating breast cancer cells in human blood using a photoacoustic flow cytometry method. A Q-switched laser is used to interrogate thousands of blood cells with one pulse as they flow through the beam path. Cells that are optically absorbing, either naturally or artificially, emit an ultrasound wave as a result of the photoacoustic (PA) effect. Breast cancer cells are targeted with chromophores through immunochemistry in order to enhance optical absorption. After which, the PA cytometry device is calibrated to demonstrate the ability to detect single cells. Cultured breast cancer cells are added to whole blood to reach a biologically relevant concentration of about 25 to 45 breast cancer cells per 1 mL of blood. An in vitro PA flow cytometer is used to detect and isolate these cells followed by capture with the use of a micromanipulator. This method can not only be used to determine the disease state of the patient and the response to therapy but also it can be used for genetic testing and in vitro drug trials since the circulating cell can be captured and studied.

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Section: Introductionmentioning

confidence: 99%

Detection and capture of breast cancer cells with photoacoustic flow cytometry

2016

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“…Several methods have been developed to detect CTCs in blood samples, including flow cytometry , polymerase chain reaction (PCR) techniques , optical based imaging systems , size and/or density differences , and photoacoustic detection . These methods can be incorporated into microfluidic devices for in vitro measurements .…”

Section: Introductionmentioning

confidence: 99%

Classification of blood cells and tumor cells using label‐free ultrasound and photoacoustics

Strohm

Kolios

2015

Cytometry Pt A

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A label-free method that can identify cells in a blood sample using high frequency photoacoustic and ultrasound signals is demonstrated. When the wavelength of the ultrasound or photoacoustic wave is similar to the size of a single cell (frequencies of 100-500 MHz), unique periodic features occur within the ultrasound and photoacoustic power spectrum that depend on the cell size, structure, and morphology. These spectral features can be used to identify different cell types present in blood, such as red blood cells (RBCs), white blood cells (WBCs), and circulating tumor cells. Circulating melanoma cells are ideal for photoacoustic detection due to their endogenous optical absorption properties. Using a 532 nm pulsed laser and a 375 MHz transducer, the ultrasound and photoacoustic signals from RBCs, WBCs, and melanoma cells were individually measured in an acoustic microscope to examine how the signals change between cell types. A photoacoustic and ultrasound signal was detected from RBCs and melanoma cells; only an ultrasound signal was detected from WBCs. The different cell types were distinctly separated using the ultrasound and photoacoustic signal amplitude and power spectral periodicity. The size of each cell was also estimated from the spectral periodicity. For the first time, sound waves generated using pulse-echo ultrasound and photoacoustics have been used to identify and size single cells, with applications toward counting and identifying cells, including circulating melanoma cells. V C 2015 International Society for Advancement of Cytometry

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“…In recent research, it has been shown that, using an optical detector for the PA waves, it is possible to detect one melanoma cell suspended in saline [11]. In this work, a variant of the PA wave detection is proposed.…”

Section: Introductionmentioning

confidence: 99%

Optical Photoacoustic Detection of Circulating Melanoma Cells In Vitro

et al. 2010

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The purpose of this research was to investigate the sensitivity of a system for the detection of circulating melanoma cells based on the thermoelastic properties of melanoma. The method employs photoacoustic (PA) excitation coupled with an optical transducer capable of determining the presence of cells within the circulating system in vitro. The transducer is based on stress waveinduced changes of the optical reflectance of a glass-water interface, probed with a continuous laser beam that is incident at an angle close to the critical angle of total internal reflection. A frequency tripled Nd:YAG laser pumping an optical parametric oscillator was employed to provide 532 nm and 620 nm laser light with a pulse duration of 10 ns. A custom-made flow chamber was used as an excitation and acoustic wave collection device. The targets were a human melanoma cell line HS 936 with an average diameter of about 15 μm. Melanoma cells were suspended in 10 mL of two types of media. The first one was Tyrode's buffer in concentrations ranging from 10 to 50 cells per μL, and the second one included 10 6 healthy white blood cells per mL of Tyrode's buffer. PA pressure waves were detected by an optical stress transducer. Detection trials resulted in a detection threshold of the order of one individual cell, indicating the effectiveness of the proposed mechanism. Results imply the potential to assay simple blood Correspondence to: J. A. Viator, viatorj@missouri.edu. NIH Public Access

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Detection of melanoma cells in vitro using an optical detector of photoacoustic waves

Abstract: The optical reflectance method provides sensitivity to detect small numbers of melanoma cells without created false-positive signals from pyroelectric interference, showing promise as a means to perform tests for circulating melanoma cells in blood samples.

Cited by 21 publications

References 19 publications

Detection and capture of breast cancer cells with photoacoustic flow cytometry

Detection and capture of breast cancer cells with photoacoustic flow cytometry

Classification of blood cells and tumor cells using label‐free ultrasound and photoacoustics

Optical Photoacoustic Detection of Circulating Melanoma Cells In Vitro

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