Detection of melamine in liquid milk using surface‐enhanced Raman scattering spectroscopy

Zhang, Xiaofang; Zou, Mingqiang; Qi, Xiaohua; Liu, Feng; Zhu, Xinhua; Zhao, B. Y.

doi:10.1002/jrs.2629

Cited by 143 publications

(71 citation statements)

References 34 publications

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“…On the contrary, SERS could give more information than LC-MS. In addition, LC-MS require access to complicated and expensive laboratory facilities, especially in terms of sample preparation and clean-up steps [37]. Finally, the time needed for analyzing each spot on the TLC plate by SERS may be 5∼10 s, while LC-MS requires a minimum of 10 min.…”

Section: Analysis Of the Common And Characteristic Peaks Of Ephedrinementioning

confidence: 99%

Rapid on-site detection of ephedrine and its analogues used as adulterants in slimming dietary supplements by TLC-SERS

Cao

Lou

et al. 2014

Anal Bioanal Chem

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Ephedrine and its analogues are in the list of prohibited substance in adulteration to botanical dietary supplements (BDS) for their uncontrollable stimulating side effects. However, they were always adulterated illegally in BDS to promote losing weight. In order to avoid detection, various kinds of ephedrine analogues were added rather than ephedrine itself. This has brought about great difficulties in authentication of BDS. In this study, we put forward for the first time a method which combined thin-layer chromatography (TLC) and surface-enhanced Raman scattering (SERS) to directly identify trace adulterant. Ephedrine, pseudoephedrine, methylephedrine, and norephedrine were mixed and used in this method to develop an analytical model. As a result, the four analogues were separated efficiently in TLC analysis, and trace-components and low-background SERS detection was realized. The limit of detection (LOD) of the four analogues was 0.01 mg/mL. Eight common Raman peaks (△υ = 620, 1003, 1030, 1159, 1181, 1205, 1454, 1603 cm(-1)) were extracted experimentally and statistically to characterize the common feature of ephedrine analogues. A TLC-SERS method coupled with common-peak model was adopted to examine nine practical samples, two of which were found to be adulterated with ephedrine analogues. Identification results were then confirmed by UPLC-QTOF/MS analysis. The proposed method was simple, rapid, and accurate and can also be employed to trace adulterant identification even when there are no available reference derivatives on-site or unknown types of ephedrine analogues are adulterated.

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Section: Analysis Of the Common And Characteristic Peaks Of Ephedrinementioning

confidence: 99%

Rapid on-site detection of ephedrine and its analogues used as adulterants in slimming dietary supplements by TLC-SERS

Cao

Lou

et al. 2014

Anal Bioanal Chem

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“…The enhancement associated with SERSup to 10 14 times [23]-is widely attributed to two primary mechanisms: the short-range chemical effect and the longrange classical electromagnetic effect. Recently, several groups have reported the determination of melamine using SERS by detecting the signal from melamine directly with roughened gold substrates [24], silver colloid substrate [25] or other SERS substrates [26][27][28]. However, the signal from melamine itself is relatively weak, and the detection limits of these direct sensing methods are unsatisfactory (most are more than 10 ppb).…”

Section: Introductionmentioning

confidence: 99%

Rapid detection of melamine with 4-mercaptopyridine-modified gold nanoparticles by surface-enhanced Raman scattering

et al. 2011

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A surface-enhanced Raman scattering (SERS) strategy based on 4-mercaptopyridine (MPY)-modified gold nanoparticles (AuNPs) was developed for the rapid and sensitive detection of melamine in milk powder. The SERS measurement of melamine strongly relied on the "hotspot" effect, in which AuNPs immediately aggregated upon the addition of melamine, leading to significantly enhanced Raman intensity of the reporter molecule MPY and a color change for the solution from red to blue-gray. The limit of detection based on a signal to noise of 3 (S/N=3) was found to be as low as 0.1 ppb of melamine, with an excellent linearity of 0.5-100 ppb, demonstrating a higher sensitivity and a wider quantitation range than direct SERS sensing methods based on enhanced substrate. An impressive specificity for melamine detection over various common metal ions and excipients in dairy products, even at concentrations of 100-fold higher than melamine, was achieved. Good recoveries of 88.5% and 111.7% were obtained from milk samples spiked to 20 and 100 ppb levels, respectively. The proposed method is potentially applicable for the rapid in situ determination of melamine in complex matrices.

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“…Adulterants can be detected using SERS [180] in wheat gluten, chicken feed, cakes, and noodles [181]. Melamine can be found in liquid milk [182,183], milk powder [184], and infant formula [185] at very low levels.…”

Section: Sers Of Small Moleculesmentioning

confidence: 99%

Fundamentals and applications of SERS-based bioanalytical sensing

et al. 2017

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Abstract:Plasmonics is an emerging field that examines the interaction between light and metallic nanostructures at the metal-dielectric interface. Surface-enhanced Raman scattering (SERS) is a powerful analytical technique that uses plasmonics to obtain detailed chemical information of molecules or molecular assemblies adsorbed or attached to nanostructured metallic surfaces. For bioanalytical applications, these surfaces are engineered to optimize for high enhancement factors and molecular specificity. In this review we focus on the fabrication of SERS substrates and their use for bioanalytical applications. We review the fundamental mechanisms of SERS and parameters governing SERS enhancement. We also discuss developments in the field of novel SERS substrates. This includes the use of different materials, sizes, shapes, and architectures to achieve high sensitivity and specificity as well as tunability or flexibility. Different fundamental approaches are discussed, such as label-free and functional assays. In addition, we highlight recent relevant advances for bioanalytical SERS applied to small molecules, proteins, DNA, and biologically relevant nanoparticles. Subsequently, we discuss the importance of data analysis and signal detection schemes to achieve smaller instruments with low cost for SERS-based point-of-care technology developments. Finally, we review the main advantages and challenges of SERS-based biosensing and provide a brief outlook.

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Detection of melamine in liquid milk using surface‐enhanced Raman scattering spectroscopy

Cited by 143 publications

References 34 publications

Rapid on-site detection of ephedrine and its analogues used as adulterants in slimming dietary supplements by TLC-SERS

Rapid on-site detection of ephedrine and its analogues used as adulterants in slimming dietary supplements by TLC-SERS

Rapid detection of melamine with 4-mercaptopyridine-modified gold nanoparticles by surface-enhanced Raman scattering

Fundamentals and applications of SERS-based bioanalytical sensing

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