Detection of mage‐4 protein in lung cancers

Shichijo, Shigeki; Hayashi, Akira; Takamori, Shinzo; Tsunosue, Rika; Hoshino, Tomoaki; Sakata, Motoko; Kuramoto, Terukazu; Ōizumi, Kōtaro; Itoh, Kyogo

doi:10.1002/ijc.2910640303

Cited by 46 publications

(21 citation statements)

References 13 publications

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“…Members of the MAGE gene family have been identified to have higher expression levels in tumors with relatively larger diameters (23). In this study, we discovered that MAGED4 expression was not significantly correlated with tumor size (P>0.05).…”

Section: Discussionmentioning

confidence: 62%

The significance of MAGED4 expression in non-small cell lung cancer as analyzed by real-time fluorescence quantitative PCR

Pang

Chen

et al. 2012

Oncology Letters

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Abstract. The aim of this study was to detect differences in the expression levels of melanoma-associated antigen D4 (MAGED4) mRNA between non-small cell lung cancer (NSCLC) tissues and normal tissues, and to compare differences in the expression levels of MAGED4 in tumor patients. Patients were grouped according to age, gender, smoking history, tumor size, pathological classification, degree of lung cancer cell differentiation and presence of lymph node metastasis. The expression levels of MAGED4 were detected using real-time fluorescence quantitative PCR. MAGED4 expression was higher in squamous cell carcinomas compared to adenocarcinomas (P<0.05), in poorly differentiated tissues compared to well-differentiated tissues (P<0.05), and in patients with lymph node metastasis compared to patients without lymph node metastasis (P<0.05). MAGED4 may be used as a specific antigen for NSCLC to influence the improvement of diagnosis, prognosis and immunological therapy outcomes in lung cancer patients.

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Section: Discussionmentioning

confidence: 62%

The significance of MAGED4 expression in non-small cell lung cancer as analyzed by real-time fluorescence quantitative PCR

Pang

Chen

et al. 2012

Oncology Letters

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“…Summarized results are shown in Table I. (Van derBruggen et al, 1991, 1994aShichijo et al, 1995b) October 3,1995.…”

Section: Mage-1 Gene Is Expressed In T-cell Leukemiamentioning

confidence: 99%

MAGE-1 gene is expressed in T-cell leukemia

Shichijo¹,

Sagawa²,

Brasseur³

et al. 1996

Int. J. Cancer

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“…Deregulated expression of GAGE or MAGE melanoma-derived tumor antigen family members in cancer has been documented in melanoma, lung, breast, bladder, stomach, and tumors of neuroectodermal origin (25, 29 -35) among others with some showing promise for predicting disease progression (32,34). The lack of PAGE-1 expression in MCF-7 breast cancer cells is similar to that described previously for MAGE (25,36), while other breast cancer cell lines were positive for MAGE expression.…”

Section: Fig 4-continuedmentioning

confidence: 99%

Isolation and Characterization of PAGE-1 andGAGE-7

Chen¹,

Lin²,

Chung³

et al. 1998

Journal of Biological Chemistry

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The LNCaP progression model of human prostate cancer consists of lineage-related sublines that differ in their androgen sensitivity and metastatic potential. A differential display polymerase chain reaction was employed to evaluate mRNA expression differences between the LNCaP sublines in order to define the differences in gene expression between the androgensensitive, nontumorigenic LNCaP cell line and the androgen-insensitive, metastatic LNCaP sublines, C4-2 and C4-2B. An amplicon, BG16.21, was isolated that showed increased expression in the androgen-independent and metastatic LNCaP sublines, C4-2 and C4-2B. Hybridization screening of a gt11 expression library with BG16.21 revealed two transcripts, both homologous to BG16.21 at the 3 end. A GenBank TM data base search using the GCG Wisconsin software package revealed the shorter ϳ600-bp transcript (designated GAGE-7) to be a new member of the GAGE family. The second ϳ700-bp transcript was a novel gene (designated PAGE-1, "prostate associated gene") with only 45% homology to GAGE gene family members. RNA blot analysis demonstrated that GAGE-7 mRNA was expressed at equal levels in all lineage related prostate cancer cell sublines, while PAGE-1 mRNA levels were elevated 5-fold in C4-2 and C4-2B as compared with LNCaP cells. Neither GAGE-7 nor PAGE-1 demonstrated any regulation by androgens in the prostate cancer cell lines used in this study. PAGE-1 and GAGE-7 expression was found to be restricted to testes (high) and placenta (low) on human multiple tissue Northern blots. As GAGE/MAGE antigens were reported previously to be targets for tumor-specific cytotoxic lymphocytes in melanoma, these results suggest that PAGE-1 and GAGE-7 may be related to prostate cancer progression and may serve as potential targets for novel therapies.An experimental model system to study advanced and metastatic prostate cancer has been developed based on the observation that nontumorigenic bone or prostate fibroblasts, when co-inoculated with the nontumorigenic, prostate-specific antigen (PSA) 1 -secreting cell line LNCaP, induced prostate adenocarcinoma growth in vivo in nude mice (1, 2). By manipulating the androgen status of the hosts, the laboratory was able to generate various LNCaP sublines from the primary tumors (3, 4). The direct lineage-related sublines C4, C4 -2, and C4 -2B4 share cytogenetic markers and HLA haplotype with parental LNCaP, but otherwise possess distinct biologic and biochemical profiles. Most importantly, the sublines exhibit progressive tumorigenicity, metastatic potential, and androgen independence. C4, the first subline derived from LNCaP, forms tumors in intact nude mice without the need for a co-inoculum of bone fibroblasts or Matrigel®; while growth in castrated hosts still requires a co-inducer. C4-2 is a subline derived from C4, which is highly tumorigenic in intact or castrated nude mice without a co-inducer. C4-2B4 is a bone metastatic subline derived from orthotopic injection of C4-2 and has demonstrated an increased aggressiveness as compared wi...

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Detection of mage‐4 protein in lung cancers

Cited by 46 publications

References 13 publications

The significance of MAGED4 expression in non-small cell lung cancer as analyzed by real-time fluorescence quantitative PCR

The significance of MAGED4 expression in non-small cell lung cancer as analyzed by real-time fluorescence quantitative PCR

MAGE-1 gene is expressed in T-cell leukemia

Isolation and Characterization of PAGE-1 andGAGE-7

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