Detection of Macromolecular Content in a Mixed Solution of Protein Macromolecules and Small Molecules Using a Weak Measurement Linear Differential System

Abstract

In this paper, we propose a linear differential detection system based on a frequency domain weak measurement. The system can be used for detecting optical substances. Moreover, we completed an experiment to detect himan serum albumin (HSA) content in a mixture of human serum albumin and L-proline via dialysis. This work also proves the differential function of the system. This experiment can be further extended to detecting protein content in a mixed solution that contains protein macromolecules and various small molecules. It is very important for detecting molecules without photomarking in solutions of complex biological samples. In this paper, the system has an optical resolution of 1.39 × 10 −5 , and resolution of 4.06 × 10 −8 mol/L for himan serum protein solution.

Optimization of the Weak Measurement System by Determining the Optimal Total Phase Difference

“…Considering that under the premise of satisfying the weak value amplification, it is θ α 1 [10], [20], [21], and I ≈ sin 2 α. By adjusting the phase difference of the QWP, the light intensity of the system can be adjusted.…”

“…Furthermore, we also combined the weak measurement with a polarization detection system to detect the chiral molecule content by using optical rotation. This led to applications such as a chiral sensor based on weak measurement [9], detection of macromolecular content in a mixed solution [10]and detection of amino acid concentration [11]. Application of the weak measurement technology in the above-mentioned two types of systems has increased the detection sensitivity of the original systems by three and two orders of magnitude respectively [12].…”

Oxidation Analysis of l-Cysteine with a Chiral Sensor Based on Quantum Weak Measurement

“…Furthermore, they also utilized quantum weak measurement to monitor the real-time hydrolysis process of sucrose in 2021 . The optical rotation sensor has the capability to detect amino acids and proteins . Some previous studies on glucose or chiral optical sensors based on weak measurement have demonstrated encouraging outcomes. − In this paper, the application of weak measurement in the real-time determination of l -cysteine is expanded, which is of great significance for studying the kinetic reaction process of l -cysteine.…”

Demonstration of a New Characterization Method for Weak Measurement

“…Since 2010, relevant theories have shown that the weak measurement technology exhibits more obvious detection advantages in the frequency domain than the other fields ( Brunner and Simon, 2010 ; Xu et al, 2013 ). In 2015, our group first proposed a novel optical frequency domain weak measurement system with universal value, which has shown amazing potential in the field of biomolecular detection ( Zhang et al, 2016 ; Li et al, 2017 ; Li et al, 2018 ; Xu et al, 2018 ; Xu et al, 2019a ; Xu et al, 2019b ; Xu et al, 2021 ).…”