Detection of Lung Cancer Using Plasma Protein Profiling by Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry

Abstract

There are no satisfactory plasma biomarkers which are available for the early detection and monitoring of lung cancer, one of the most frequent cancers worldwide. The aim of this study is to explore the application of matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-ToF MS) to plasma proteomic patterns to distinguish lung cancer patients from healthy individuals. The EDTA plasma samples have been pre-fractionated using magnetic bead kits functionalized with weak cation exchange coatings. We compiled MS protein profiles for 90 patients with squamous cell carcinomas (SCC) and compared them with profiles from 187 healthy controls. The MALDI-ToF spectra were analyzed statistically using ClinProTools bioinformatics software. Depending on the sample used, up to 441 peaks/spectrum could be detected in a mass range of 1000-20,000 Da; 33 of these proteins had statistically differential expression levels between SCC and control plasma (P < 0.001). The series of the peaks were automatically chosen as potential biomarker patterns in the training set. They allowed the discrimination of plasma samples from healthy control and samples from SCC patients (sensitivity and specificity >90%) in external validation test. These results suggest that plasma MALDI-ToF MS protein profiling can distinguish patients with SCC and also from healthy individuals with relatively high sensitivity and specificity and that MALDI- ToF MS is a potential tool for the screening of lung cancer.

Clinical Application of Mass Spectrometry‐Based Proteomics in Lung Cancer Early Diagnosis

“…Sample fractionation can be achieved through different approaches, which include gel electrophoresis (see Table 2), isolectrofocusing or anionic/cationic exchange fractionation protocols. [23,28] One of the most widely used approaches, which has been employed in 18 of the studies analyzed, is represented by protein separation by 2D-GE, followed by MS identification of differential spots individually excised from the gel. In 2D-GE, proteins are separated orthogonally on two dimensions, based on their isoelectric point and molecular mass.…”

Strain differentiation of staphylococcus aureus by means of direct maldi tof mass spectrometry profiling

“…Программное обеспечение ClinProTools 2.0, как подход к групповой кластеризации масс-спектров, создавалось для поиска маркеров белковой природы к различным онкологическим заболеваниям [22][23][24].…”

Search of potential gastric cancer biomarkers using low molecular weight blood plasma proteome profiling by mass spectrometry

“…С помощью времяпролетной масс-спектрометрии с матрично-активированной лазерной десорбцией/ионизацией (MALDI-TOF-MS) можно получить профили экспрессии белков в биологических образцах человека и обнаружить потенциальные биомаркеры рака. Недавно показана эффективность этой технологии для открытия белковых маркеров в сыворотке/плазме крови человека [7][8][9][10][11].…”