Detection of low-populated reaction intermediates with hyperpolarized NMR

Abstract

Hyperpolarized (13)C NMR spectroscopy can provide the sensitivity and spectral resolution to detect, identify and quantify low-populated reaction intermediates, thus yielding direct chemical information on reaction mechanisms in real-time assays.

Study of molecular interactions with 13C DNP-NMR

“…Notably, DNP-NMR permits that ligand binding to enzymes can be assessed not only as a molecular interaction, but also via realtime activity assays [24][25][26][27][28] of enzyme in presence and absence of an inhibitor. As an example, the inhibition of jack bean urease by acetohydroxamate was monitored with real-time initial rate assays of urease activity in vitro.…”

Optimizing dissolution dynamic nuclear polarization

“…Although most peer-reviewed papers utilizing d-DNP are concerned with localized spectroscopy or imaging of tumours following the infusion of hyperpolarized 1-13 C pyruvate, the technology of d-DNP can in principle be used in combination with a variety of other NMR or MRI experiments, using an extensive diversity of molecules, and addressing many other nuclear spins. One can cite amongst many other publications in vitro or in-cell studies [13,14], drug screening [15], and monitoring of chemical reactions including the detection of intermediates [16]. Further d-DNP NMR applications can be found in reference [17].…”

Dissolution Dynamic Nuclear Polarization of Non-Self-Glassing Agents: Spectroscopy and Relaxation of Hyperpolarized [1-13C]Acetate

“…2 panel b)) for Na- Table 1) and Tris-[1-13 C]acetate (formulation reported by Jensen et al 17 ). As can be seen, the Tris-acetate formulation has a 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56 57 58 Table 1) and [1- On the contrary, the hyperpolarization process was faster in the case of Na-[1-13 C]acetate (τ = 437 ± 18 s, n = 4) than for [1-13 C]pyruvate (τ = 874 ± 71 s, n = 5).…”