Detection of Listeria species in refrigerated chicken carcasses using Clearview tm and a modified conventional culture method

Abstract

The occurence of Listeria species in refrigerated chicken carcasses was evaluated, comparing the conventional culture methodology of FDA, modified by the introduction of a secondary enrichment step prior plating, and the Clearview TM rapid method (Oxoid, UK Ltd). Forty-eight refrigerated whole chicken carcasses purchased from supermarkets in Florianópolis, SC, Brazil, were analysed. Listeria species occured in 21 (43.7%) samples. Using the Clearview method, 17 (35.4%) samples were positive for Listeria species. Of these isolates, 11 (23%) were L. monocytogenes, 4 (8.3%) L. innocua, 1 (2.1%) L. welshimeri and 1 (2.1%) L. seeligeri. Using the conventional culture methodology of FDA (with modifications), 14 (29.2%) samples were positive for Listeria species. Among these 7 (14.6%) were L. monocytogenes, 6 (12.5%) L. innocua and 1 (2.1%) L. seeligeri. With the Clearview rapid method plus API Listeria for identification, results were confirmed to Listeria species level within 115-139 h. Using the conventional culture method of FDA (with modifications) plus API Listeria, results were confirmed within 120-160 h. However, the Clearview method could indicate the presence of Listeria organisms in only 43 h. Results given by the methods were in moderate concordance and the differences between them were not significant (C.I. = 95%).

Incidence and sources of Listeria monocytogenes in a traditional hot‐smoked rainbow trout processing plant in Turkey†

“…Therefore, the four false-negative samples found by LRT could be ascribed to insufficient antigen extraction below the minimum sensitivity of the test due to the small amount of growth in the LEB enrichment step. Similar situation resulted in falsenegative results by LRT due to the insufficient growth was reported by Pelisser et al (2001) for the isolation of Listeria spp. from chicken samples.…”

Crescimento microbiano em produtos à base de peito de frango durante simulação da cadeia de abastecimento

“…Pode crescer numa temperatura mínima média de 1 °C, significando que pode desenvolver-se em carne refrigerada (MURIANA, 1996;PELISSER, 2001). As amostras de filé de peito cozido de frango (PC) analisadas apresentaram ausência de Listeria monocytogenes durante os 21 dias mantidos a 4 °C, atestando segurança do produto quanto à listeriose.…”

Main Groups of Microorganisms of Relevance for Food Safety and Stability

“…The main suggested niches for Listeria species are soil and water although plants, animals (particularly farm animals), and man are also considered as reservoirs of Listeria spp. (Linke et al, 2014;Pelisser, Mendes, Sutherland, & Batista, 2001).…”