Detection of Listeria monocytogenes in Salmon Using the Probelia Polymerase Chain Reaction System

Wan, Jason; King, Kerryn; Forsyth, Santina; Coventry, M.J.

doi:10.4315/0362-028x-66.3.436

Cited by 17 publications

(4 citation statements)

References 11 publications

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“…The BAX® Listeria monocytogenes Detection System is adopted by the USDA ( 2012 ) as a screening test for L. monocytogenes . Besides, Wan et al ( 2003 ) conducted a study to compare the Probelia® Listeria monocytogenes PCR system and the International Organization for Standardization (ISO) method 11290–1 for the detection of L. monocytogenes in salmon samples. The results indicated that Probelia® Listeria monocytogenes PCR system is as good as the ISO method and the detection limit was approximately 20 CFU/mL broth culture for pure culture of L. monocytogenes.…”

Section: Nucleic Acid-based Methodsmentioning

confidence: 99%

Rapid methods for the detection of foodborne bacterial pathogens: principles, applications, advantages and limitations

et al. 2015

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The incidence of foodborne diseases has increased over the years and resulted in major public health problem globally. Foodborne pathogens can be found in various foods and it is important to detect foodborne pathogens to provide safe food supply and to prevent foodborne diseases. The conventional methods used to detect foodborne pathogen are time consuming and laborious. Hence, a variety of methods have been developed for rapid detection of foodborne pathogens as it is required in many food analyses. Rapid detection methods can be categorized into nucleic acid-based, biosensor-based and immunological-based methods. This review emphasizes on the principles and application of recent rapid methods for the detection of foodborne bacterial pathogens. Detection methods included are simple polymerase chain reaction (PCR), multiplex PCR, real-time PCR, nucleic acid sequence-based amplification (NASBA), loop-mediated isothermal amplification (LAMP) and oligonucleotide DNA microarray which classified as nucleic acid-based methods; optical, electrochemical and mass-based biosensors which classified as biosensor-based methods; enzyme-linked immunosorbent assay (ELISA) and lateral flow immunoassay which classified as immunological-based methods. In general, rapid detection methods are generally time-efficient, sensitive, specific and labor-saving. The developments of rapid detection methods are vital in prevention and treatment of foodborne diseases.

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Section: Nucleic Acid-based Methodsmentioning

confidence: 99%

Rapid methods for the detection of foodborne bacterial pathogens: principles, applications, advantages and limitations

et al. 2015

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“…Few studies that employed the immunological methods were reported. According to previous studies, the great challenge of immunological assays is to eliminate antibody cross-reactivity that can hamper the analysis results [25] . Over recent years, other alternative methods with diverse principles such as chromatography and spectroscopy were developed, aiming to produce more robust results in less analysis time [26,27,28] .…”

Section: Discussionmentioning

confidence: 99%

“…Poucos estudos empregaram os métodos imunológicos. De acordo com a literatura, o grande desafio dos ensaios imunológicos é eliminar a formação de ligações cruzadas que podem dificultar a interpretação do resultado das análises [25] . Nos últimos anos, desenvolveram-se outros métodos alternativos com diversos princípios, como cromatografia e espectroscopia, com o objetivo de produzir resultados mais robustos em menos tempo de análise [26,27,28] .…”

Section: Conflict Of Interestsunclassified

Comparison between Listeria monocytogenes single and multipathogen detection methods

Souza¹,

Ferreira²,

Pasquini-Netto³

et al. 2017

BBR

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143 Biomedical and Biopharmaceutical Research J o r n a l d e I n v e s t i g a ç ã o B i o m é d i c a e B i o f a r m a c ê u t i c a Comparison between Listeria monocytogenes single and multipathogen detection methods Comparação entre os métodos de detecção de Listeria monocytogenes simples e multipatógenos AbstractMultipathogen detection methods have been used to provide a broad range of microorganism detection. We aimed to identify the most searched pathogens in L. monocytogenes detection methods and evaluate if there are sensitivity and specificity differences between single and multipathogen methods. A systematic review was performed by including studies carried out in order to detect L. monocytogenes in a broad range of food sample. A total of 2770 records were retrieved, of which 191 were selected. The majority of the studies (104) presented multipathogen detection, other 87 searched for L. monocytogenes specifically. From the studies for multipathogen detection; other Listeria species (19.4%), Salmonella spp. (21.6%), E. coli O157:H7 (13.2%), Staphylococcus spp. (9.7%), E. coli (5.7%) were more frequently reported. Sensitivity and specificity calculations were derived from only 26 studies, because they compared their data with an official method. Sensitivity and specificity values were close to 100%, showing that others characteristics such as time and cost should be considered to evaluate alternative methods in further research. As a conclusion, evidence generated regarding L. monocytogenes identification methods contribute to method improvements and listeriosis control.Keywords: L. monocytogenes, methods, pathogens, sensitivity, specificity. ResumoOs métodos de detecção para múltiplos patógenos têm sido usados para fornecer uma ampla faixa de detecção de microrganismos. Objetivou-se identificar os patógenos mais buscados nos métodos de detecção para L. monocytogenes e avaliar se existem diferenças nas taxas de sensibilidade e especificidade entre os métodos que buscaram identificar somente L. monocytogenes e os para múltiplos patógenos. Uma revisão sistemática foi realizada incluindo estudos desenvolvidos para detectar L. monocytogenes em amostras alimentares.

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“…It is used to increase sensitivity and specificity and has been used for the identification of L. monocytogenes in clinical samples [57,[107][108][109][110], environmental samples [78], and milk samples [97,111,112]. Commercially available PCR tests are the BAXÒ PCR system (Qualicon, Wilmington, DE, USA) and the ProbeliaÒ assay (Sanofi Diagnostic Pasteur, Marne la Coquette, France), which have been trialed in the field on a variety of different sample types [113][114][115][116][117][118][119][120][121][122].…”

Section: Polymerase Chain Reaction (Pcr)mentioning

confidence: 99%

Methods for the isolation and identification ofListeriaspp. andListeria monocytogenes: a review

Hughes²,

2005

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Listeria monocytogenes is an important food-borne pathogen and is widely tested for in food, environmental and clinical samples. Identification traditionally involved culture methods based on selective enrichment and plating followed by the characterization of Listeria spp. based on colony morphology, sugar fermentation and haemolytic properties. These methods are the gold standard; but they are lengthy and may not be suitable for testing of foods with short shelf lives. As a result more rapid tests were developed based on antibodies (ELISA) or molecular techniques (PCR or DNA hybridization). While these tests possess equal sensitivity, they are rapid and allow testing to be completed within 48 h. More recently, molecular methods were developed that target RNA rather than DNA, such as RT-PCR, real time PCR or nucleic acid based sequence amplification (NASBA). These tests not only provide a measure of cell viability but they can also be used for quantitative analysis. In addition, a variety of tests are available for sub-species characterization, which are particularly useful in epidemiological investigations. Early typing methods differentiated isolates based on phenotypic markers, such as multilocus enzyme electrophoresis, phage typing and serotyping. These phenotypic typing methods are being replaced by molecular tests, which reflect genetic relationships between isolates and are more accurate. These new methods are currently mainly used in research but their considerable potential for routine testing in the future cannot be overlooked.

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Detection of Listeria monocytogenes in Salmon Using the Probelia Polymerase Chain Reaction System

Cited by 17 publications

References 11 publications

Rapid methods for the detection of foodborne bacterial pathogens: principles, applications, advantages and limitations

Rapid methods for the detection of foodborne bacterial pathogens: principles, applications, advantages and limitations

Comparison between Listeria monocytogenes single and multipathogen detection methods

Methods for the isolation and identification ofListeriaspp. andListeria monocytogenes: a review

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