Detection of Listeria monocytogenes from selective enrichment broth using MALDI–TOF Mass Spectrometry

Jadhav, Snehal; Sevior, Danielle; Bhave, Mrinal; Palombo, Enzo A.

doi:10.1016/j.jprot.2013.09.014

Cited by 71 publications

(46 citation statements)

References 26 publications

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“…According to previous studies, the great challenge of immunological assays is to eliminate antibody cross-reactivity that can hamper the analysis results [25] . Over recent years, other alternative methods with diverse principles such as chromatography and spectroscopy were developed, aiming to produce more robust results in less analysis time [26,27,28] . It seems evident that availability of fast and reliable detection methods is critical for microorganism identification, regardless if the method is for single or multiplex detection.…”

Section: Discussionmentioning

confidence: 99%

“…De acordo com a literatura, o grande desafio dos ensaios imunológicos é eliminar a formação de ligações cruzadas que podem dificultar a interpretação do resultado das análises [25] . Nos últimos anos, desenvolveram-se outros métodos alternativos com diversos princípios, como cromatografia e espectroscopia, com o objetivo de produzir resultados mais robustos em menos tempo de análise [26,27,28] . Parece evidente que a disponibilidade de métodos de detecção rápidos e fiáveis é fundamental para a identificação de microrganismos, independentemente se o método é para detecção única ou multiplex.…”

Section: Conflict Of Interestsunclassified

See 1 more Smart Citation

Comparison between Listeria monocytogenes single and multipathogen detection methods

Souza¹,

Ferreira²,

Pasquini-Netto³

et al. 2017

BBR

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143 Biomedical and Biopharmaceutical Research J o r n a l d e I n v e s t i g a ç ã o B i o m é d i c a e B i o f a r m a c ê u t i c a Comparison between Listeria monocytogenes single and multipathogen detection methods Comparação entre os métodos de detecção de Listeria monocytogenes simples e multipatógenos AbstractMultipathogen detection methods have been used to provide a broad range of microorganism detection. We aimed to identify the most searched pathogens in L. monocytogenes detection methods and evaluate if there are sensitivity and specificity differences between single and multipathogen methods. A systematic review was performed by including studies carried out in order to detect L. monocytogenes in a broad range of food sample. A total of 2770 records were retrieved, of which 191 were selected. The majority of the studies (104) presented multipathogen detection, other 87 searched for L. monocytogenes specifically. From the studies for multipathogen detection; other Listeria species (19.4%), Salmonella spp. (21.6%), E. coli O157:H7 (13.2%), Staphylococcus spp. (9.7%), E. coli (5.7%) were more frequently reported. Sensitivity and specificity calculations were derived from only 26 studies, because they compared their data with an official method. Sensitivity and specificity values were close to 100%, showing that others characteristics such as time and cost should be considered to evaluate alternative methods in further research. As a conclusion, evidence generated regarding L. monocytogenes identification methods contribute to method improvements and listeriosis control.Keywords: L. monocytogenes, methods, pathogens, sensitivity, specificity. ResumoOs métodos de detecção para múltiplos patógenos têm sido usados para fornecer uma ampla faixa de detecção de microrganismos. Objetivou-se identificar os patógenos mais buscados nos métodos de detecção para L. monocytogenes e avaliar se existem diferenças nas taxas de sensibilidade e especificidade entre os métodos que buscaram identificar somente L. monocytogenes e os para múltiplos patógenos. Uma revisão sistemática foi realizada incluindo estudos desenvolvidos para detectar L. monocytogenes em amostras alimentares.

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Section: Discussionmentioning

confidence: 99%

Section: Conflict Of Interestsunclassified

Comparison between Listeria monocytogenes single and multipathogen detection methods

Souza¹,

Ferreira²,

Pasquini-Netto³

et al. 2017

BBR

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show abstract

“…MALDI-TOF Detection of Listeria monocytogenes from selective enrichment broth [130] MALDI-TOF Identification and differentiation of foodrelated bacteria [131] MALDI-TOF Identification of lactobacilli isolated [132] MALDI-TOF Detection of enterotoxins produced by pathogenic strains of the Bacillus cereus group [134] UHPLC-QqTOF Analysis on crude and wine-processed Siegesbeckia pubescens [135] MALDI-TOF Optimization of experimental and modeling parameters for the differentiation of beverage spoiling yeasts [136] MALDI-TOF Identification and differentiation of brewery isolates of Pectinatus sp.…”

Section: Maldi-tof-tofmentioning

confidence: 99%

Advanced Mass Spectrometry

Picó

2015

Advanced Mass Spectrometry for Food Safety and Quality

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“…Microbiological analysis is an essential tool for carrying out tests in accordance with the microbiological criteria established for each food type, as well as being essential for evaluating the actions of different management strategies based on the Hazard Analysis and Critical Control Points (HACCP) system (Stannard 1997;Jasson et al 2010). These analysis, mainly based on the morphological, physiological and The MALDI-TOF MS technology has been used extensively in clinical diagnostics, however, its applicability to detection of foodborne pathogenesis comparatively is less explored (Jadhav et al, 2014;Pavlovic et al, 2013). In this context, our study aimed to evaluate the performance of MALD-TOF MS based identification of pathogens in dairy industry.…”

Section: …………………………………………………………………………………………………… Introduction:-mentioning

confidence: 99%

Application of Maldi- Tof MS Technology to the Identification and Characterization of Pathogens in Dairy Industry.

Boultifat¹,

guendouze²,

hamidechi³

2018

IJAR

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Detection of Listeria monocytogenes from selective enrichment broth using MALDI–TOF Mass Spectrometry

Cited by 71 publications

References 26 publications

Comparison between Listeria monocytogenes single and multipathogen detection methods

Comparison between Listeria monocytogenes single and multipathogen detection methods

Advanced Mass Spectrometry

Application of Maldi- Tof MS Technology to the Identification and Characterization of Pathogens in Dairy Industry.

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