Detection of Leishmania (Viannia) DNA in blood from patients with American cutaneous leishmaniasis

Venazzi, Eneide Aparecida Sabaini; Roberto, Andréa Claudia Bekner Silva; Barbosa-Tessmann, Ione Parra; Zanzarini, Paulo Donizete; Lonardoni, Maria Valdrinez Campana; Silveira, Thaís Gomes Verzignassi

doi:10.1016/j.exppara.2006.10.002

Cited by 25 publications

(25 citation statements)

References 17 publications

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“…1 Indeed, several lines of evidence support that lymphatogenous and hematogenous dissemination of the parasite may occur, 1,[6][7][8][9][10] and that nonfocal reservoirs of parasite persistence may actually protect against future reinfection. 9,11,12 Theoretically, then, parasites in both CL and ML would have access to the circulatory compartment of the host, as supported by numerous studies of CL showing Leishmania DNA in the blood, [7][8][9][10] and may therefore be detectable in urine. Cell-free DNA and small DNA fragments of 150-250 bp derived from human circulation can be detected in urine.…”

Section: Introductionmentioning

confidence: 99%

Polymerase Chain Reaction Detection of Leishmania kDNA from the Urine of Peruvian Patients with Cutaneous and Mucocutaneous Leishmaniasis

Veland¹,

Espinosa²,

Valencia³

et al. 2011

The American Society of Tropical Medicine and Hygiene

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Abstract. We hypothesized that Leishmania kDNA may be present in urine of patients with cutaneous leishmaniasis (CL). Urine samples and standard diagnostic specimens were collected from patients with skin lesions. kDNA polymerase chain reaction (PCR) was performed on samples from patients and 10 healthy volunteers from non-endemic areas. Eighty-six of 108 patients were diagnosed with CL and 18 (21%) had detectable Leishmania Viannia kDNA in the urine. Sensitivity and specificity were 20.9% (95% confidence interval [CI] 12.3-29.5%) and 100%. Six of 8 patients with mucocutaneous involvement had detectable kDNA in urine versus 12 of 78 patients with isolated cutaneous diseaseNo healthy volunteer or patient with an alternate diagnosis had detectable kDNA in urine. Sensitivity of urine PCR is sub-optimal for diagnosis. On the basis of these preliminary data in a small number of patients, detectable kDNA in urine may identify less localized forms of infection and inform treatment decisions.

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Section: Introductionmentioning

confidence: 99%

Polymerase Chain Reaction Detection of Leishmania kDNA from the Urine of Peruvian Patients with Cutaneous and Mucocutaneous Leishmaniasis

Veland¹,

Espinosa²,

Valencia³

et al. 2011

The American Society of Tropical Medicine and Hygiene

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“…Several authors have described the detection of Leishmania (Viannia) DNA in peripheral blood as an appropriate tool for the diagnosis of ACL (Ferreira et al, 2006;Venazzi et al, 2007;Martins et al, 2010). However, parasite DNA was not detected in the blood of individuals analyzed in this study.…”

Section: Resultsmentioning

confidence: 99%

“…DNA was extracted by the guanidine-phenol method (Venazzi et al, 2007), resuspended in 50 µL TE buffer (10 mM Tris, 1 mM EDTA; pH 8.0) and stored at 4°C until further use. One positive control (10 4 L. (V.) braziliensis promastigotes in normal human blood) and one negative control (normal human blood) was included for each group of samples extracted.…”

Section: Dna Extractionmentioning

confidence: 99%

Serological and Molecular Investigation of Cutaneous Leishmaniasis in Healthy Individuals from an American Cutaneous Leishmaniasis-Endemic Region

Ribas-Silva

Navasconi

Braga

et al. 2015

American Journal of Infectious Diseases

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American Cutaneous Leishmaniasis (ACL) is a major, clinically relevant zoonosis in Brazil. The disease spectrum includes single, localized, cutaneous ulcers, diffuse cutaneous leishmaniasis and mucosal disease. A subclinical form of the disease has also been described in individuals living in ACL-endemic regions. The goal of this study was to employ immunological and molecular diagnostic methods to evaluate the presence of subclinical ACL in healthy individuals from an ACL-endemic region in northwestern Paraná.

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“…11,12,21 Venazzi and others, 12 studying 68 patients with CL, detected parasite DNA in 3.4% of prepared blood samples. In that study, blood samples were treated with a solution containing 25 mM citric acid, 50 mM sodium citrate, and 81 mM glucose before DNA isolation.…”

mentioning

confidence: 99%

“…9 More sensitive polymerase chain reaction (PCR)-based methods have been developed as an alternative for diagnosis and identification of Leishmania species in clinical samples. 10 PCR-based diagnosis has been used with cutaneous lesions and isolated white blood cells, 11,12 but not with whole blood. Given the limitations of standard methods for the diagnosis and identification of Leishmania species in clinical samples, we compared the diagnostic efficacy of traditional microscopy to PCR-based methods and DNA sequencing using whole blood and skin samples from patients with suspected leishmaniasis.…”

mentioning

confidence: 99%

Diagnostic Efficacy of Molecular Techniques for Detection and Identification of Leishmania Species in Human Whole Blood and Skin Samples from Ecuador

Muñoz

Santander

Rojas-Silva

et al. 2016

The American Society of Tropical Medicine and Hygiene

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Abstract. Microscopic examination is the standard method for diagnosis of cutaneous and mucocutaneous leishmaniasis despite its low sensitivity. This study compared the diagnosis efficacy of microscopic examination versus polymerase chain reaction (PCR)-based methods and DNA sequencing using whole blood and skin lesion samples from patients with suspected leishmaniasis. The presence of Leishmania was determined by microscopy and amplification of 18S ribosomal RNA gene from blood and skin samples of 22 patients. Twenty individuals were positive for leishmaniasis. Microscopic analysis identified 85%, whereas PCR identified 100% of positive cases from skin and 90% from blood. Cytochrome b gene (cyt-b) amplification and sequencing identified Leishmania guyanensis, Leishmania shawi, and Leishmania naiffi from skin and blood samples. This study demonstrated the usefulness of whole blood and molecular techniques for the diagnosis and species identification of leishmaniasis.Leishmaniasis is a vector-borne disease caused by protozoan parasites of the Leishmania genus.

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Detection of Leishmania (Viannia) DNA in blood from patients with American cutaneous leishmaniasis

Cited by 25 publications

References 17 publications

Polymerase Chain Reaction Detection of Leishmania kDNA from the Urine of Peruvian Patients with Cutaneous and Mucocutaneous Leishmaniasis

Polymerase Chain Reaction Detection of Leishmania kDNA from the Urine of Peruvian Patients with Cutaneous and Mucocutaneous Leishmaniasis

Serological and Molecular Investigation of Cutaneous Leishmaniasis in Healthy Individuals from an American Cutaneous Leishmaniasis-Endemic Region

Diagnostic Efficacy of Molecular Techniques for Detection and Identification of Leishmania Species in Human Whole Blood and Skin Samples from Ecuador

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