Detection of lactoferrin in bovine and goat milk by enzyme-linked immunosorbent assay

Chen, P.-W.; Mao, Frank Chiahung

doi:10.38212/2224-6614.2653

Cited by 7 publications

(4 citation statements)

References 29 publications

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“…Cow's milk is the most readily available source of lactoferrin, with an average concentration of 0.174 g/l in low heat pasteurized cow's milk (and 1.2 g/kg in semihard cheese produced from that milk) [129], which is in good agreement with other studies [130][131][132], though the range experienced (0.03-0.486 g/l) is dependent on several factors [133]. The concentration in colostrum is higher, but varies greatly between breeds and may be anything between 0.3 and 5 g/l, and is typically at the lower end of the range [134][135][136].…”

Section: Sources Forms and Practical Use Of Lactoferrin Ovotransferrin And Lysozymesupporting

confidence: 89%

The Potential of lactoferrin, Ovotransferrin and Lysozyme As Antiviral and immune-modulating Agents in COVID-19

Mann

Ndung’u

2020

Future Virol.

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Coronavirus disease 2019 (COVID-19), caused by SARS coronavirus 2 (SARS-CoV-2), is spreading rapidly with no established effective treatments. While most cases are mild, others experience uncontrolled inflammatory responses with oxidative stress, dysregulation of iron and coagulation as features. Lactoferrin, ovotransferrin and lysozyme are abundant, safe antimicrobials that have wide antiviral as well as immunomodulatory properties. In particular, lactoferrin restores iron homeostasis and inhibits replication of SARS-CoV, which is closely related to SARS-CoV-2. Ovotransferrin has antiviral peptides and activities that are shared with lactoferrin. Both lactoferrin and lysozyme are ‘immune sensing’ as they may stimulate immune responses or resolve inflammation. Mechanisms by which these antimicrobials may treat or prevent COVID-19, as well as sources and forms of these, are reviewed.

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Section: Sources Forms and Practical Use Of Lactoferrin Ovotransferrin And Lysozymesupporting

confidence: 89%

The Potential of lactoferrin, Ovotransferrin and Lysozyme As Antiviral and immune-modulating Agents in COVID-19

Mann

Ndung’u

2020

Future Virol.

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“…The biosensor used by Indyk and Filonzi (2005) had a working range of 0–1000 ng/mL and a method detection limit (MDL) of 19.9 μg/mL in undiluted milk with an analysis time of ~8 min, offering good potential for various dairy applications with further dilution if required. The authors used the biosensor to quantify the Lf content in 10 consumer bovine milk samples and found a range of 150–210 μg/mL, which is within reported levels based on literature values (Chen and Mao 2004; Cheng et al . 2008).…”

Section: Application Of Biosensors For Analysis Of Milk Proteinssupporting

confidence: 75%

Use of biosensor technology in analysing milk and dairy components: A review

Oh¹,

Eathorne²,

Jones³

2022

Int J of Dairy Tech

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Biosensor technology is relatively new to the dairy industry although it has been used successfully elsewhere including medical devices. This review summarises biosensors with different combinations of biological receptors and transducers which were used to analyse components in milk. Quantification of individual milk proteins and specific detection of lactose were clearly demonstrated. Strain‐specific enumeration of pathogens were also reported along with analyses of raw milk quality parameters relevant for processing. The advantages of using biosensors were consistently shown across numerous studies. Biosensors provide rapid, simple and analyte‐specific techniques, offering unique solutions to support continued innovation in dairy products and processes.

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“…Even though LF is not an APP, it increases remarkably during the inflammatory response due to its production by mammary epithelial cells (Galfi et al 2016a ). Concerning test characteristics for goats and cows, two studies carried out a competitive ELISA by using a lactoferrin antiserum from rabbit, and goat lactoferrin was isolated and purified (Chen and Mao 2004 ; Chen et al 2004 ). In other studies, cow LF was quantified by a commercial sandwich LF ELISA kit (Bethyl Laboratories, Montgomery, TX) (Cheng et al 2008 ; Sobczuk-Szul et al 2014 ; Galfi et al 2016a , 2016b ).…”

Section: Resultsmentioning

confidence: 99%

Milk proteins as mastitis markers in dairy ruminants - a systematic review

et al. 2022

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Mastitis is one of the most impacting diseases in dairy farming, and its sensitive and specific detection is therefore of the greatest importance. The clinical evaluation of udder and mammary secretions is typically combined with the milk Somatic Cell Count (SCC) and often accompanied by its bacteriological culture to identify the causative microorganism. In a constant search for improvement, several non-enzymatic milk proteins, including milk amyloid A (M-SAA), haptoglobin (HP), cathelicidin (CATH), and lactoferrin (LF), have been investigated as alternative biomarkers of mastitis for their relationship with mammary gland inflammation, and immunoassay techniques have been developed for detection with varying degrees of success. To provide a general overview of their implementation in the different dairy species, we carried out a systematic review of the scientific literature using the PRISMA (Preferred Reporting Items for Systematic Reviews and Meta-Analysis) guidelines. Our review question falls within the type “Diagnostic test accuracy questions” and aims at answering the diagnostic question: “Which are the diagnostic performances of mastitis protein biomarkers investigated by immunoassays in ruminant milk?”. Based on 13 keywords combined into 42 searches, 523 manuscripts were extracted from three scientific databases. Of these, 33 passed the duplicate removal, title, abstract, and full-text screening for conformity to the review question and document type: 78.8% investigated cows, 12.1% sheep, 9.1% goats, and 6.1% buffaloes (some included more than one dairy species). The most frequently mentioned protein was M-SAA (48.5%), followed by HP (27.3%), CATH (24.2%) and LF (21.2%). However, the large amount of heterogeneity among studies in terms of animal selection criteria (45.5%), index test (87.9%), and standard reference test (27.3%) resulted in a collection of data not amenable to meta-analysis, a common finding illustrating how important it is for case definitions and other criteria to be standardized between studies. Therefore, results are presented according to the SWiM (Synthesis Without Meta-analysis) guidelines. We summarize the main findings reported in the 33 selected articles for the different markers and report their results in form of comparative tables including sample selection criteria, marker values, and diagnostic performances, where available. Finally, we report the study limitations and bias assessment findings.

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Detection of lactoferrin in bovine and goat milk by enzyme-linked immunosorbent assay

Cited by 7 publications

References 29 publications

The Potential of lactoferrin, Ovotransferrin and Lysozyme As Antiviral and immune-modulating Agents in COVID-19

The Potential of lactoferrin, Ovotransferrin and Lysozyme As Antiviral and immune-modulating Agents in COVID-19

Use of biosensor technology in analysing milk and dairy components: A review

Milk proteins as mastitis markers in dairy ruminants - a systematic review

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