Detection of KPC in
            <i>Acinetobacter</i>
            spp. in Puerto Rico

Robledo, Iraida E.; Aquino, Edna E.; Santé, María I.; Santana, Jorge; Otero, Diana M.; León, Carlos F.; Vázquez, Guillermo J.

doi:10.1128/aac.00899-09

Cited by 174 publications

(105 citation statements)

References 29 publications

(35 reference statements)

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“…In contrast, our results were different from those found for carbapenem resistance in isolates in Chile (6.5 %), Mexico (1.1 %) or Guatemala, Honduras, Panama, Puerto Rico and Jamaica where no carbapenem-resistant isolates were found (Tognim et al, 2004). These percentages may have increased more recently as reported from hospitals in Puerto Rico, where imipenem-resistant isolates have emerged recently (Robledo et al, 2010). Reports from Asia-Pacific nations and Europe have revealed on average a lower percentage of carbapenem-resistant isolates (Mendes et al, 2009;Souli et al, 2008).…”

Section: Resultsmentioning

confidence: 88%

Emergence and clonal dissemination of carbapenem-hydrolysing OXA-58-producing Acinetobacter baumannii isolates in Bolivia

Sevillano

Fernández

Bustamante

et al. 2012

Journal of Medical Microbiology

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and the presence of carbapenemases as a mechanism of resistance to imipenem. Isolates were obtained from 46 patients (one isolate per patient; 30 males,16 females) with an age range of 1 day to 84 years, and were collected from different sample types, the majority from respiratory tract infections (17) and wounds (13). Resistance to imipenem was detected in 15 isolates collected from different hospitals of the city. These isolates grouped into the same genotype, named A, and were resistant to all antibiotics tested including imipenem, with susceptibility only to colistin. Experiments to detect carbapenemases revealed the presence of the OXA-58 carbapenemase. Further analysis revealed the location of the bla OXA-58 gene on a 40 kb plasmid. To our knowledge, this is the first report of carbapenem resistance in A. baumannii isolates from Bolivia that is conferred by the OXA-58 carbapenemase. The presence of this gene in a multidrug-resistant clone and its location within a plasmid is of great concern with regard to the spread of carbapenem-resistant A. baumannii in the hospital environment in Bolivia. INTRODUCTIONAcinetobacter baumannii is a nosocomial pathogen responsible for severe infections often diagnosed in patients attending hospitals worldwide. Carbapemens are considered the drugs of choice for treating these infections, but they are not always efficient due to the occurrence of multidrug-resistant strains of this bacterium (Canduela et al., 2006;Coelho et al., 2006;Higgins et al., 2010a;Mendes et al., 2009;Poirel et al., 2010). Mechanisms enabling the development of carbapenem resistance include an induced decrease in membrane permeability, efflux pump overexpression and the production of carbapenemases. Among these mechanisms, the production of carbapenemases plays a major role in carbapenem resistance in most Gram-negative bacilli, including A. baumannii clinical isolates (Canduela et al., 2006;Coelho et al., 2006;Poirel et al., 2010;Queenan & Bush, 2007;Walsh, 2010). The most common type of enzyme occurring in A. baumannii is represented by the carbapenem-hydrolysing class D b-lactamases, which are divided into five phylogenetic groups: OXA-23-like, OXA-24-like, OXA-58-like, OXA-51-like, which is intrinsic to A. baumannii, and the recently described OXA-143-like enzymes (Higgins et al., 2009;Queenan & Bush, 2007).Although A. baumannii is considered an emerging pathogen and much research has been carried out on it, there have been only a few studies concerning the mechanisms conferring carbapenem resistance of this bacterium in Latin American countries (Celenza et al., 2006;Coelho et al., 2006;Sader et al., 2004;Sgambatti et al., 2010). In addition, there are no previous publications regarding the resistance of A. baumannii to imipenem or the occurrence of carbapenemases in A. baumannii isolates in Bolivia.In this study, we analysed the antibiotic resistance, in particular to imipenem, of clinical isolates of A. baumannii obtained from several hospitals in Cochabamba, Bolivia, and the presence of carbape...

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Section: Resultsmentioning

confidence: 88%

Emergence and clonal dissemination of carbapenem-hydrolysing OXA-58-producing Acinetobacter baumannii isolates in Bolivia

Sevillano

Fernández

Bustamante

et al. 2012

Journal of Medical Microbiology

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“…The presence of the genes encoding KPC, MBLs of IMP, VIM, SIM and NDM class [4,[5][6][7][8] and OXA β-lactamases (bla OXA-51-like , bla OXA-23-like , bla OXA-40-like , bla OXA-58-like and bla OXA-143-5 like ) genes [18] was determined by PCR as previously described. The amplicons of the selected representative isolates were column-purified (QIAquick PCR purification kit, Inel Medicinska tehnika, Zagreb) and subjected to sequencing by the Macrogen sequencing service (South Korea) with the same primers used for PCR in order to determine the identity of the enzyme.…”

Section: 3molecular Characterization Of β-Lactamasesmentioning

confidence: 99%

“…Rarely, resistance can occurr because of porin loss (CarO) or overexpression of efflux pumps [3]. Acquired carbapenem resistance in A. baumannii can also be mediated by acquired carbapenemases of class A (KPC) [4], metallo-β-lactamases (MBLs) of IMP, VIM, SIM and NDM family [5][6][7][8] and carbapenem-hydrolyzing oxacillinases (CHDL) (OXA-23-like, OXA-40-like, OXA-58-like, OXA-143-like and OXA-235-like [9][10][11][12]. From 2008 carbapenem-resistant A. baumannii emerged with increasing frequency in most hospital centers in Croatia and became an important public health problem as shown by the multicenter study conducted in Northern Croatia and Istria demonstrating that OXA-40-like and OXA-58-like were the dominant types of acquired CHDL in Croatia.…”

Section: Introductionmentioning

confidence: 99%

Emergence of different Acinetobacter baumannii clones in a Croatian hospital and correlation with antibiotic susceptibility

Ladavac

Bedenić

Vranić-Ladavac³

et al. 2017

Journal of Global Antimicrobial Resistance

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“…Carbapenemases in Acinetobacter spp. belong to Ambler class A (KPC and some GES variants) (Bonnin et al, 2013b;Robledo et al, 2010), class B (VIM, IMP, SIM, NDM) (Bonnin et al, 2012;Poirel and Nordmann, 2006), and mostly class D (oxacillinases) (Bonnin et al, 2013a). Five main groups of oxacillinases with carbapenemase activity have been identified in A. baumannii, i.e., the intrinsic chromosomal OXA-51-like and the acquired chromosomally and plasmid-encoded OXA-23-like, OXA-40-like, OXA-58-like, and OXA-143-like enzymes (Bonnin et al, 2013a;Higgins et al, 2009;Poirel and Nordmann, 2006).…”

mentioning

confidence: 99%

CHROMagar Acinetobacter medium for detection of carbapenemase-producing Acinetobacter spp. strains from spiked stools

Girlich

Nordmann

2015

Diagnostic Microbiology and Infectious Disease

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Detection of KPC in Acinetobacter spp. in Puerto Rico

Cited by 174 publications

References 29 publications

Emergence and clonal dissemination of carbapenem-hydrolysing OXA-58-producing Acinetobacter baumannii isolates in Bolivia

Emergence and clonal dissemination of carbapenem-hydrolysing OXA-58-producing Acinetobacter baumannii isolates in Bolivia

Emergence of different Acinetobacter baumannii clones in a Croatian hospital and correlation with antibiotic susceptibility

CHROMagar Acinetobacter medium for detection of carbapenemase-producing Acinetobacter spp. strains from spiked stools

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