Detection of koi herpesvirus in common carp, <i>Cyprinus carpio</i> L., by loop‐mediated isothermal amplification

Gunimaladevi, I.; Kono, Tomoya; Venugopal, M. N.; Sakai, Masahiro

doi:10.1111/j.1365-2761.2004.00578.x

Cited by 80 publications

(60 citation statements)

References 13 publications

(23 reference statements)

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“…The figures presented in this study suggest that the LAMP assay is 1,000 times more sensitive than PCR for detection of ETEC from raw milk. Most studies using the LAMP reaction for detection have found sensitivity to be equal to or 10 times more than conventional PCR/ RT-PCR (17)(18)(19)(20).…”

Section: Discussionmentioning

confidence: 99%

Detection methods for milk pathogenic bacteria by loop-mediated isothermal amplification

Yang

Song

Wang

et al. 2014

BST

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Section: Discussionmentioning

confidence: 99%

Detection methods for milk pathogenic bacteria by loop-mediated isothermal amplification

Yang

Song

Wang

et al. 2014

BST

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“…A set of four primers, two inner and two outer, were designed based on the sequence of the thymidine kinase (tk) gene of KHV. This diagnostic method could be very useful in routine diagnostics and in surveillance/quarantine procedures (Gunimaladevi et al, 2004).…”

Section: Diagnostic Methodsmentioning

confidence: 99%

Current knowledge on koi herpesvirus (KHV) - a review

Pokorová¹,

Veselý²,

Piačková³

et al. 2005

Vet. Med.

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ABSTRACT:The first outbreaks of a disease connected with high mortality of common carp and koi carp caused by koi herpesvirus (KHV) were reported in 1998 in Israel and in the United States. Since then, several cases have been confirmed all over the world. At present, this viral disease is considered to be one of the most risky factors affecting populations of common carp and koi carp. Affected fish become disoriented and swim erratically with high breathing frequency, swollen gills and partially local skin lesions. The virus was isolated from the tissues of fish showing signs of the disease and subsequently cultured on koi fin (KF-1) cells. Electron microscopic examinations revealed morphological signs identical with viruses of the family Herpesviridae. Analysis of virion polypeptides and gene DNA showed the differences between KHV and the well-known herpesvirus of cyprinids, Herpesvirus cyprini (CHV), and Channel catfish virus (CCV). Water temperature is a factor influencing the onset and severity of disease. Fish seem most susceptible at water temperatures of 18-28°C, no morbidities occur at 13°C and 30°C. At present, diagnosis of KHV is mainly based on detection of viral DNA by PCR method.

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“…LAMP assays have been used previously for rapid diagnosis of the causative agents of different diseases which affect aquaculture (Caipang et al 2004, Gunimaladevi et al 2004, El-Matbouli & Soliman 2005a,b, Soliman & El-Matbouli 2005. Our LAMP assay was optimised to amplify T. contejeani DNA using a set of 6 primers incubated at 65°C for 60 min in the presence of a Bst DNA polymerase enzyme.…”

Section: Discussionmentioning

confidence: 99%

Molecular diagnostic methods for detection of Thelohania contejeani (Microsporidia), the causative agent of porcelain disease in crayfish

El-Matbouli¹,

Soliman²

2006

Dis. Aquat. Org.

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Diagnosis of Thelohania contejeani in the crayfish Astacus astacus is currently based on observation of gross clinical signs -opaque appearance of the abdomen and whitish colouration of the musculature -and confirmed by microscopic examination of histological sections of muscle. We have developed 2 molecular diagnostic methods for sensitive and rapid detection of porcelain disease in its early stages: PCR and loop-mediated isothermal amplification (LAMP). The PCR test utilises a primer based on the T. contejeani small subunit ssu ribosomal RNA (ssu rRNA) gene and amplified parasite DNA with high specificity and a detection limit of 10 -5 dilution. The LAMP assay involves incubation of the target DNA with a set of 6 primers and Bst DNA polymerase for 60 min at 65°C in a water bath or heating block, followed by visualisation of the reaction products with the SYBR Green I stain; sensitivity of visual detection with SYBR Green I is equivalent to that with agarose gel electrophoresis. The LAMP assay can detect T. contejeani DNA to a dilution of 10 -7 . The LAMP assay is 100 times more sensitive than the PCR test and is the method we recommend as an alternative to traditional means of diagnosing T. contejeani. KEY WORDS: Thelohania contejeani · PCR · Loop-mediated isothermal amplification · Diagnosis · CrayfishResale or republication not permitted without written consent of the publisher

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Detection of koi herpesvirus in common carp, Cyprinus carpio L., by loop‐mediated isothermal amplification

Cited by 80 publications

References 13 publications

Detection methods for milk pathogenic bacteria by loop-mediated isothermal amplification

Detection methods for milk pathogenic bacteria by loop-mediated isothermal amplification

Current knowledge on koi herpesvirus (KHV) - a review

Molecular diagnostic methods for detection of Thelohania contejeani (Microsporidia), the causative agent of porcelain disease in crayfish

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