Detection of Kobe-type<i>Babesia microti</i>associated with Japanese human babesiosis in field rodents in central Taiwan and southeastern mainland China

Saito-Ito, Atsuko; Takada, Nobuhiro; Ishiguro, F.; Fujita, Hiromi; Yano, Yasuhiro; Ma, Xin; Chen, E.-R.

doi:10.1017/s0031182008004356

Cited by 27 publications

(34 citation statements)

References 34 publications

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“…B. microti was detected in I. persulcatus , H. longicornis , and H. concinna in Fujian, Zhejiang, Henan, and Heilongjiang provinces (Saito-Ito et al, 2008; Sun et al, 2008; Zhou et al, 2014). B. venatorum has been reported in I. persulcatus ticks collected from forested areas of northeastern China (Jiang et al, 2015).…”

Section: Discussionmentioning

confidence: 99%

Molecular Detection and Characterization of Zoonotic and Veterinary Pathogens in Ticks from Northeastern China

et al. 2016

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Tick-borne diseases are considered as emerging infectious diseases in humans and animals in China. In this study, Ixodes persulcatus (n = 1699), Haemaphysalis concinna (n = 412), Haemaphysalis longicornis (n = 390), Dermacentor nuttalli (n = 253), and Dermacentor silvarum (n = 204) ticks were collected by flagging from northeastern China, and detected for infection with Anaplasma, Ehrlichia, Babesia, and Hepatozoon spp. by using nested polymerase chain reaction assays and sequencing analysis. Anaplasma phagocytophilum was detected in all tick species, i.e., I. persulcatus (9.4%), H. longicornis (1.9%), H. concinna (6.5%), D. nuttalli (1.7%), and D. silvarum (2.3%); Anaplasma bovis was detected in H. longicornis (0.3%) and H. concinna (0.2%); Ehrlichia muris was detected in I. persulcatus (2.5%) and H. concinna (0.2%); Candidatus Neoehrlichia mikurensis was only detected in I. persulcatus (0.4%). The Ehrlichia variant (GenBank access number KU921424), closely related to Ehrlichia ewingii, was found in H. longicornis (0.8%) and H. concinna (0.2%). I. persulcatus was infected with Babesia venatorum (1.2%), Babesia microti (0.6%), and Babesia divergens (0.6%). Additionally, four Babesia sequence variants (GenBank access numbers 862303–862306) were detected in I. persulcatus, H. longicornis, and H. concinna, which belonged to the clusters formed by the parasites of dogs, sheep, and cattle (B. gibsoni, B. motasi, and B. crassa). Two Hepatozoon spp. (GenBank access numbers KX016028 and KX016029) associated with hepatozoonosis in Japanese martens were found in the collected ticks (0.1–3.1%). These findings showed the genetic variability of Anaplasma, Ehrlichia, Babesia, and Hepatozoon spp. circulating in ticks in northeastern China, highlighting the necessity for further research of these tick-associated pathogens and their role in human and animal diseases.

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Section: Discussionmentioning

confidence: 99%

Molecular Detection and Characterization of Zoonotic and Veterinary Pathogens in Ticks from Northeastern China

et al. 2016

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“…While several species of Babesia can infect humans, Babesia microti and Babesia divergens are the species most often associated with human infection (187,229,281). Small mammals (235,307,492,539,574) and ruminants (275,354,501,542,574) serve as reservoirs for Babesia spp., and humans usually become infected after an infected Ixodes tick takes a blood meal. However, transmission via blood transfusion has also been widely reported (50,86,246,272,366,379).…”

Section: Babesia Sppmentioning

confidence: 99%

“…Interestingly, a mouse trapped near the residence of the blood donor was infected with a Babesia isolate with an rDNA sequence identical to that of the Kobe strain (596). Kobe strain-like Babesia isolates have also been detected in field rodents in central Taiwan and Southeastern Mainland China (492).…”

Section: Babesia Sppmentioning

confidence: 99%

Importance of Nonenteric Protozoan Infections in Immunocompromised People

et al. 2010

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SUMMARY There are many neglected nonenteric protozoa able to cause serious morbidity and mortality in humans, particularly in the developing world. Diseases caused by certain protozoa are often more severe in the presence of HIV. While information regarding neglected tropical diseases caused by trypanosomatids and Plasmodium is abundant, these protozoa are often not a first consideration in Western countries where they are not endemic. As such, diagnostics may not be available in these regions. Due to global travel and immigration, this has become an increasing problem. Inversely, in certain parts of the world (particularly sub-Saharan Africa), the HIV problem is so severe that diseases like microsporidiosis and toxoplasmosis are common. In Western countries, due to the availability of highly active antiretroviral therapy (HAART), these diseases are infrequently encountered. While free-living amoebae are rarely encountered in a clinical setting, when infections do occur, they are often fatal. Rapid diagnosis and treatment are essential to the survival of patients infected with these organisms. This paper reviews information on the diagnosis and treatment of nonenteric protozoal diseases in immunocompromised people, with a focus on patients infected with HIV. The nonenteric microsporidia, some trypanosomatids, Toxoplasma spp., Neospora spp., some free-living amoebae, Plasmodium spp., and Babesia spp. are discussed.

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“…Unlike the rRNA genes which are highly conserved between closely related species, the spacer regions (ITS1 and ITS2) are subject to higher evolutionary rates and are therefore more variable in their lengths and nucleotide composition (Hillis and Dixon, 1991). These regions have therefore been used for the discrimination of closely related species and subspecies, and in the description of new species (Zahler et al, 1998;Holman et al, 2003;Lew et al, 2003;Aktas et al, 2007;de Rojas et al, 2007;Hilpertshauser et al, 2007;Saito-Ito et al, 2008;Niu et al, 2009;Bosman et al, 2010).…”

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confidence: 99%

Phylogeny of Theileria buffeli genotypes identified in the South African buffalo (Syncerus caffer) population

Chaisi

Collins

Oosthuizen

2014

Veterinary Parasitology

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Theileria buffeli/T. sergenti/T. orientalis is a group of benign and mildly pathogenic species of cattle and buffalo. In a previous study, we identified T .buffeli in blood samples originating from the African buffalo (Syncerus caffer) in the Hluhluwe-iMfolozi Game Park (HIP) and the Addo Elephant Game Park (AEGP) in South Africa. The aim of this study was to characterize the 18S rRNA gene and complete internal transcribed spacer (ITS1-5.8S-ITS2) region of T. buffeli samples, and to establish the phylogenetic position of this species based on these loci. The 18S rRNA gene and the complete ITS region were amplified from DNA extracted from blood samples originating from buffalo in these localities. The PCR products were cloned and the resulting recombinants sequenced. We identified novel T. buffeli 18S rRNA gene and ITS genotypes from buffalo in the AEGP, and novel T. sinensis 18S rRNA genotypes from buffalo in the HIP. Phylogenetic analyses indicated that the T. buffeli-like sequences were similar to T. buffeli sequences from cattle and buffalo in China and India, and the T. sinensis-like sequences were similar to T. sinensis 18S rRNA sequences of cattle and yak in China. There was extensive sequence variation between the novel T.1 buffeli genotypes of the African buffalo and previously described T. buffeli and T. sinensis genotypes. The presence of organisms with T. buffeli-like and T. sinensis-like genotypes in the African buffalo could be of significant importance, particularly to the cattle industry in South Africa as these animals might act as sources of infections to naïve cattle. This is the first report on the characterization of the full-length 18S rRNA gene and ITS region of T. buffeli and T. sinensis genotypes in South Africa. Our study provides invaluable information towards the classification of this complex group of benign and mildly pathogenic species.

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Detection of Kobe-typeBabesia microtiassociated with Japanese human babesiosis in field rodents in central Taiwan and southeastern mainland China

Cited by 27 publications

References 34 publications

Molecular Detection and Characterization of Zoonotic and Veterinary Pathogens in Ticks from Northeastern China

Molecular Detection and Characterization of Zoonotic and Veterinary Pathogens in Ticks from Northeastern China

Importance of Nonenteric Protozoan Infections in Immunocompromised People

Phylogeny of Theileria buffeli genotypes identified in the South African buffalo (Syncerus caffer) population

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