Detection of Jingmenviruses in Japan with Evidence of Vertical Transmission in Ticks

Kobayashi, Daisuke; Kuwata, Ryusei; Kimura, Toshiya; Shimoda, Hiroshi; Fujita, Ryosuke; Faizah, Astri Nur; Kai, Izumi; Matsumura, Ryo; Kuroda, Yudai; Watanabe, Shumpei; Kuniyoshi, Sawako; Yamauchi, Takeo; Watanabe, Mamoru; Higa, Yukiko; Hayashi, Toshihiko; Shinomiya, Hiroto; Maeda, Ken; Kasai, Shinji; Sawabe, Kyoko; Isawa, Haruhiko

doi:10.3390/v13122547

Cited by 27 publications

(40 citation statements)

References 47 publications

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“…JMTV isolation attempts suggested only replication for two cell culture passages in Vero E6 and C6/36 cells. While the detection of JMTV RNA in cell culture supernatant may be an indication of virus replication in these cells, it is also possible that the JMTV RNA detections in cell culture supernatants were carryover from tick homogenates as speculated by Kobayashi et al [ 51 ]. Earlier virus isolation attempts have shown mixed outcomes with inconsistent replication in C6/36 as well as in DH82 (canine macrophage) cells, and persistent growth in BME/CTVM23 ( Rh.…”

Section: Discussionmentioning

confidence: 99%

“…Earlier virus isolation attempts have shown mixed outcomes with inconsistent replication in C6/36 as well as in DH82 (canine macrophage) cells, and persistent growth in BME/CTVM23 ( Rh. microplus embryo-derived cell line) [ 1 , 2 , 51 ]. Other jingmenviruses such as ALSV has been shown to replicate in Vero and IRE/CTVM19 ( Ixodes ricinus) cell lines [ 52 , 53 ].…”

Section: Discussionmentioning

confidence: 99%

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Jingmen Tick Virus in Ticks from Kenya

Ogola

Kopp

Bastos

et al. 2022

Viruses

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Jingmen tick virus (JMTV) is an arbovirus with a multisegmented genome related to those of unsegmented flaviviruses. The virus first described in Rhipicephalus microplus ticks collected in Jingmen city (Hubei Province, China) in 2010 is associated with febrile illness in humans. Since then, the geographic range has expanded to include Trinidad and Tobago, Brazil, and Uganda. However, the ecology of JMTV remains poorly described in Africa. We screened adult ticks (n = 4550, 718 pools) for JMTV infection by reverse transcription polymerase chain reaction (RT-PCR). Ticks were collected from cattle (n = 859, 18.88%), goats (n = 2070, 45.49%), sheep (n = 1574, 34.59%), and free-ranging tortoises (Leopard tortoise, Stigmochelys pardalis) (n = 47, 1.03%) in two Kenyan pastoralist-dominated areas (Baringo and Kajiado counties) with a history of undiagnosed febrile human illness. Surprisingly, ticks collected from goats (0.3%, 95% confidence interval (CI) 0.1–0.5), sheep (1.8%, 95% CI 1.2–2.5), and tortoise (74.5%, 95% CI 60.9–85.4, were found infected with JMTV, but ticks collected from cattle were all negative. JMTV ribonucleic acid (RNA) was also detected in blood from tortoises (66.7%, 95% CI 16.1–97.7). Intragenetic distance of JMTV sequences originating from tortoise-associated ticks was greater than that of sheep-associated ticks. Phylogenetic analyses of seven complete-coding genome sequences generated from tortoise-associated ticks formed a monophyletic clade within JMTV strains from other countries. In summary, our findings confirm the circulation of JMTV in ticks in Kenya. Further epidemiological surveys are needed to assess the potential public health impact of JMTV in Kenya.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Jingmen Tick Virus in Ticks from Kenya

Ogola

Kopp

Bastos

et al. 2022

Viruses

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“…Segments 2 and 4 code for two structural proteins each (VP4 and VP1; VP2 and VP3, respectively) and are more genetically distant from flaviviruses than the non-structural proteins. Since this discovery, several other virus species associated with ticks or human infections (i.e., Alongshan virus, Yanggou tick virus, Xinjiang tick virus 1, and Takashi virus), other vertebrates (i.e., bats and rodents), insects, or plants [6][7][8][9][10][11] have been discovered. To date, jingmenviruses have been detected in a wide range of hosts and in geographical locations on four continents (specifically Asia, America, Africa and Europe).…”

Section: Introductionmentioning

confidence: 99%

Discovery of a Novel Jingmenvirus in Australian Sugarcane Soldier Fly (Inopus flavus) Larvae

Colmant

Furlong

Etebari

2022

Viruses

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In Australia, soldier flies are major pests of sugarcane, and they can cause significant yield losses in some areas, possibly due to the virus’ transmission to the plants. We sequenced fly larvae salivary glands and identified a novel jingmenvirus, putatively named Inopus flavus jingmenvirus 1 (IFJV1). Phylogenetic trees confirmed that IFJV1 groups with insect-associated jingmenviruses, newly identified flavivirus-like viruses with a segmented genome. After the design and the validation of molecular detection systems for IFJV1, larval homogenates were passaged on insect and vertebrate cells, but IFJV1 could only be detected in the first two passages in insect cells and not at all in vertebrate cells. Despite this lack of consistent replication in laboratory models, this virus does replicate in its host Inopus flavus, as sequenced, small RNA from the larvae matched the IFJV1 sequences. Moreover, they were found to be predominantly 21 nucleotides long and map to the whole sequences on both strands, which is typical of an actively replicating virus. This discovery confirms the worldwide presence of jingmenviruses which, until now, had only been detected on four continents. However, the study of IFJV1 tropism and the possible pathogenicity to its host or the sugarcane it parasitizes requires the development of a stable replication model.

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“…In China, tick-borne encephalitis virus (TBEV) in the family Flaviviridae and Crimean-Congo hemorrhagic fever virus (CCHFV) in the family Nairoviridae are the two causative agents of viral encephalitis and hemorrhagic fever in northeastern and northwestern regions, respectively, that are transmitted by different tick species (9)(10)(11). Emerging TBVs, such as severe fever with thrombocytopenia virus (SFTSV), Jingmen tick virus (JMTV), Alongshan virus (ALSV), Songling virus (SGLV), Beiji nairovirus (BJNV), Tacheng tick virus 1 and 2, have been reported to be associated with human diseases (12)(13)(14)(15)(16)(17)(18). Nairobi sheep disease virus (NSDV), another member in the family Nairoviridae that can cause an acute hemorrhagic gastroenteritis in sheep and goats, has also been found in ticks in China (19,20).…”

Section: Introductionmentioning

confidence: 99%

Extensive diversity of RNA viruses in ticks revealed by metagenomics in northeastern China

Liu

Li²,

et al. 2022

Preprint

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Recently, several emerging tick-borne viruses have been identified to be associated with human diseases in northeastern China. Here, we used metagenomics to investigate the virome diversity in Haemaphysalis japonica, H. conicinna, Dermacentor silvarum, and Ixodes persulcatus ticks in northeastern China. A total of 22 RNA viruses were identified and belonged to more eight viral families, including four each in Nairoviridae and Phenuiviridae, three each in Flaviviridae, Rhabdoviridae, and Solemoviridae, two in Chuviridae, and one each in Partitiviridae, Tombusviridae, and unclassified. Of them, eight viruses were novel species, belonging to Nairoviridae (Ji’an nairovirus and Yichun nairovirus), Phenuiviridae (Mudanjiang phlebovirus), Rhabdoviridae (Tahe rhabdovirus 1-3), Chuviridae (Yichun mivirus), and Tombusviridae (Yichun tombus-like virus), and five members were established human pathogens, including Alongshan virus, tick-borne encephalitis virus, Songling virus, Beiji nairovirus, and Nuomin virus. I. persulcatus ticks had significant higher viral species than those in H. japonica, H. concinna, and D. silvarum ticks. Significant differences in tick viromes were observed among Daxingan, Xiaoxingan and Changbai mountains. These findings showed an extensive diversity of RNA viruses in ticks in northeastern China, revealed potential public health threats from the emerging tick-borne viruses. Further studies are needed to explain the natural circulation and pathogenicity of these viruses.

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Detection of Jingmenviruses in Japan with Evidence of Vertical Transmission in Ticks

Cited by 27 publications

References 47 publications

Jingmen Tick Virus in Ticks from Kenya

Jingmen Tick Virus in Ticks from Kenya

Discovery of a Novel Jingmenvirus in Australian Sugarcane Soldier Fly (Inopus flavus) Larvae

Extensive diversity of RNA viruses in ticks revealed by metagenomics in northeastern China

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