Detection of Janus-activated kinase-1 and its interacting proteins by the method of luminescent oxygen channeling

Guo, Xinxin; Wu, Han-Tao; Zhuang, Si-Hui; Chen, Zhenhua; Liang, Rong-Liang; Yao, Chen; Wu, Yixuan; Liu, Tiancai

doi:10.1039/c6ra27424b

Cited by 2 publications

(1 citation statement)

References 29 publications

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“…However, they have many disadvantages, such as being time‐consuming and cumbersome operation. Compared with Co‐IP assay, homogeneous immunoassay has many advantages, such as less sample needed, less consuming time, simpler procedure, more samples detected, and high sensitivity [24‐27].…”

Section: Discussionmentioning

confidence: 99%

A homogeneous immunoassay for detection of the interaction between two tumor biomarkers of IGF1R‐β and SOCS1

Kuang

Deng

et al. 2020

Biotech and App Biochem

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The current protein interaction method is time consuming and cumbersome or the instrument is expensive. A new method that is convenient, fast, and high throughput needs to be studied urgently. The purpose of this study was to establish a homogeneous immunoassay to detect the interaction between insulin‐like growth factor‐1 receptor‐β (IGF1R‐β) and suppressor of cytokine signaling 1 (SOCS1). The recombinant vectors IGF1R‐β/pENTER and SOCS1/pENTER were constructed and transfected into 293T cells. Based on homogeneous immunoassay technology, we established a suitable method. The signal intensity in the 293T lysate that overexpressed IGF1R‐β and SOCS1, respectively, was compared with the signal intensity in the simultaneous expression of IGF1R‐β and SOCS1. The interaction between IGF1R‐β and SOCS1 was verified in vitro. The detection system for the interaction between IGF1R‐β and SOCS1 was established. Compared with other methods, homogeneous immunoassay has the advantages of being rapid and sensitive, having higher sensitivity, and easy to operate. The interaction between IGF1R‐β and SOCS1 was tested to verify the feasibility of this method and prove its practicability and sensitivity. This new method can be used as a high‐throughput platform for protein–protein interaction, with the advantages of trace detection, short detective time, and high detective sensitivity.

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Section: Discussionmentioning

confidence: 99%

A homogeneous immunoassay for detection of the interaction between two tumor biomarkers of IGF1R‐β and SOCS1

Kuang

Deng

et al. 2020

Biotech and App Biochem

Self Cite

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A highly water-stable dual-emission fluorescent probe based on Eu3+-loaded MOF for the simultaneous detection and quantification of Fe3+ and Al3+ in swine wastewater

Xia

Bao

Peng

et al. 2022

Analytica Chimica Acta

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Detection of Janus-activated kinase-1 and its interacting proteins by the method of luminescent oxygen channeling

Abstract: Janus-activated kinase-1 (JAK1) plays an important role in many signaling pathways, including the JAK–STAT and SOCS pathways.

Cited by 2 publications

References 29 publications

A homogeneous immunoassay for detection of the interaction between two tumor biomarkers of IGF1R‐β and SOCS1

A homogeneous immunoassay for detection of the interaction between two tumor biomarkers of IGF1R‐β and SOCS1

A highly water-stable dual-emission fluorescent probe based on Eu3+-loaded MOF for the simultaneous detection and quantification of Fe3+ and Al3+ in swine wastewater

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