Detection of intestinal bifidobacteria and lactobacilli in patients with Hirschsprung’s disease associated enterocolitis

Shen, Dihua; Shi, Cheng-ren; Chen, Jingjing; Yu, Shi-yao; Wu, Yan; Yan, Wenbo

doi:10.1007/s12519-009-0038-x

Cited by 42 publications

(31 citation statements)

References 22 publications

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“…Here, we noted a decrease in Lactobacillus in the EdnrB -null animals over time while the EdnrB -het animals demonstrated an increase (Figure 2). This result recapitulates that seen in human HSCR patients, who also display decreased levels of Lactobacillus [7]. In their animal model of HSCR, Ward, et.…”

Section: Discussionsupporting

confidence: 79%

“…The pathogenesis and etiology of HAEC have not been clearly determined although alterations in host innate immunity, intestinal barrier function and gut microflora have been proposed to contribute to HAEC susceptibility [3,4]. Clinical and animal investigations of HAEC have attempted to identify specific organisms that promote enterocolitis development [5–7]. A single, causative organism has not been isolated, but rather a handful of potential contributors, including Clostridium difficile , Escherichia coli , and viruses have been suggested.…”

Section: Introductionmentioning

confidence: 99%

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Intestinal dysbiosis and bacterial enteroinvasion in a murine model of Hirschsprung’s disease

Pierre

Barlow‐Anacker

Erickson

et al. 2014

Journal of Pediatric Surgery

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Background/Purpose Hirschsprung’s disease (HSCR), characterized by the absence of ganglia in the distal colon, results in functional obstruction. Despite surgical resection of the aganglionic segment, around 40% of patients suffer recurrent life threatening Hirschsprung’s-associated enterocolitis (HAEC). The aim of this study was to investigate whether gut microbiota and intestinal immunity changes contribute to the HAEC risk in a HSCR model. Methods Mice with neural crest conditional deletion of Endothelin receptor B (EdnrB) and their littermate controls were used (EdnrB-null and EdnrB-het). Bacterial DNA was prepared from cecal contents of P16–18 and P21–24 animals and pyrosequencing employed for microbiome analysis. Ileal tissue was isolated and secretory phospholipase A2 (sPLA2) expression and activity determined. Enteroinvasion of E. coli into ileal explants was measured using an ex vivo organ culture system. Results EdnrB-het and EdnrB-nulls displayed similar flora, sPLA2 expression and activity at P16–18. However, by P21–24, EdnrB-hets demonstrated increased Lactobacillus and decreased Bacteroides and Clostridium, while EdnrB-nulls exhibited reciprocal changes. EdnrB-nulls also showed reduced sPLA2 expression and luminal activity at this stage. Functionally, EdnrB-nulls were more susceptible to enteroinvasion with E. coli ex vivo and released less sPLA2 than EdnrB-hets. Conclusions Initially, EdnrB-het and EdnrB-nulls contain similar cecal flora but then undergo reciprocal changes. EdnrB-nulls display dysbiosis, demonstrate impaired mucosal defense, decreased luminal sPLA2 and increased enteroinvasion of E. coli just prior to robust colonic inflammation and death. These findings suggest a role for the intestinal microbiome in the development of HAEC.

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Section: Discussionsupporting

confidence: 79%

Section: Introductionmentioning

confidence: 99%

Intestinal dysbiosis and bacterial enteroinvasion in a murine model of Hirschsprung’s disease

Pierre

Barlow‐Anacker

Erickson

et al. 2014

Journal of Pediatric Surgery

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“…The greater relative increase in numbers of bifidobacteria and lactobacilli when their numbers are low in faeces indicates that the composition and numbers of bacteria in the intestines are balanced within particular ranges of bacterial numbers. Nonetheless, the above relationships suggest that responses of prebiotics might be better in stress, ageing and diseased conditions when the beneficial bacterial numbers decrease considerably (Hopkins et al, 2001;Burkholder et al, 2008;Shen et al, 2009). The relative increase of lactobacilli and bifidobacteria could be physiologically important by enhancing health status or reducing the risk of disease (Gibson et al, 2005).…”

Section: Microbial Counts and Serum Ig Concentrationsmentioning

confidence: 99%

Responses of feeding prebiotics on nutrient digestibility, faecal microbiota composition and short-chain fatty acid concentrations in dogs: a meta-analysis

Patra

2011

Animal

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The effects of prebiotics on digestibility, short-chain fatty acid (SCFA) concentrations and bacterial populations in the faeces and immunity in dogs were evaluated by meta-analyses. Overall, data from 15 published studies containing 65 different treatment means of 418 observations from different breeds of dogs were included in the data set. Feeding of prebiotics to dogs did not affect the nutrient intake (P . 0.10), nor did prebiotics change (P . 0.10) the digestibility of dry matter (DM) and fat. However, crude protein (CP) digestibility tended to decrease quadratically (P 5 0.06) with increasing dosages of prebiotics, although the degree of prediction was low (R 2 5 0.33). The concentration of total SCFA (P 5 0.08; R 2 5 0.90) tended to increase linearly, whereas concentration of acetate (R 2 5 0.25), propionate (R 2 5 0.88) and butyrate (R 2 5 0.85) increased quadratically with increasing dosage of prebiotics in the faeces of dogs. The numbers of beneficial bifidobacteria (P , 0.01; R 2 5 0.62) increased quadratically, but lactobacilli (P , 0.01; R 2 5 0.66) increased linearly with increasing supplementation of prebiotics. The changes in healthy bacterial numbers were affected by the interaction of initial bacterial numbers and dose of prebiotics; bacterial numbers increased relatively more when initial bacterial numbers were low. Dietary composition did not influence the response of prebiotics on lactobacilli and bifidobacterial numbers in this study. The numbers of pathogenic Clostridium perfringens and Escherichia coli were not affected by prebiotics. Prebiotics did not affect the serum immunoglobulin (Ig) concentrations such as IgG, IgA and IgM in dogs. Although prebiotics may tend to have an adverse effect on CP digestibility, prebiotics at doses up to 1.40% food intake (DM basis) might increase the beneficial bacterial populations and SCFA concentrations in the faeces of dogs. Thus, the feeding of prebiotics has a great prospective to improve the intestinal health of dogs.

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“…Так, D. H. Shen и соавт. в своем исследовании [10] сообщили о заметном уменьшении популяций Lactobacillus spp. и Bifidobacteria у детей c энтероколитом.…”

Section: обоснованиеunclassified

Optimizing Perioperative Antibacterial Therapy in Reconstructive Surgery for Colonic Agangliosis in Children

Sytkov¹,

Poddubny²,

Коzlov³

2016

Vopr. sovr. pediatr.

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Detection of intestinal bifidobacteria and lactobacilli in patients with Hirschsprung’s disease associated enterocolitis

Cited by 42 publications

References 22 publications

Intestinal dysbiosis and bacterial enteroinvasion in a murine model of Hirschsprung’s disease

Intestinal dysbiosis and bacterial enteroinvasion in a murine model of Hirschsprung’s disease

Responses of feeding prebiotics on nutrient digestibility, faecal microbiota composition and short-chain fatty acid concentrations in dogs: a meta-analysis

Optimizing Perioperative Antibacterial Therapy in Reconstructive Surgery for Colonic Agangliosis in Children

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