Detection of interspecific and intraspecific variation in Panicum millets through random amplified polymorphic DNA

Abstract

The potential use of random amplified polymorphic DNA (RAPD) was evaluated as a source of genetic markers for studying variation among four species of Panicum and within the crop species P. miliaceum and P. sumatrense. Polymorphism in RAPD markers was observed across and within species. The four species were distinct in RAPD patterns and were separated at low correlation values even with small samples involving single genotypes per species. Accessions of P. miliaceum were grouped according to geographical regions of origin. The study demonstrated that unlike isozyme and protein electrophoresis patterns, RAPD markers can be applied to studying genetic diversity, defining gene pools, and identifying cultivars for this group of millets.

Morphological and molecular analysis of common millet (P. miliaceum) cultivars compared to an aDNA sample from the 15th century (Hungary)

“…In contrast, RAPD polymorphism clustered common millets accessions according to geographical regions of origin, which indicated that genome of common millet was fairly plastic with potential for relatively rapid adaptation period during microevolution (M'Ribu & Hilu, 1994). An olive-black seeded biotype called 'wild proso millet' which appeared in North America 1970, after about 400 years of millet cultivation (as millet was introduced to North America in the 17th century) also indicated a plastic common millet genome (Strand & Behrens, 1981).…”

Development and Characterization of SSR Markers in Proso Millet Based on Switchgrass Genomics

“…A very limited number of DNA markers are reported in proso millet. A few RAPD and AFLP markers were reported in proso millet for genetic diversity analysis [10][11][12]. Among all PCR-based markers, microsatellites or simple sequence repeat (SSR) markers are the most informative and useful because they are co-dominant, multi-allelic, highly reproducible, abundant and are evenly distributed in the plant genome [13,14].…”

Development of Species-Specific Microsatellite Markers for Broomcorn Millet (Panicum miliaceum L.) via High-Throughput Sequencing

“…For broomcorn millet, isozymes and protein electrophoresis for intra-species grouping or classification have not been successful [1,47]. Genomic SSR markers in broomcorn millet were also identified more efficiently than the transferred SSR markers which selected from other crops.…”

“…This study was supported by the National Natural Science Foundation of China (Grant No.31301386), the National Millet Crops Research and Development System (NMCRDS), China Agriculture Research System (CARS-07-12 [1].5-A1) and the Agricultural Science and Technology Innovation Program (ASTIP) in CAAS. We are grateful to Dr. Dahai Wang and Liping Sun (Beijing Autolab Biotechnology Co., Ltd) for special contribution to this work.…”

“…Broomcorn millet (Panicum miliaceum L, 2n=4x=36), an important member of the genus Panicum [1] was domesticated in China more than 10,000 year [2,3] and it is an outstanding crop for high adaptability to climate change, especially abiotic stresses, such as drought, salinity and infertility [4][5][6]. The growth duration of broomcorn millet is 60-90 days [7], which is the shortest in crops and it is usually grown for exploration of new wastelands and deserts or as a remediation crop in the event of natural disasters [8,9].…”