Detection of interspecies idiotypic cross‐reactions associated with antibodies to hepatitis B surface antigen

Kennedy, Ronald C.; Ionescu-Matiu, Irina; Sanchez, Yanuario; Dreesman, Gordon R.

doi:10.1002/eji.1830130310

Cited by 68 publications

(35 citation statements)

References 18 publications

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“…The potential usefulness of anti-id for antigenspecific immunosuppression is limited by the need to produce specific, anti-id antisera for each patient. Our results demonstrating crossreactivity remove this limitation, and are in concordance with findings in such diverse antigen systems as Hepatitis B (42), rheumatoid factor (43), p-azophenylarsonate (44), and more recent studies with acetylcholine receptor (45). The findings of crossreactivity and an anti-id that conformationally appears to be the "internal image" of antigen places this anti-id in the second class of a recently proposed system for categorizing anti-ids (46,47), and implies a common, antigenic determinant for the Rbabs binding site.…”

Section: Discussionsupporting

confidence: 90%

Production, isolation, and characterization of rabbit anti-idiotypic antibodies directed against human antithyrotrophin receptor antibodies.

Baker¹,

Lukes²,

Burman³

1984

J. Clin. Invest.

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A s bstract. Previous studies have shown that antiidiotypic antibodies can be developed in vivo through animal immunization with idiotype, and that these antibodies can be isolated from other anti-immunoglobulin antibodies by affinity purification. These techniques have relied on large amounts ofidiotype, which were produced either by hyperimmunization or by monoclonal antibodies, to serve as the affinity adsorbent. In the present study, we produced anti-idiotypic antibodies to human anti-thyroid-stimulating hormone (TSH) receptor antibodies by first injecting rabbits with (TSH receptor purified) IgG from Graves' patients. The resulting antiserum was then adsorbed with Sepharose-coupled TSH in an attempt to specifically bind and isolate the anti-idiotype. The antibody obtained from this process was shown to bind specifically to TSH receptor-binding antibodies from Graves' patients, and this binding could be inhibited by 56% with the addition of 10-4 M TSH but not by HCG (10-2 M). The anti-idiotype also bound to TSH, and this binding could be specifically inhibited by receptor-purified Graves' IgG (60% inhibition at 10 Ag/ml IgG), but not by IgG from normal subjects (no inhibition at 50 ,g/ml IgG). In a TSH receptor binding assay, the anti-idiotype could inhibit TSH receptor binding in Graves' sera at a 1,000-fold lower concentration than could anti-kappa/

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Section: Discussionsupporting

confidence: 90%

Production, isolation, and characterization of rabbit anti-idiotypic antibodies directed against human antithyrotrophin receptor antibodies.

Baker¹,

Lukes²,

Burman³

1984

J. Clin. Invest.

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“…For example, four rabbit antisera raised against two different human IgG anti-hepatitis B virus surface antigen (HBs) preparations detected a common determinant shared by three anti-HBs preparations, and anti-HBs-positive sera obtained from six haemophiliac patients. This anti-HBs idiotype was not found on IgG isolated from sera negative for anti-HBs (Kennedy & Dreesman, 1983). Similar data have been reported in other cross-reactive idiotypic systems (Zouali et al, 1984a, b).…”

Section: Discussionsupporting

confidence: 89%

“…Whereas the human immune response to HBs is idiotypically restricted (Kennedy & Dreesman, 1983), we found that anti-gpl20 antibodies of HIV-l-infected individuals express unrelated idiotypic determinants. These idiotypic features of the humoral immune responses against the hepatitis B virus (HBV) and HIV, which are associated with 'natural' infection of man, may reflect the genomic heterogeneity of HBV and HIV.…”

Section: Discussionmentioning

confidence: 66%

Idiotypic Expression of Anti-gp120 Antibodies in Unrelated Human Immunodeficiency Virus-infected Individuals

Février¹,

Boudet²,

Corre³

et al. 1992

Journal of General Virology

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Although it is recognized that human immunodeficiency virus (HIV) env genes exhibit a high degree of variability, little is known about the molecular heterogeneity of gp 120-specific antibodies in infected individuals. As a first step to approach this issue, we investigated the idiotypic relatedness of anti-gpl20 antibodies present in the serum of HIV-infected individuals. Idiotypic determinants (idiotopes) are fingerprints of the variable region of the antibody molecule and, as such, they represent unique probes with which to explore the diversity of the immune response. We isolated IgG anti-gp120 antibodies from the serum ofa seropositive asymptomatic individual by affinity chromatography. The purified antibodies were shown to bind gpl20 and gpl60 by ELISA, Western blotting and radio-immunoprecipitation. They also recognized HIV-infected human T cells as detected by immunofluorescence. Anti-idiotypic reagents were generated against this gp 120 idiotype, and one of them was used to study anti-gp120 idiotypic diversity in a panel of 65 sera drawn from AIDS and AIDS-related complex patients, and from HIV seropositive asymptomatic individuals. Sixty normal human sera were used as negative controls. We found no evidence for common idiotopes on anti-gpl20 antibodies of unrelated individuals. In contrast, we also noticed that the idiotypic profile expressed sequentially at two different intervals in a persistently infected individual showed little variation. Finally, when the diversity of murine anti-gp 120 antibodies with a monoclonal antiidiotype was analysed, no evidence of cross-reactive idiotopes in the murine system was found.

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“…Thus, idiotypic sharing has been found among antibodies raised in goats to human hemoglobins (13), and human antibodies to hepatitis B surface antigen ( 14). Similarly, autoantibodies to rheumatoid factors (15), anti-acetylcholine receptor (16) antibodies, antithyroglobulin antibodies (17), and DNA antibodies (7) have all been shown to share idiotypic determinants: These observations indicate restrictions in the repertoire of variable region immunoglobulin genes, and imply that major cross-reactive idiotypic families are the products of common germ line genes dispersed throughout the population rather than the result of somatic mutations.…”

Section: Discussionmentioning

confidence: 99%

Detection of cross-reactive anti-DNA antibody idiotypes on renal tissue-bound immunoglobulins from lupus patients.

Isenberg¹,

Collins²

1985

J. Clin. Invest.

129

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Cross-reactive anti-DNA antibody idiotypes have been identified on tissue-bound immunoglobulins in a study of renal biopsies from 26 systemic lupus erythematosus (SLE) patients. 12 (46%) biopsies were shown to have one or both the idiotypes tested for by anti-idiotypic reagents. The idiotypes were identified in the glomerular basement membrane, the mesangial cell cytoplasm, and in focal tuft proliferations. In contrast, in none of 24 immunoglobulin-positive disease control biopsies could either idiotype be demonstrated. Blocking studies in two patients indicated that the idiotypes were on anti-DNA antibodies. These findings indicate that some tissue-bound autoantibodies are derived from related families of high-frequency germ line genes that are expressed in SLE patients. The potential role of anti-idiotypic therapy in SLE is discussed.

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Detection of interspecies idiotypic cross‐reactions associated with antibodies to hepatitis B surface antigen

Cited by 68 publications

References 18 publications

Production, isolation, and characterization of rabbit anti-idiotypic antibodies directed against human antithyrotrophin receptor antibodies.

Production, isolation, and characterization of rabbit anti-idiotypic antibodies directed against human antithyrotrophin receptor antibodies.

Idiotypic Expression of Anti-gp120 Antibodies in Unrelated Human Immunodeficiency Virus-infected Individuals

Detection of cross-reactive anti-DNA antibody idiotypes on renal tissue-bound immunoglobulins from lupus patients.

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