Detection of interleukin-6, interleukin-8, and interleukin-11 in plasma from women with spontaneous abortion

Koumantaki, Yvonni; Matalliotakis, Ioannis; Sifakis, Stavros; Kyriakou, Despina; Neonaki, M; Goymenou, Anastasia; Koumantakis, E.

doi:10.1016/s0301-2115(01)00289-5

Cited by 46 publications

(31 citation statements)

References 18 publications

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“…When comparing interleukin levels, contrary to prior reports (18,(21)(22)(23), we found no difference in serum IL-6 or IL-2R among the three groups. In contrast, on day 28, the median IL-8 levels were found to be statistically significantly lower in the ectopic gestation group when compared with all intrauterine pregnancies combined (P<.04).…”

Section: Patient Characteristics Are Shown Incontrasting

confidence: 99%

Early serum interleukin-8 evaluation may prove useful in localizing abnormally implanted human gestations after in vitro fertilization

Morelli

Keegan

Krey

et al. 2008

Fertility and Sterility

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Section: Patient Characteristics Are Shown Incontrasting

confidence: 99%

Early serum interleukin-8 evaluation may prove useful in localizing abnormally implanted human gestations after in vitro fertilization

Morelli

Keegan

Krey

et al. 2008

Fertility and Sterility

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“…Ultrasound studies have observed the interval between occurrence of fetal loss and its detection to be up to several weeks (45,46). Thus, interpretation of studies that have not collected samples from women prior to experiencing miscarriage is uncertain (11,(19)(20)(21)37,38,46). We conducted analyses by time between sample collection and diagnosis to help address this concern.…”

Section: Discussionmentioning

confidence: 99%

“…Previous studies suggesting altered cytokine production in miscarriage have included small study populations, evaluated few factors, and/or utilized samples collected at diagnosis of miscarriage, subsequent to fetal demise (5,11,(18)(19)(20)(21)(22)(23). Given the ambiguous findings regarding the association among Th1 and Th2 cytokines and miscarriage, the complex biological interplay between these factors, and the lack of information regarding the relation between TPO and miscarriage, we evaluated the hypothesis that cytokine levels are altered in miscarriage using a large number of serum samples collected prior to report of miscarriage for simultaneous assessment of multiple cytokines.…”

Section: Introductionmentioning

confidence: 99%

Circulating levels of cytokines during pregnancy: thrombopoietin is elevated in miscarriage

Whitcomb

Schisterman²,

Klebanoff³

et al. 2008

Fertility and Sterility

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Objective-To evaluate the hypothesis that cytokine levels are associated with miscarriage risk using serum samples collected prior to report of miscarriage.Design-A nested case-control study.Setting-Biospecimens from the multi-site Collaborative Perinatal Project, University of Florida laboratory assessment of IL-1ra, IL-1β, IL-4, IL-6, IFN-γ, TNF-α, TPO and G-CSF.Patients-were obtained from cases of miscarriage (N=439) matched to controls (N=373) by gestational age (GA) at sample collection. Intervention-None. Main outcome measures-,Miscarriage.Results-Increased risk of miscarriage was associated with elevated TPO (AOR: 1.16, 95% confidence interval (CI):1.00 -1.36) and decreased G-CSF (AOR: 0.78, 95% CI 0.64 -0.95). When analysis was restricted to samples collected more than 35 days prior to miscarriage, the effect of G-CSF was not observed (AOR: 0.96, 95% CI 0.72 -1.28), whereas increased risk related to higher TPO remained.Conclusions-Circulating levels of TPO may be associated with increased risk of miscarriage.

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“…There is evidence of a diminished Th2 immune response to placental antigens in women with RLP (5). Plasma levels of IL-6, IL-8 and IL-11 have been found to be decreased in such women compared with normal pregnancies (6).…”

Section: Introductionmentioning

confidence: 99%

Single nucleotide polymorphisms in the promoter region of the interleukin-6 gene and the risk of recurrent pregnancy loss in japanese women

Saijo

Sata

Yamada

et al. 2004

Fertility and Sterility

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Y Saijo et al. 17994-revised CapsuleThere was a statistically significant difference in the IL-6-634C→G genotype frequency between Japanese patients with recurrent pregnancy loss (RPL) and controls.Patients who carry the -634 G allele may have a decreased risk of RPL. Y Saijo et al. 17994-revised Structured AbstractObjective: To investigate the relationships between recurrent pregnancy loss (RPL) and single nucleotide polymorphisms (-634 C→G and -174 G→C genotypes) in the promoter region of the IL-6 gene in the Japanese population.Design: A case-control study. Setting: Department of Obstetrics and Gynecology in a university hospital.Patient(s): 76 cases with RPL. Controls were 93 fertile women. Intervention(s):Frequency and distribution of the promoter region of the IL-6 gene allele. Main Outcome Measure(s): Determination of IL-6 promoter gene polymorphisms was performed by polymerase chain reaction and gel electrophoresisResults: There was a significant difference in the -634C→G genotype frequency (CC vs. CG/GG) between the women with RPL and the controls. The risk of RPL was lower in the carrier of the G allele than in women with the wild type（CC）, with OR = 0.46 (95% CI = 0.24-0.91). On the other hand, we did not detect any carrier of -174C in the 169 subjects. Conclusion(s):The results suggest that women carrying the -634 G allele of the IL-6 gene may have a decreased risk of RPL in the Japanese population. A shift to type-2 T-helper (Th2) cytokine production with abundant interleukine (IL)-6 and IL-10 is considered essential for the maintenance of normal pregnancy. There is evidence of a diminished Th2 immune response to placental antigens in women with RLP (5). Plasma levels of IL-6, IL-8 and IL-11 have been found to be decreased in such women compared with normal pregnancies (6).Additionally, IL-6 levels in maternal serum (7), amniotic fluid (8), vaginal fluid (9), and placenta (10) have been found to increase during the process of normal labor compared to the nonlabor state.One study demonstrated an increase in the frequencies of type-1 T-helper (Th1) cytokine IL-1β gene promoter region variants IL-1B-511C and IL-1B-31T in RPL women with RLP (11). One of the Th2 cytokines, IL-10 promoter region variant (-1082G→A) was not associated with RPL (12,13). However, relationships between many diseases and IL-6 promoter gene polymorphisms, such as IL-6 -174G→C (14, 15) and IL-6 -634C→G (16), were recently demonstrated. It is also known that the former polymorphism is frequently found in Caucasians (14, 15) and the latter in Japanese (16). There have been no reports of other frequent polymorphisms of the IL-6 gene, and the relationship between any IL-6 gene polymorphism and RPL has not yet been investigated. In the present study, therefore, we assessed these two promoter polymorphisms and investigated whether either of these IL-6 gene polymorphisms Materials and MethodsThis case-control study was performed in Sapporo, Japan, during the years 1999-2002. We studied 76 cases aged 20-42 years with a history of RP...

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Detection of interleukin-6, interleukin-8, and interleukin-11 in plasma from women with spontaneous abortion

Cited by 46 publications

References 18 publications

Early serum interleukin-8 evaluation may prove useful in localizing abnormally implanted human gestations after in vitro fertilization

Early serum interleukin-8 evaluation may prove useful in localizing abnormally implanted human gestations after in vitro fertilization

Circulating levels of cytokines during pregnancy: thrombopoietin is elevated in miscarriage

Single nucleotide polymorphisms in the promoter region of the interleukin-6 gene and the risk of recurrent pregnancy loss in japanese women

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