Detection of interleukin‐6 in human dental pulp and periapical lesions

Abstract

Pulpal and periapical diseases are characterized by inflammation. The cytokine IL-6 is a major mediator of the host response to tissue injury and infection. This study examined the level of interleukin-6 (IL-6) in six inflamed human pulps and six human periapical lesions of endodontic origin using ELISA. Pulp samples from eight clinically impacted teeth were used as controls. The periapical samples exhibited significant levels of IL-6 (mean = 78.1 +/- 9 pg/mg protein) as did inflamed pulpal tissues mean = 36 +/- 3.9 pg/mg protein) compared to healthy pulp mean = 0.01 +/- 0.02 pg/mg protein). These data indicated that IL-6 was produced and released locally in the inflamed pulpal and periapical lesions.

Interleukin-6 and interleukin-10 in experimentally induced rat pulpal inflammation

“…Matsushima et al demonstrated that Gram-negative bacteria, such as L. casei, from carious lesions, might be involved in developing pulpitis through the stimulation of IL-6 production 17) . The results of this study were comparable to the study of Barkhordar' s 5) , which examined the level of interleukin-6 in inflamed human dental pulps and periapical lesion. In their study, the inflamed pulpal tissues exhibited significantly higher levels of IL-6 (mean = 36±3.9pg/mg protein) compared to healthy pulp.…”

“…In one study on periodontal disease, the amount of IL-6 and IL-10 were significantly higher in the inflamed gingival tissues than in the peripheral blood from the healthy subjects 6) . IL-6 was detected in human pulps, periapical lesions 5) and odontogenic cysts 7) . And it has been shown that the production of IL-6 was stimulated by Prevotella intermedia lipop-olysaccharide 8) .…”

“…The occurrence of interleukin(IL)-1 and IL-1 producing cells has been demonstrated in human inflamed pulps 3) . The roles of IL-2 and IL-6 have also been studied in healthy and inflamed dental pulps 4,5) . In one study on periodontal disease, the amount of IL-6 and IL-10 were significantly higher in the inflamed gingival tissues than in the peripheral blood from the healthy subjects 6) .…”

GP130 cytokines and bone remodelling in health and disease

“…In vitro studies have reported that treatment of healthy human periodontal ligament cells with lipopolysaccharide (LPS) from inflammatory pathogens involved in perio-http://bmbreports.org BMB reports dontal disease stimulates IL-6 release (56). Local expression of IL-6 at periodontic sites is higher than levels in similar sites in healthy controls (57,58). Surprisingly however, periodontal lesions induced by pulp exposure were larger in IL-6 null mice than wild type, but this model does not involve infection (59).…”

Pannexin3 inhibits TNF‐α‐induced inflammatory response by suppressing NF‐κB signalling pathway in human dental pulp cells

“…It has been established that the expression of IL‐1β inflamed pulp fibroblasts is 2.5‐fold higher than that in healthy pulp fibroblasts 39. Similarly, the level of IL‐6 in diseased human dental pulp tissues was dramatically increased compared with normal pulps 40. These findings demonstrated that those cytokines might be involved in pulpal inflammation 41.…”