Detection of Interleukin 6 (IL-6) antigen in the head and neck carcinoma cells

Abstract

Background: Interleukin 6 (IL-6) is a pleiotropic cytokine produced not only by inflammatory cells, but also by the squamous epithelium of oral cavity, tongue and/ or laryngeal mucosa (probably explaining its presence in saliva and respiratory secretions).Methods: Immunohistochemical staining for IL-6 antigen was performed in histological sections coming from 98 head and neck squamous cell carcinoma (HNSCC) samples using monospecific IL-6 antibody (rabbit anti-IL-6 serum purchased from Abcam, product Ab6672) and taking advantage of the BenchMark Ultra IHC/ ISH staining module (Ventana Discovery XT automated system, Tuscon, USA). Results:The expression of IL-6 antigen was examined in a total of the 98 head and neck squamous cell carcinoma (HNSCC) samples, i.e. 33 laryngeal, 55 tongue and 10 nasal cavity specimens, along with 18 relevant normal tissue samples (negative controls) and, in addition, in 10 non-HNSCC tumours (alternative tumour controls). Immuhistochemical staining confirmed the presence of IL-6 antigen in the cytoplasm of carcinoma cells as well as in the stroma of all the HNSCC tumours (100%). In conventional squamous carcinomas a rather confluent staining of IL-6 could be seen in the cytoplasm of dysplastic prickle cells, while in anaplastic cells of undifferentiated carcinomas a less intensive fine granular staining pattern dominated. For comparison, occasional and less intensive staining for the IL-6 antigen was seen in 5 out of 10 alternative carcinomas (50%) and/or in 10 out of 16 (60%) relevant normal tissue controls. Conclusion:We confirmed the widespread expression IL-6 antigen as detected by immunohistochemical staining in all the HNSCC samples examined (100% positive), regardless whether using purchased slides or sections prepared from our domestic biopsy material. A proportion (60 %) of the normal laryngeal and mouth cavity epithelium was positive as well, though in the letter, the frequency and intensity of antigen expression was considerably lower.

An interdigitated aptasensor to detect interleukin‐6 for diagnosing rheumatoid arthritis in serum

“…These variations reflected the difference (increase/decrease) in the results of the current measurements. The current strategy worked well for IL-6 detection and is comparable to other currently available established sensing systems [26,27] (Table 1).…”

Evaluation of Salivary Interleukin-6 in Patients with Oral Squamous Cell Carcinoma, Oral Potentially Malignant Disorders, Chronic Periodontitis and in Healthy Controls - A Cross-Sectional Comparative Study

“…[ 13 ] The literature reveals various studies on the diagnostic role of IL-6 in malignancy. [ 14 ] Saliva is regarded as a potential alternative to blood serum and urine and to be a non-invasive technique. [ 15 ] In our study, we have attempted to enlighten the characteristic feature of salivary IL-6 in OSCC, OPMDs and CP as compared with healthy controls.…”

Ellagic acid regulates hyperglycemic state through modulation of pancreatic IL-6 and TNF- α immunoexpression

“…The following immunohistochemical assays were performed, using an avidin biotin complex (ABC) technique in all cases (Elite–ABC, Vector Laboratories, CA, USA) in order to identify antibody staining for the following: (i) proliferating cell nuclear antigen (PCNA) (antibody dilution 1:100; DAKO, Japan Co., Tokyo, Japan) Tousson et al ( Tousson et al, 2011 ); (ii) insulin (antibody dilution 1:100; Monoclonal Anti-insulin, No. I2018, Sigma) Suarsana et al ( Suarsana et al, 2016 ); (iii) TNF-α (antibody dilution 1:200; DAKO Japan Co, Tokyo, Japan) El-Masry et al ( El-Masry et al, 2020a ) and (iv) IL-6 (antibody dilution 1:200; rabbit anti-IL-6 serum, Abcam, product Ab6672) Rajčáni et al ( Rajčáni et al, 2019 ).…”