Detection of interaction between biomineralising proteins and calcium carbonate microcrystals

Rademaker, Hanna; Launspach, Malte

doi:10.3762/bjnano.2.26

Cited by 7 publications

(3 citation statements)

References 8 publications

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“…Distinct diffraction peaks at 26°, 27°, 33°, 36°, 38°, 41°, 43°, 46°, 48° and 50° confirmed the aragonite type of biocement crystals 20 . A sharp peak at 2θ = 22º in diffraction pattern indicates the presence of silicates in the biocement 21,22 . Different bacterial species induce different polymorphs of CaCO 3 crystals.…”

Section: Production Of Biocement From Tap Water Using Bacillus Cereusmentioning

confidence: 99%

Bacillus cereus KLUVAA Mediated Biocement Production Using Hard Water and Urea

Anitha

Abinaya

Prakash

et al. 2018

Chem.Biochem.Eng.Q.

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In the present study, a potential bacterial strain with maximum urease activity was isolated from urea-rich paddy field soil for biocement production. The bacterial isolate was screened using Christensen selective agar media and named as KLUVAA. This isolate was found to be tolerant up to 10 % urea. 16S rRNA sequencing analysis identified the isolate KLUVAA as Bacillus cereus. Biocement production was carried out using tap water with 431.7 mg L-1 of hardness as a natural source of calcium. Functional groups present in biocement were analysed using FT-IR spectrum. The morphology and elemental composition of the biocement was studied using SEM with EDS mapping and XRD analyses. Thermogravimetric analysis was used to study the thermal stability of the microbial biocement. Further, process parameters were optimized for enhancing the yield of biocement.

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Section: Production Of Biocement From Tap Water Using Bacillus Cereusmentioning

confidence: 99%

Bacillus cereus KLUVAA Mediated Biocement Production Using Hard Water and Urea

Anitha

Abinaya

Prakash

et al. 2018

Chem.Biochem.Eng.Q.

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“…Together with other proteins of the organic matrix, one function proposed for Perlucin is the nucleation of aragonite crystals [13] , [32] by binding to aragonite instead of calcite, [33] . In calcium carbonate precipitation experiments, native Perlucin clearly leads to faster precipitation of CaCO 3 [13] .…”

Section: Introductionmentioning

confidence: 99%

Splice Variants of Perlucin from Haliotis laevigata Modulate the Crystallisation of CaCO3

et al. 2014

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Perlucin is one of the proteins of the organic matrix of nacre (mother of pearl) playing an important role in biomineralisation. This nacreous layer can be predominately found in the mollusc lineages and is most intensively studied as a compound of the shell of the marine Australian abalone Haliotis laevigata. A more detailed analysis of Perlucin will elucidate some of the still unknown processes in the complex interplay of the organic/inorganic compounds involved in the formation of nacre as a very interesting composite material not only from a life science-based point of view. Within this study we discovered three unknown Perlucin splice variants of the Australian abalone H. laevigata. The amplified cDNAs vary from 562 to 815 base pairs and the resulting translation products differ predominantly in the absence or presence of a varying number of a 10 mer peptide C-terminal repeat. The splice variants could further be confirmed by matrix-assisted laser desorption ionisation time of flight mass spectrometry (MALDI-ToF MS) analysis as endogenous Perlucin, purified from decalcified abalone shell. Interestingly, we observed that the different variants expressed as maltose-binding protein (MBP) fusion proteins in E. coli showed strong differences in their influence on precipitating CaCO3 and that these differences might be due to a splice variant-specific formation of large protein aggregates influenced by the number of the 10 mer peptide repeats. Our results are evidence for a more complex situation with respect to Perlucin functional regulation by demonstrating that Perlucin splice variants modulate the crystallisation of calcium carbonate. The identification of differentially behaving Perlucin variants may open a completely new perspective for the field of nacre biomineralisation.

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“…Indeed, it has been reported that aragonite has a surface area almost ten times higher than that of calcite, justifying a higher adsorption efficiency for mass unit of CaCO3. 27 The experimental data indicate that the amount of dye entrapped within calcium carbonate increases increasing its concentration solution. On the other side, the percentage of dye removed from the solution during the crystallization process decreases with its concentration.…”

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confidence: 98%

Insights on the interaction of calcein with calcium carbonate and its implications in biomineralization studies

et al. 2018

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The effects of calcein, a marker commonly used to assess mineral growth in calcifying organism, on calcite and aragonite structure have been investigated. Calcein is entrapped within calcite and aragonite and modifies the shape and morphology of both polymorphs. Moreover, in the presence of Mg 2+ , it inhibits aragonite formation in favor of magnesium calcite.Studying and understanding of the biomineralization processes employ various methods to mark the growth of the inorganic components of the organisms, allowing researchers to correlate a particular region of the skeleton to the instant of its deposition. A common method is to use fluorescent molecules to label a particular stage of the deposition process. 1 Calcein, a strongly fluorescent organic dye synthetized via modification of fluorescein with ethylenediaminetetraacetic acid analogues, 2 has been widely used to study the growth of calcified skeletons because it coordinates calcium ions and is entrapped within the growing inorganic matrix. 3-5 Calcein has been successfully applied to mark bone growth 6 as well as shell and coral skeleton formation. 1,7 However, even if the transport of this dye inside living organisms was deeply characterized, 3,8 no information on the effect of calcein on the morphology and polymorphism of calcium carbonate crystals is readily available. The effect of soluble additives, both synthetic and biogenic, on calcium carbonate crystals has been extensively studied to understand biomineralization 9-13 and to fabricate novel advanced materials [14][15][16][17][18][19][20] . Based on these studies, models on the mechanisms of interaction between various additives and the growing mineral have been proposed. Interaction and entrapment of calcein within the crystal structure of calcium carbonates is known to occur due its chemical structure. 21,22 In this work, we examined in vitro the effect of calcein on the growth of calcite and aragonite crystals, the two main polymorphs of CaCO3 found in living organisms, using the calcein concentration in the range usually adopted for in vivo labelling. For this goal, we used the vapor diffusion method, consisting in the diffusion of NH3(g) and CO2(g) obtained from the decomposition of (NH4)2CO3(s), into a Ca 2+ solution containing the dye. This method is relevant for biomineralization process due to the slow increase of the concentration of CO3 2ions in the crystallization solution. It exploits carbonate speciation in water resembling the process suggested to occur in living systems, in which carbonic anhydrase controls the supply of carbonate to the calcification site. 23 In our experimental setting, calcite precipitated when solely Ca 2+ was present in the crystallization solution, while aragonite was the main polymorph obtained when Mg 2+ is co-present with a Mg 2+ /Ca 2+ molar ratio equal to 4. 24,25 No detectable inhibition or promotion of precipitation due to the presence of calcein was observed, as evaluated by the measure of total Ca 2+ deposited in each experiment (see table SI1). To ...

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Detection of interaction between biomineralising proteins and calcium carbonate microcrystals

Cited by 7 publications

References 8 publications

Bacillus cereus KLUVAA Mediated Biocement Production Using Hard Water and Urea

Bacillus cereus KLUVAA Mediated Biocement Production Using Hard Water and Urea

Splice Variants of Perlucin from Haliotis laevigata Modulate the Crystallisation of CaCO3

Insights on the interaction of calcein with calcium carbonate and its implications in biomineralization studies

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