Detection of Integrons and Staphylococcal Cassette Chromosome mec Types in Clinical Methicillin-resistant Coagulase Negative Staphylococci Strains

Hajiahmadi, Fahimeh; Ghale, Elham Salimi; Alikhani, Mohammad Yousef; Mordadi, Alireza; Arabestani, Mohammad Reza

doi:10.24171/j.phrp.2017.8.1.06

Cited by 3 publications

(8 citation statements)

References 34 publications

(31 reference statements)

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“…The rate of methicillin resistance in our study was 48.8% that was close to Namvar et al [19] and Hajiahmadi et al [23] results with 47.2% and 50%, respectively. In our study, the most common MR-CoNS isolates belonged to both of S. epidermidis and S. haemolyticus (45.45%) which was consistent with previous reports from Iran and other countries [3,23,24,25]. One of the species that so far has not been reported from other regions of Iran, but was isolated in Ahvaz city, was S. petrasii.…”

Section: Discussionsupporting

confidence: 90%

“…The prevalence of MR-CoNS in clinical samples has been reported between 47% and 91% in different cities of Iran [19,20,21,22]. The rate of methicillin resistance in our study was 48.8% that was close to Namvar et al [19] and Hajiahmadi et al [23] results with 47.2% and 50%, respectively. In our study, the most common MR-CoNS isolates belonged to both of S. epidermidis and S. haemolyticus (45.45%) which was consistent with previous reports from Iran and other countries [3,23,24,25].…”

Section: Discussionsupporting

confidence: 86%

“…This could be because of different protocols and panels of antibiotics used in various hospitals. Although in our study the lowest resistance was observed to linezolid (22.7%), this rate of resistance was high in comparison with many studies in Iran and other countries [23,28,29,30]. According to multinational and multicenter surveillance studies, more than 99% of coagulase-negative staphylococci and S. aureus clinical strains are sensitive to linezolid (31) [31].…”

Section: Discussioncontrasting

confidence: 51%

“…The SCCmec is a mobile genetic element widely distributed among MR-CoNS species that varies depending on the host species, various environments and geographical locations [16]. In the present study, the most abundant SCCmec type among MR-CoNS was type I (45.5%), whereas in other studies in Iran SCCmec type IV, III and V were more frequent [20,23,28]. It isn't easy to compare our results with other results in Iran because in many studies the diversity of SCCmec type has been evaluated only among S. epidermidis [19,23,28].…”

Section: Discussionsupporting

confidence: 40%

“…In the present investigation, the susceptibility patterns of MR-CoNS were at variance with some other studies from Iran. For example in Hajiahmadi et al [23] and Havaei et al [27] studies the highest resistance was observed to trimethoprim-sulfamethoxazole (56%) and ciprofloxacin (65%) respectively. This could be because of different protocols and panels of antibiotics used in various hospitals.…”

Section: Discussionmentioning

confidence: 99%

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Investigation of SCCmec types I–IV in clinical isolates of methicillin-resistant coagulase-negative staphylococci in Ahvaz, Southwest Iran

et al. 2020

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Today methicillin resistant coagulase-negative staphylococci (MR-CoNS) are important in terms of causing significant nosocomial infections. Besides, MR-CoNS are confirmed as the reservoir of SCCmec elements that carry mecA (methicillin-resistant) gene. Hence, the present study was designed to evaluate the susceptibility pattern, prevalence and diversity of SCCmec types I, II, III, and IV in MR-CoNS strains. In this cross-sectional study, 44 clinical isolates of MR-CoNS were identified using the cefoxitin disc method and further confirmation by polymerase chain reaction (PCR) amplification of the mecA gene. Antimicrobial susceptibility of isolates was investigated by disc diffusion. The identification of CoNS was done by amplification and sequencing of the tuf gene. Multiplex PCR method was done for the determination of SCCmec types. In the present study, the Staphylococcus epidermidis and Staphylococcus haemolyticus were the most predominant isolates with a prevalence of 45.4%. The highest resistance rates were observed against erythromycin (84.1%) and clindamycin (75%). Multiplex PCR revealed the SCCmec type I as the predominant type in the present study. Our study showed that there was no significant relationship between the presence of different types of SCCmec elements and resistance to antibiotics. The present study highlighted a frequent prevalence of MR-CoNS harboring SCCmec type genes in Ahvaz, southwest of Iran. Thus, the molecular typing and periodical monitoring of their drug resistance pattern should be considered in national stewardship programs to designing useful antibiotic prescription strategies.

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Section: Discussionsupporting

confidence: 90%

Section: Discussionsupporting

confidence: 86%

Section: Discussioncontrasting

confidence: 51%

Section: Discussionsupporting

confidence: 40%

Section: Discussionmentioning

confidence: 99%

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Investigation of SCCmec types I–IV in clinical isolates of methicillin-resistant coagulase-negative staphylococci in Ahvaz, Southwest Iran

et al. 2020

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Characterization and molecular evaluation of Staphylococcus aureus isolated from poultry and dairy cattle milk in Iraq

Almousawi¹,

Alhatami

Neama

et al. 2022

3rd International Scientific Conference of Alkafeel University (Iscku 2021)

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Prevalence and Potential Risk Factors for the Acquisition of Antibiotic‐Resistant Staphylococcus spp. Bacteria Among Pastoralist Farmers in Kajiado Central Subcounty, Kenya

Moraa

Kagira

Maina

et al. 2023

BioMed Research International

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Antimicrobial resistance (AMR) is a growing health problem globally. To address this challenge, there is a need to generate baseline data on the prevalence and AMR profile of the main disease-causing bacteria. Here, we interrogated the prevalence of bacteria in the nasal cavity of healthy pastoralists in Kajiado Central Subcounty, Kenya, and the occurrence of AMR in Staphylococcus isolates among the study subjects. Nasal swabs from 176 pastoralists were cultured, and the bacteria isolates identified using standard phenotypic and biochemical bacteriological methods. Among the obtained 195 isolates, the most prevalent isolates were coagulase-negative Staphylococcus (CoNS) (44.9%), followed by Enterococci spp. (43.2%) while Staphylococcus aureus prevalence was 8%. Antimicrobial sensitivity of the Staphylococcus spp. isolates to 14 antibiotics representing six antibiotic groups was undertaken using the Kirby-Bauer disk diffusion method. Among the CoNS, the highest resistance was reported in amoxicillin (78.7%) and ceftazidime (76%), while the most resistance for S. aureus was reported in ceftazidime (100%), amoxicillin (71.4%), and streptomycin (71.4%). From an administered questionnaire looking at gender, animal contact frequency, history of hospital visitation and antibiotic usage, and habitual intake of raw milk, the study showed that male participants had a higher risk of carrying multiple drug resistant (MDR) bacteria than females ( p = 0.02 , OR = 1.3 ). Likewise, habitual intake of raw milk was significantly associated MDR acquisition ( p = 0.02 , OR = 1.82 ). This study reveals a high prevalence of AMR Staphylococcus isolates in the study area laying a foundation for further analysis of molecular characterization of the observed resistance as well as the development of interventions that can reduce the occurrence of AMR in the study area.

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Detection of Integrons and Staphylococcal Cassette Chromosome mec Types in Clinical Methicillin-resistant Coagulase Negative Staphylococci Strains

Cited by 3 publications

References 34 publications

Investigation of SCCmec types I–IV in clinical isolates of methicillin-resistant coagulase-negative staphylococci in Ahvaz, Southwest Iran

Investigation of SCCmec types I–IV in clinical isolates of methicillin-resistant coagulase-negative staphylococci in Ahvaz, Southwest Iran

Characterization and molecular evaluation of Staphylococcus aureus isolated from poultry and dairy cattle milk in Iraq

Prevalence and Potential Risk Factors for the Acquisition of Antibiotic‐Resistant Staphylococcus spp. Bacteria Among Pastoralist Farmers in Kajiado Central Subcounty, Kenya

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