Detection of Influenza Viruses Attached to an Optical Disk

Shima, Takayuki; Fujimaki, Makoto; Yoshida, Akihiro; Gopinath, Subash C.B.; Kuwahara, Masashi; Ohki, Yoshimichi; Awazu, Kunio

doi:10.4236/jbnb.2013.42020

Cited by 9 publications

(7 citation statements)

References 12 publications

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“…10) However, the mark edges can be clearly recognized, and this is acceptable for the present study. 8) On the right-hand side of Fig. 3, some intensity dips were observed for the all scanned data for (a) to (h).…”

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confidence: 88%

“…There are a number of research papers on bacteria detection by optical methods [3][4][5] and on the use of optical disk technology for the detection of various biological substances. [6][7][8] Arai et al prepared a biotinylated disk sample surface to bind streptavidin and detected the reflected light intensity change caused by the attachment of the streptavidin to the surface. 6) Tsujita et al recently used NeutrAvidinÔimmobilized magnetic beads to enhance its binding with biotin ligands formed on a disk sample surface by applying a magnetic field.…”

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confidence: 99%

“…Further details on sample preparation can be found in our previous paper. 8) It should be noted that ITO was used for optical purposes only, i.e., to control the reflectance of the disk sample via its thickness and to create marks where the reflectance is different from the surroundings. An optical disk drive tester (Pulstec Industrial DDU-1000) with a laser wavelength () of 405 nm and a numerical aperture (NA) of 0.65 was used for recording marks and retrieving E. coli present on the disk sample.…”

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confidence: 99%

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Detection and Two-Dimensional Imaging of Escherichia coli Attached to an Optical Disk

Shima

Gopinath

Fujimaki

et al. 2013

Jpn. J. Appl. Phys.

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Escherichia coli detection was conducted using an optical disk drive tester. A sample of E. coli was mixed in deionized water to obtain an optical density of 0.1 at a wavelength of 600 nm. The solution was dropped onto an oxide-coated disk, on which positioning marks had been made, and dried. Two-dimensional imaging of the E. coli dispersed on the surface was carried out using the marks, and the image obtained was similar to that obtained by an optical microscope. The results indicate that this system can be used to identify the size and number of E. coli.

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confidence: 88%

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confidence: 99%

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confidence: 99%

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Detection and Two-Dimensional Imaging of Escherichia coli Attached to an Optical Disk

Shima

Gopinath

Fujimaki

et al. 2013

Jpn. J. Appl. Phys.

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“…[8][9][10] We also successfully obtained a scanned image of E. coli dispersed on an optical disk, using a commercially available optical disk system, by detecting reflected light from the optical disk. [11][12][13][14] However, we found that it was difficult to distinguish E. coli from other contaminants in the imaging results. Therefore, in this study, we developed a new optical disk system that can detect the intensity of the light transmitted through the optical disk and distinguish E. coli from other contaminants by staining treatment.…”

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confidence: 80%

Selective detection ofEscherichia coliby imaging of the light intensity transmitted through an optical disk

Shiramizu

Kuroda

Ohki

et al. 2018

Appl. Phys. Express

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We have developed an optical disk system for imaging transmitted light from Escherichia coli dispersed on an optical disk. When E. coli was stained using Bismarck brown, the transmittance was found to decrease in images obtained at λ = 405 nm. The results indicate that transmittance imaging is suitable for finding the difference in light intensity between stained and unstained E. coli, whereas the reflectance images were scarcely changed by staining. Therefore, E. coli can be selectively discriminated from abiotic contaminants using transmittance imaging.

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“…In that case, aptamer helps to detect the pathogenic biomarkers at the earlier stage. Different aptamers were generated against Influenza viral types A and B [3,[7][8][9]. Lakshmipriya et al [3] have selected aptamers against influenza B virus and detected influenza with the waveguide-mode sensor and reached the limit of detection to 100 picomolar (pM), which is a much lower level than influenza antibody-mediated detection.…”

Section: Diagnosis Of Pathogens By Aptamermentioning

confidence: 99%