Detection of influenza A virus RNA in birds by optimized Real—Time PCR system

Problems and Methods of Diagnosis of Respiratory Viral Infections of Birds

“…Unfortunately, despite the progression of PCR analysis, today in Russia, foreign test systems are mainly used to identify avian influenza viruses and Newcastle disease. From domestic developments, only a multiplex PCR test system developed by the Vector State Research Institute of Physics and Technology [29] can be considered a truly effective method. Its advantages include the 100% accuracy of identifying individual strains of influenza viruses, as well as the virus of Newcastle disease, and the joint testing of standard virus strains from the collection of the Vector did not affect the accuracy of the analysis Ilinykh [29].…”

“…From domestic developments, only a multiplex PCR test system developed by the Vector State Research Institute of Physics and Technology [29] can be considered a truly effective method. Its advantages include the 100% accuracy of identifying individual strains of influenza viruses, as well as the virus of Newcastle disease, and the joint testing of standard virus strains from the collection of the Vector did not affect the accuracy of the analysis Ilinykh [29]. However, even though the development has successfully passed production testing in several laboratories in Siberia and Kazakhstan, it remains unclaimed.…”