Detection of individual human neutrophil ?-defensins (human neutrophil peptides 1, 2 and 3) in unfractionated gingival crevicular fluid?A MALDI-MS approach

Lundy, Fionnuala; Orr, David F.; Shaw, Christopher; Lamey, P J; Linden, Gerard J.

doi:10.1016/j.molimm.2004.09.008

Cited by 50 publications

(69 citation statements)

References 12 publications

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“…Accordingly, human neutrophil defensin levels were under detection limit in the periodontal disease samples, whereas the mean concentration of the healthy samples was 125.13 ± 129.04 ng/ml. These findings are consistent with the LC/MS E data, as well as previously published data (Lundy et al, 2005). The fact that neutrophil defensin, which has an antimicrobial properties was absent in the diseased samples supports the idea that the progression of periodontal disease could be linked to a decrease in the defensin barrier (Lundy et al, 2005).…”

Section: Identification and Quantification Of Gcf Proteinssupporting

confidence: 92%

Application of Label-Free Absolute Quantitative Proteomics in Human Gingival Crevicular Fluid by LC/MS^E (Gingival Exudatome)

et al. 2010

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Periodontal disease is perhaps the most common infectious disease in humans. Gingival crevicular fluid (GCF) is a local inflammatory exudate of the periodontal tissues. Its composition greatly varies between health and periodontal disease. GCF collection is rapid and noninvasive, but previous approaches aiming to analyze its composition have mainly involved single protein biomarkers. The aim of this study was to perform analysis of the GCF exudatome from healthy and periodontally diseased sites by LC/MS E , a label-free mass spectrometry method that enables simultaneous protein identification and absolute quantification in biological fluids. In total, 154 proteins of human, bacterial, and viral origin were identified in the 40 GCF samples obtained from the 10 subjects (five healthy and five generalized aggressive periodontitis). The proportion of bacterial, viral, and yeast protein was increased in disease, compared to health. The presence of host defense-related proteins, such as Cystatin-B and defensins, was confirmed to be present only in health. Among the newly identified GCF proteins were L-plastin detected only in disease (15.6 ( 12.1 fmol) and Annexin-1 detected in 5-fold higher levels in health. Nevertheless, pro-inflammatory cytokines or periodontal pathogen proteins were rarely detected. Conclusively, the LC/MS E technology may facilitate characterization of GCF proteome in periodontal health and disease, thus conferring prognostic and diagnostic value. Larger cohort studies are required to characterize the complete GCF proteome in health and disease.

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Section: Identification and Quantification Of Gcf Proteinssupporting

confidence: 92%

Application of Label-Free Absolute Quantitative Proteomics in Human Gingival Crevicular Fluid by LC/MS^E (Gingival Exudatome)

et al. 2010

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“…As a large number of neutrophils continuously enter the oral cavity through the junctional epithelium surrounding the teeth, it is possible that the HNPs are mainly derived from these neutrophils. In fact, the presence of HNP1-4 in gingival crevicular fluid has been reported [27][28][29][30]. The results of an HNP1-3 ELISA assay revealed that the concentration of HNPs in gingival crevicular fluid was higher than that in whole saliva (Fig.…”

Section: Detection and Expression Of Defensins In Saliva And Salimentioning

confidence: 85%

Salivary Defensins and Their Importance in Oral Health and Disease

Abiko¹,

Saitoh

2007

CPD

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Saliva contributes significantly to the protective barrier of oral epithelium through its mechanical rinsing action and the unique peptides it contains. Saliva contains several types of antimicrobial peptides, including defensins, which may have an important role in innate host defense. Many types of human defensins have been discovered and characterized in the last decade. This review summarizes the recent literature on salivary defensins and discusses their importance in oral health and disease. Salivary defensins are possibly derived from salivary ductal cells, oral epithelial cells and some blood cells. The antimicrobial activity of defensins may be affected by the components of saliva. Salivary defensin levels can be altered in oral diseases, and therefore may be a useful marker for risk assessment, salivary diagnosis and therapeutic strategies.

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“…Alpha-defensins (HNP1-4) are located in the azurophilic granules of neutrophils, whereas HNP5-6 are present in the intestinal Paneth’s cells (Doss et al 2010; Lundy et al 2005). In oral environment, α-defensins are present in the salivary glands and saliva as well as in GCF (Brogden et al 2003).…”

Section: Introductionmentioning

confidence: 99%

“…Until now, very few articles have been published on the presence of GCF HNP1-3 in healthy subjects and in patients with chronic periodontitis (Lundy et al 2005; Puklo et al 2008; Türkoğlu et al 2010). Besides, to our knowledge, human neutrophil peptides have never been investigated after periodontal therapy.…”

Section: Introductionmentioning

confidence: 99%

The Effect of Nonsurgical Periodontal Therapy on HNP1-3 Level in Gingival Crevicular Fluid of Chronic Periodontitis Patients

Dolińska

Skurska

Pietruska

et al. 2017

Arch. Immunol. Ther. Exp.

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The rich bacterial flora of oral cavity is controlled by innate immune response, including antibacterial peptides and among them human neutrophil peptides 1–3 (HNP1-3). The knowledge of the involvement of HNPs in innate and acquired immunity of the periodontium is fragmentary. The aim of the study was to assess alterations in HNP1-3 levels in the gingival crevicular fluid (GCF) of chronic periodontitis patients before and after nonsurgical periodontal therapy. Nineteen patients with chronic periodontitis were qualified to the study. After periodontal examination, one site with pocket depth (PD) ≥4 mm was selected. All the patients received periodontal treatment involving scaling and root planing with additional systemic antibiotic therapy (Amoxicillin 375 mg three times daily and Metronidazole 250 mg three times daily for 7 days). Prior to therapy, 3 and 6 months after it, clinical periodontal parameters were measured and GCF was collected from previously chosen site. The level of HNP1-3 in GCF was determined by means of a commercially available enzyme-linked immunoassay kit. The periodontal therapy caused a statistically significant (p < 0.001) decrease in all the assessed clinical parameters at the sites of sample collection except for bleeding on probing. The level of HNP1-3 per measure point showed a statistically significant increase (baseline—3 months: p = 0.05, baseline—6 months: p = 0.007). Within the limits of the study, it can be stated that nonsurgical periodontal therapy with additional systemic administration of Amoxicillin and Metronidazole increases the level of HNP1-3 in GCF.

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Detection of individual human neutrophil ?-defensins (human neutrophil peptides 1, 2 and 3) in unfractionated gingival crevicular fluid?A MALDI-MS approach

Cited by 50 publications

References 12 publications

Application of Label-Free Absolute Quantitative Proteomics in Human Gingival Crevicular Fluid by LC/MS^E (Gingival Exudatome)

Application of Label-Free Absolute Quantitative Proteomics in Human Gingival Crevicular Fluid by LC/MS^E (Gingival Exudatome)

Salivary Defensins and Their Importance in Oral Health and Disease

The Effect of Nonsurgical Periodontal Therapy on HNP1-3 Level in Gingival Crevicular Fluid of Chronic Periodontitis Patients

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Detection of individual human neutrophil ?-defensins (human neutrophil peptides 1, 2 and 3) in unfractionated gingival crevicular fluid?A MALDI-MS approach

Cited by 50 publications

References 12 publications

Application of Label-Free Absolute Quantitative Proteomics in Human Gingival Crevicular Fluid by LC/MSE (Gingival Exudatome)

Application of Label-Free Absolute Quantitative Proteomics in Human Gingival Crevicular Fluid by LC/MSE (Gingival Exudatome)

Salivary Defensins and Their Importance in Oral Health and Disease

The Effect of Nonsurgical Periodontal Therapy on HNP1-3 Level in Gingival Crevicular Fluid of Chronic Periodontitis Patients

Contact Info

Product

Resources

About

Application of Label-Free Absolute Quantitative Proteomics in Human Gingival Crevicular Fluid by LC/MS^E (Gingival Exudatome)

Application of Label-Free Absolute Quantitative Proteomics in Human Gingival Crevicular Fluid by LC/MS^E (Gingival Exudatome)