“…Recently, several types of SPE columns have been commonly used for extraction of aminoglycosides in bio-samples, such as a strong-cation exchanger [11,12], a weak-cation exchanger [13,14], an RP C18 [15,16], and a mixed model of an ion exchanger combined with RP [17]. Using SPE column, targeting analytes could be cleaned up, purified, and concentrated easily from a complicated matrix.…”