Detection of immunoglobulin M antibodies with antigen-tagged latex particles in an immunosorbent assay

Remington, Jack S.; Eimstad, W M; Araújo, Francisco Gerson

doi:10.1128/jcm.17.5.939-941.1983

Cited by 23 publications

(2 citation statements)

References 5 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…This test is simpler and easier to perform than the IgM-ELISA, but it requires large numbers of T. gondii tachyzoites. Thereafter, the IgM-ISAGA is modified by replacing T. gondii tacyzoites with latex beads coated with soluble antigens [ 80 ]. IgM-ISAGA can be used for the diagnosis of acute acquired and congenital T. gondii infection.…”

Section: Reviewmentioning

confidence: 99%

Diagnosis of toxoplasmosis and typing of Toxoplasma gondii

et al. 2015

View full text Add to dashboard Cite

Toxoplasmosis, caused by the obligate intracellular protozoan Toxoplasma gondii, is an important zoonosis with medical and veterinary importance worldwide. The disease is mainly contracted by ingesting undercooked or raw meat containing viable tissue cysts, or by ingesting food or water contaminated with oocysts. The diagnosis and genetic characterization of T. gondii infection is crucial for the surveillance, prevention and control of toxoplasmosis. Traditional approaches for the diagnosis of toxoplasmosis include etiological, immunological and imaging techniques. Diagnosis of toxoplasmosis has been improved by the emergence of molecular technologies to amplify parasite nucleic acids. Among these, polymerase chain reaction (PCR)-based molecular techniques have been useful for the genetic characterization of T. gondii. Serotyping methods based on polymorphic polypeptides have the potential to become the choice for typing T. gondii in humans and animals. In this review, we summarize conventional non-DNA-based diagnostic methods, and the DNA-based molecular techniques for the diagnosis and genetic characterization of T. gondii. These techniques have provided foundations for further development of more effective and accurate detection of T. gondii infection. These advances will contribute to an improved understanding of the epidemiology, prevention and control of toxoplasmosis.

show abstract

Section: Reviewmentioning

confidence: 99%

Diagnosis of toxoplasmosis and typing of Toxoplasma gondii

et al. 2015

View full text Add to dashboard Cite

show abstract

“…While traditional diagnostic methods are based on serological tests and bioassays, a variety of molecular methods have been recently used for diagnosis of toxoplasmosis ( 14 ). Some of the diagnostic tests for toxoplasmosis include microscopy ( 15 ), bioassays ( 16 ), dye test ( 17 ), modified agglutination test ( 18 ), latex agglutination test ( 19 ), indirect hemagglutination test ( 20 ), indirect fluorescent antibody test ( 21 ), enzyme-linked immunosorbent assay ( 22 ), immunosorbent agglutination assay ( 23 ), immunochromatographic test ( 24 ), piezoelectric immunoagglutination assay ( 25 ), Western blot ( 26 ), and avidity test ( 27 ). Pharmaceutical interventions against toxoplasmosis include either a combination of pyrimethamine and sulfadiazine with folic acid or a combination of pyrimethamine and macrolide antibiotics or lincosamide.…”

Section: Introductionmentioning

confidence: 99%

A Cross-Sectional Study on the Association Between Risk Factors of Toxoplasmosis and One Health Knowledge in Pakistan

et al. 2021

View full text Add to dashboard Cite

Toxoplasmosis is a zoonotic disease caused by Toxoplasma gondii, a protozoan that infects warm-blooded animals and humans. Approximately one third of the global population is infected by T. gondii. We conducted a cross-sectional study to assess the risk factors and One Health knowledge of toxoplasmosis in Rawalpindi and Islamabad, Pakistan. From July through December 2020, we collected data using questionnaires. The results showed that 60% of participants had heard or read about the disease, 23.3% of participants had no knowledge about the disease, and 16.8% participants were not sure about the disease. More than half of the participants (53.3%) reported that toxoplasmosis was caused by toxins, 5.3% reported that toxoplasmosis was an animal disease, 13.8% reported that toxoplasmosis was a human disease, 65.8% reported that it was both an animal and human disease, and 15.3% reported that it was neither an animal nor a human disease. Approximately 80.5% of participants reported that individuals acquired toxoplasmosis by changing cat litter. Our study findings revealed a low level of knowledge and awareness about toxoplasmosis among males. Therefore, there should be awareness programs to educate individuals about the risks of this deadly disease and to provide information on the major routes of transmission.

show abstract

Pneumocystis carinii and Parasitic Infections in the Immunocompromised Host

Fishman

2002

Clinical Approach to Infection in the Compromised Host

View full text Add to dashboard Cite

Detection of immunoglobulin M antibodies with antigen-tagged latex particles in an immunosorbent assay

Cited by 23 publications

References 5 publications

Diagnosis of toxoplasmosis and typing of Toxoplasma gondii

Diagnosis of toxoplasmosis and typing of Toxoplasma gondii

A Cross-Sectional Study on the Association Between Risk Factors of Toxoplasmosis and One Health Knowledge in Pakistan

Pneumocystis carinii and Parasitic Infections in the Immunocompromised Host

Contact Info

Product

Resources

About