Detection Of Immune Complexes

Agnello, Vincent; Gabriel, Alexandre; Tai, Mindy M.

doi:10.1111/1523-1747.ep12514497

Cited by 26 publications

(13 citation statements)

References 15 publications

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“…However, the high IgG levels in serum still present an interference problem which is circumvented by using a 1:10 dilution of test sera. Sensitivity of the assay is proportionately decreased by the dilution step, but in spite of this, on the basis of the limited comparative data currently available, the test with mRF is more sensitive than any assay thus far well characterized (14).…”

Section: Resultsmentioning

confidence: 99%

“…J.) (14) and further purified by chromatography in Sephadex G-200 column (Pharmacia Fine Chemicals, Inc.) and zone electrophoresis (25). Purity of preparation was ascertained by radical immunodiffusion against anti-IgG, anti-IgM, and anti-IgA (Tri-Partigen Behring Diagnostics, American Hoechst Corp., Somerville, N.…”

Section: Reagentsmentioning

confidence: 99%

“…The possible role of immune complexes in a variety of basic immune mechanisms is currently under intensive study (1)(2)(3)(4) and in addition immune complexes have been shown to have an important role in a wide variety of diseases (5)(6)(7)(8)(9)(10)(11)(12). 1 Various techniques have been described for detecting immune complexes (13,14) but none so far have achieved the sensitivity, quantitative capacity, and simplicity for wide application. A major approach to the detection of immune complexes has been the use of reagents which will react preferentially with complexed immunoglobulins but not monomeric immunoglobulins.…”

Section: Introductionmentioning

confidence: 99%

“…Two such reagents are the Clq component of complement (15,16) and monoclonal anti-IgG (monoclonal rheumatoid factor, mRF) (17).2 These two reagents have been used for the detection of immune complexes in a variety of technical procedures, including more recently radioimmunoassays (18)(19)(20)(21)(22)(23). Tests with either reagent have limitations (14); however, when radioimmunoassay methods utilizing both reagents are run in parallel, the system has the capability of detecting both complementfixing and noncomplement-fixing immune complexes over a wide range of molecular weights.…”

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Detection of immune complexes. The use of radioimmunoassays with Clq and monoclonal rheumatoid factor.

Gabriel¹,

Agnello²

1977

J. Clin. Invest.

139

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Section: Resultsmentioning

confidence: 99%

Section: Reagentsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Detection of immune complexes. The use of radioimmunoassays with Clq and monoclonal rheumatoid factor.

Gabriel¹,

Agnello²

1977

J. Clin. Invest.

139

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“…Since immune complex assays may have false positive and false negative results because of the nature of the techniques and the size of the particles measured, a true investigation for immune complexes should include a search for the antigen and the antibody responsible for the reaction (25).…”

Section: Discussionmentioning

confidence: 99%

Survey for immune complexes in disseminated gonococcal arthritis‐dermatitis syndrome

Ludivico

Myers

1979

Arthritis & Rheumatism

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Seventeen patients with characteristic disseminated gonococcal arthritis-dermatitis syndrome were studied for the presence of immune complexes, and circulating gonococcal antigen and antibody. The two immune complex techniques, monoclonal rheumatoid factor assay and cryoglobulin survey, did not reveal any consistent abnormalities. Complement levels (CH50, C'3, C'4) were not consistent with peripheral consumption except in 2 patients with coinciding systemic lupus erythematosus. Gonococcal antibody was detected in 47% of patients when they presented with the syndrome. However, gonococcal antigen was not found in either serum or synovial fluid. These results do not support the hypothesis that circulating immune complexes are involved in the pathogenesis of disseminated gonococcal arthritis-dermatitis syndrome.The pathogenesis of the inflammatory lesions in disseminated gonococcal syndrome has been poorly understood and controversial. Various theories have been proposed to explain the skin and joint manifestations. The current explanation for the skin lesions has been

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Urticarial vasculitis: report of a case and review of the literature

Gammon¹

1979

Archives of Dermatology

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A woman with cutaneous vasculitis had a severe bullous eruption that was suggestive of erythema multiforme. The patient also had a history of recurrent urticaria that continued intermittently for over a year of follow-up examination. Skin biopsy specimens of both urticarial and erythema and multiforme lesions showed leukocytoclastic vasculitis. An illness resembling systemic lupus erythematosus (SLE) is suggested by transient, low-titer, positive antinuclear antibody tests, persistent deposits of immunoglobulin and complement in normal skin, arthralgias, circulating immune complexes, and chronic hypocomplementemia. This case is similar to cases previously reported as "hypocomplementemic vasculitis," an "unusual SLE-related syndrome," and "urticaria with vasculitis."

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Detection Of Immune Complexes

Cited by 26 publications

References 15 publications

Detection of immune complexes. The use of radioimmunoassays with Clq and monoclonal rheumatoid factor.

Detection of immune complexes. The use of radioimmunoassays with Clq and monoclonal rheumatoid factor.

Survey for immune complexes in disseminated gonococcal arthritis‐dermatitis syndrome

Urticarial vasculitis: report of a case and review of the literature

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