Detection of Illegal Abortion-Induced Drugs Using Rapid and Simultaneous Method for the Determination of Abortion-Induced Compounds by LC–MS/MS

Lee, Ji Hyun; Park, Han Na; Kim, Nam Sook; Park, Hyung-Joon; Park, Seongsoo; Shin, Dong-Chun; Kang, Hoil

doi:10.1007/s10337-019-03758-1

Cited by 5 publications

(6 citation statements)

References 19 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…However, the low concentration of mifepristone in fetal plasma indicates the need for an application of sensitive methods of RU-486 determination in postmortem material. Another noteworthy fact is that pills purchased online may contain different dosages from their legal counterparts [ 9 ]. Interest in abortifacient drugs has increased among individuals deciding to carry out pregnancy termination without specialist care.…”

Section: Resultsmentioning

confidence: 99%

“…As the number of illegal abortions increases, counterfeit medication abortifacients are becoming more widely available on the black market. Considering that the concentration of active ingredients can vary dramatically between different fake abortion-inducing drugs, their illegal distribution can pose a significant public health problem [ 9 ]. Mifepristone (one of the active ingredients of abortion pills) is used to induce an abortion during an unwanted pregnancy, and is illegal in some countries.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Determination of Mifepristone (RU-486) and Its Metabolites in Maternal Blood Sample after Pharmacological Abortion

Szpot

Wachełko²,

Zawadzki

2022

Molecules

View full text Add to dashboard Cite

The aim of the study was the development and validation of the UHPLC-QqQ-MS/MS method for the determination of mifepristone in human blood as well as the identification and quantification of its metabolites after self-induced pharmacological abortion. The metabolic pathway in humans was proposed after examination of an authentic casework. The fast and simple preanalytical procedure was successfully applied (pH9, tert-butyl-methyl ether). The validation parameters of the method were as follows: limit of quantification: 0.5 ng/mL; coefficients of determination: >0.999 (R2), intra- and inter-day accuracy and precision values did not exceed ± 13.2%. The recovery and matrix effect were in the range of 96.3–114.7% and from −3.0 to 14.7%, respectively. Toxicological analysis of the mother’s blood (collected the day after the pregnancy termination) revealed the presence of five compounds: mifepristone (557.4 ng/mL), N-desmethyl-mifepristone (638.7 ng/mL), 22-OH-mifepristone (176.9 ng/mL), N,N-didesmethyl-mifepristone (144.5 ng/mL) and N-desmethyl-hydroxy-mifepristone (qualitatively). To our knowledge, the study presented in this paper is the first report on the concentrations of mifepristone and its metabolites in maternal blood samples after performing a self-induced abortion. The established UHPLC-QqQ-MS/MS method is suitable for forensic toxicological analysis as well as in terms of clinical toxicology in future investigations (examination of pharmacokinetics, bioavailability and metabolism of RU-486).

show abstract

Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Determination of Mifepristone (RU-486) and Its Metabolites in Maternal Blood Sample after Pharmacological Abortion

Szpot

Wachełko²,

Zawadzki

2022

Molecules

View full text Add to dashboard Cite

show abstract

“…the Limit of Quantitation for mifepristone and misoprostol were found to be 1.65 μg/ml and 2.1 μg/ml [17]. In 2019, the study for detection of illegal abortion using counterfeit medicine has been carried out using LC-MS/MS instrumentation [18]. In 2015, a case study was conducted in which mifepristone was detected in segments of hair at four different segments after the intake of medicine [19].…”

Section: Figure 1 Structure Of Misoprostolmentioning

confidence: 99%

Identification of drugs using FTIR in a case of illegal abortion: A forensic study

Baskar¹,

Gupta²,

Singh³

2022

GSC Biol. and Pharm. Sci.

View full text Add to dashboard Cite

Illegal or criminal abortion is defined as termination of pregnancy in the violation of law. In many cases, doctors perform abortion without the consent of parents or only mothers, which is an offence against mother and/or father. Abortifacient are being frequently used for the same purpose, misoprostol is one of the drug, whose role is to expel dead fetus and placenta by controlling uterine muscles. The aim of this study was to identify misoprostol, a pharmaceutical drug using ATR technique of FTIR by identifying peaks which lies in fingerprint region. Five major peaks, 3304, 2912, 1731, 1364 and 1016 cm-1, were obtained, though as time lapses, peaks get deteriorate.

show abstract

“…Even today, there is still a lack of sufficient data to determine the safety of abortion pills sourced online, therefore further investigations are needed. Only a few methods such as FTIR [14], spectrophotometry [15], HPLC with UV detection [16] and tandem mass spectrometry [17,18] were applied for pharmaceutical drugs analysis.…”

Section: Introductionmentioning

confidence: 99%

Determination of Prostaglandins (Carboprost, Cloprostenol, Dinoprost, Dinoprostone, Misoprostol, Sulprostone) by UHPLC-MS/MS in Toxicological Investigations

Szpot,

Wachełko,

Zawadzki

2023

Toxics

View full text Add to dashboard Cite

Prostaglandins have stimulative influence on the human uterus and therefore were introduced to medical treatment in reproductive healthcare as labor inductors or abortifacients. The UHPLC-ESI-QqQ-MS/MS method was developed for six prostaglandins: carboprost, cloprostenol, dinoprost (PGF2α), dinoprostone (PGE2), misoprostol and sulprostone (substances for pregnancy termination) in pharmaceutical samples and was applied for the toxicological examination of pills containing misoprostol (collected during gynecological examination). There were used two internal standards: misoprostol-d5 and PGF2α-d4. The quantification of analytes was performed in the MRM mode. The linearity of method was in the range from 0.1 to 10 µg/mL, with a coefficient of determination above 0.997 (R2) for each compound. The precision and accuracy values did not exceed ±5.0%. Analysis of the pills revealed the presence of two substances: misoprostol and diclofenac. Misoprostol and diclofenac dose per sample were as follows: 608.8 ng (sample 1), 708.4 ng (sample 2), 618.8 ng (sample 3) and 67.7 mg (sample 1), 65.3 mg (sample 2) 67.3 mg (sample 3), respectively. A simple, precise and reliable method can be applied for routine examinations in terms of clinical and forensic toxicology examinations as well as in quality control of drugs for pharmaceutical purposes (original drugs and counterfeit medications).

show abstract

Detection of Illegal Abortion-Induced Drugs Using Rapid and Simultaneous Method for the Determination of Abortion-Induced Compounds by LC–MS/MS

Cited by 5 publications

References 19 publications

Determination of Mifepristone (RU-486) and Its Metabolites in Maternal Blood Sample after Pharmacological Abortion

Determination of Mifepristone (RU-486) and Its Metabolites in Maternal Blood Sample after Pharmacological Abortion

Identification of drugs using FTIR in a case of illegal abortion: A forensic study

Determination of Prostaglandins (Carboprost, Cloprostenol, Dinoprost, Dinoprostone, Misoprostol, Sulprostone) by UHPLC-MS/MS in Toxicological Investigations

Contact Info

Product

Resources

About