Detection of IgM antibodies to measles virus by enzyme-immunoassay

Tuokko, Hanna; Salmi, A.

doi:10.1007/bf02123492

Cited by 23 publications

(25 citation statements)

References 32 publications

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“…31, 1986, Supported by the Alma and K. A. Snellman Foundation, Oulu, Finland, and the Emil Aaltonen Foundation, Tampere, Finland. Submitted for publication May 12, 1989; accepted Aug. 15,1989. Reprint requests: Heikki Rantala, MD, Department of Pediatrics, University of Oulu, SF-90220 Oulu 22, Finland.…”

Section: Methodsmentioning

confidence: 99%

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Viral infections and recurrences of febrile convulsions

Rantala¹,

Uhari²,

Tuokko³

1990

The Journal of Pediatrics

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To determine whether complicated febrile seizures occur more often in children with a proven viral infection, we performed viral examinations on 144 children with febrile convulsions, of whom 112 had simple and 32 had complicated seizures. A diagnosis of virus infection was verified in 46% of the former patients and 53% of the latter. Three adenoviruses, one parainfluenza virus type 2 and one type 3, one respiratory syncytial virus, one echovirus type 11, one herpes simplex virus type 2, and one influenza B virus were isolated from the cerebrospinal fluid. A simple febrile convulsion occurred in seven children with a positive cerebrospinal fluid viral isolation, and two had a complex febrile seizure. In a follow-up of 2 to 4 years (mean 3.3 years), 21 of the 107 children with simple seizures (19.6%) and 3 of the 32 children with complicated seizures (9.4%) had recurrent febrile seizures. The children with positive evidence for a viral infection, even with a virus isolated from the cerebrospinal fluid, had no more recurrences than those without any proven viral infection. We conclude that children with a proven viral infection have no worse prognosis than those without.

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Section: Methodsmentioning

confidence: 99%

“…A similar classification has been used previously. 15 Samples of CSF (143 children), stools (130 children), and nasopharyngeal aspirates (134 children) were collected 195 RSV. Respiratory syneytial virus.…”

Section: Csfmentioning

confidence: 99%

Viral infections and recurrences of febrile convulsions

Rantala¹,

Uhari²,

Tuokko³

1990

The Journal of Pediatrics

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“…Nonspecific results in the indirect t~l?e of IgM antibody EIAs, caused by either rheumatoid factor' (12,14,16,23,24,27) or antibodies directed against cellular structures (2,14,22,23) are frequently encountered. Vgith the direct EIA (3,17,18,19,21,26) principle these nonspeeifie bindings should be minimal.…”

Section: Discussionmentioning

confidence: 98%

“…The results for tile serum specimens arriving at the laboratory in the afternoon could be given the next morning. The indirect measles EIA which has been used in our diagnostic unit for many years is performed in a single day, but the specificity tests of the positive specimens by adsorbing (23,24) rheumatoid factor require a day or two more.…”

Section: Discussionmentioning

confidence: 99%

“…The test principle in most of the viral antibody enzyme immunoassays reported earlier has also been that of the indirect type (9,11,24,27,28,30), where the solid p h a s e is first c o a t e d with viral antigens and the virus specific antibodies a t t a c h e d to t h e m are then detected b y enz~nne labelled antibodies specific to h u m a n IgG, I g M or IgA. H o w e v e r , this t y p e of IgM a s s a y is susceptible to interference from the r h e u m a t o i d factor (12,16,27) giving nonspeeifie positive results, when IgM is detected and specific I g G is present.…”

Section: Introductionmentioning

confidence: 98%

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Assay of measles virus IgM and IgG class antibodies by use of peroxidase-labelled viral antigens

Salonen

Vainionpää

Halonen

1986

Archives of Virology

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Purified measles virions, nucleocapsid protein and crude lysate of measles infected cells were labelled with horseradish peroxidase, and used for the detection of IgM and IgG antibodies to measles virus by direct enzyme immunoassay. The assays consisted of three layers: anti-human IgM or IgG immunoglobulins on solid-phase, test serum specimen and enzyme labelled viral antigen. For the expression of the results, a standard curve was included in each test and the O.D. values were changed to arbitrary antibody units. Specificity and sensitivity of the assays were compared with indirect EIAs. The specificity studies included a collection of serum specimens containing either rheumatoid factor, antinuclear antibodies or IgM antibody specific for other viruses. The assays proved both reliable and simple to perform and sensitivity was slightly higher than that for indirect EIAs. However, specificity was dependent on the purity of the viral antigens. When crude infected cell lysate antigen was used, some nonspecific results were obtained, particularly with serum specimens containing antinuclear antibodies. When virion or nucleocapsid protein were used, no nonspecific reactions were obtained.

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Prospective controlled survey of viral infections in children with acute lymphoblastic leukemia during chemotherapy

Möttönen

Uhari

Lanning

et al. 1995

Cancer

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Background. Infections cause significant morbidity in children with acute lymphoblastic leukemia (ALL). The incidence of viral infections commonly occurring in children with ALL receiving chemotherapy was compared with viral infections in control children and the spread of infections in families was traced. Methods. Fifteen families of children with ALL receiving chemotherapy (62 members) and 26 matched control families (106 members) were monitored for a total of 36,197 and 36,583 days, respectively, from November/1987 to December/1989 for the occurrence of infections. Results. The children with ALL had more infections than their control counterparts (P < 0.01) with respiratory infections the most common in both groups. Viral etiology was verified for 47 episodes [5.2/1000 days at risk, 95% confidence interval (CI) 3.9‐7.0] in the children with ALL and 22 (2.4/1000 days at risk, 95% CI 1.5‐3.7) in the control subjects (P < 0.01 for the difference). Parain‐fluenza viruses, enteroviruses, and adenoviruses were the most common agents in both groups. The infections of the other members of the patients' families were similar to those of their controls. The children most commonly fell ill first, and although children with ALL had more infections, they did not appear to spread the infections to their family members. Conclusions. The children with ALL had more infections than their controls, but they managed to recover well from viral infections caused by common respiratory viruses. The children with ALL did not change the spread of infections among family members.

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Detection of IgM antibodies to measles virus by enzyme-immunoassay

Cited by 23 publications

References 32 publications

Viral infections and recurrences of febrile convulsions

Viral infections and recurrences of febrile convulsions

Assay of measles virus IgM and IgG class antibodies by use of peroxidase-labelled viral antigens

Prospective controlled survey of viral infections in children with acute lymphoblastic leukemia during chemotherapy

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