Detection of<i>Trichinella</i>infection in slaughter horses by artificial digestion, ELISA and PCR

Viveros, Nicolás Mena; Arriaga, C.; Banda, V.; Ortega-Pierres, M. Guadalupe; Yépez‐Mulia, Lilián

doi:10.1051/parasite/200108s2257

Cited by 8 publications

(7 citation statements)

References 8 publications

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“…(94). These studies show that PCR was more sensitive in detecting the presence of Trichinella in some horses with late infections that were not detected by serologic methods (95).…”

Section: Use Of Tsl‐1 Antigens In Trichinella Detection and Diagnosismentioning

confidence: 99%

“…Therefore, additional, serologic studies were performed in horses raised in different geographical areas in Mexico to investigate the potential risk of human trichinellosis from horsemeat in Mexico. ELISA tests using TSL‐1 antigens allowed the specific detection of T. spiralis ‐infected animals with a prevalence of 7%–17% in rural and federal abattoirs, respectively (94,95); however, the ELISA only detected 25% (even at 1/20 serum dilution) of naturally infected horses which were determined to be infected when 40 g of meat were artificially digested (94). The low sensitivity of serologic methods for detection of horse trichinellosis has also been reported by Pozio et al .…”

Section: Use Of Tsl‐1 Antigens In Trichinella Detection and Diagnosismentioning

confidence: 99%

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Contributions to the study of Trichinella spiralis TSL‐1 antigens in host immunity

Yépez‐Mulia

Hernández-Bello

Arizmendi-Puga

et al. 2007

Parasite Immunology

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The observation on different hosts infected with Trichinella spiralis that recognized similar muscle larvae (ML) antigens and the fact that different monoclonal antibodies (mAb) had a similar reactivity to ML components prompted a proposal to define a useful classification system for these antigens. For this purpose, an international workshop provided a platform for the classification of T. spiralis antigens. ML antigens were classified in eight groups -- Trichinella spiralis larvae groups, TSL-1 to TSL-8. TSL-1 antigens are highly immunogenic and a number of important studies have been performed to analyse the role of these antigens in the host-parasite interplay. In this context, we have focused on the analysis of the role of TSL-1 antigens in the induction of innate immune responses with particular emphasis on the activation of mast cells (MC) by an IgE-independent pathway. These studies provided evidence on the role of mediator release from TSL-1-activated MC in the development of Type 2 immune responses. The protective role of TSL-1 in T. spiralis-infected mice has been described. In addition, it has been demonstrated that the use of TSL-1 antigens allows for a more sensitive and specific diagnosis of human and animal trichinellosis.

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“…(94). These studies show that PCR was more sensitive in detecting the presence of Trichinella in some horses with late infections that were not detected by serologic methods (95).…”

Section: Use Of Tsl‐1 Antigens In Trichinella Detection and Diagnosismentioning

confidence: 99%

Section: Use Of Tsl‐1 Antigens In Trichinella Detection and Diagnosismentioning

confidence: 99%

Contributions to the study of Trichinella spiralis TSL‐1 antigens in host immunity

Yépez‐Mulia

Hernández-Bello

Arizmendi-Puga

et al. 2007

Parasite Immunology

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“…The negative control was nuclease free water; which is usually used in the amplification session to verify the efficacy of the PCR system (Viveros et al, 2001 (Viveros et al, 2001).…”

Section: Polymerase Chain Reaction (Pcr) Amplificationmentioning

confidence: 99%

“…Fifteen microlitres of PCR MasterMix (composition: dATP 400µM, dCTP 400µM, dGTP 400µM, MgCl2 3mM, Taq DNA polymerase 50µ/m), 4µl of water, 1µl of oligonucleotides (primer sequence). (Viveros et al, 2001;Godaratollah and Saberivand, 2009;EURLP, 2016).…”

Section: Polymerase Chain Reaction (Pcr) Amplificationmentioning

confidence: 99%

Serological and Molecular Evaluation of Migratory Trichinella Spiralis Larvae in Blood of Humans in Kaduna Metropolis, Nigeria

Ojodale

Inabo

Ella

et al. 2021

FJS

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Trichinellosis is an important food-borne zoonotic disease with public health implications and a worldwide distribution. In this study, Polymerase Chain Reaction (PCR) procedure using species specific ATP6 primers was used to detect the presence of migratory Trichinella spiralis larval mitochondrial ATP6 synthase F0 subunit (ATP6) gene, after detection of antibodies to Trichinella excretory-secretory (E/S) antigen using Enzyme-linked Immunosorbent Assay (ELISA), in blood of humans in Kaduna metropolis, Nigeria. The sera of 210 participants were tested for antibodies to Trichinella E/S antigen. Overall seroprevalence rate of 39% (82/210) was recorded using ELISA. Out of the 9 ELISA samples selected randomly, PCR detected migratory Trichinella spiralis larval ATP6 gene in 4 (44.4%) at the amplicon size of 250 base pairs using the whole blood of the participants. The 9 samples comprised 7 seropositive and 2 seronegative. The bands at lanes 1, 2, 3 and 4 were positive for ATP6 while lanes 5,6,7,8 and 9 were negative for ATP6. Lanes 4 and 5 were ELISA negative for anti-Trichinella antibodies. One in 5 of the 128 ELISA negative samples was positive for ATP6 representing a 25.6% prevalence rate by extrapolation. PCR using ATP6 gene as a genetic marker is valuable for the detection of T. spiralis migratory larvae in blood samples of humans and consequently the early diagnosis of trichinellosis in humans.

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“…Therefore, the possibility of infection when the meat of these animals, raw or undercooked, is ingested by humans and synanthropic hosts is increased. 8,9 In this way, the possibility of contracting this parasitic increases by having more hosts with infection potential, and opens new routes of infection for humans.…”

Section: Introductionmentioning

confidence: 99%

Proteomic analysis of the excretion-secretion products of four Trichinella spiralis isolates obtained from accidental hosts

Franco-Sandoval¹,

Jiménez-Cardoso²,

Quezada³

et al. 2023

BMHIME

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Background: Trichinella spiralis is an intestinal and tissue nematode-specific for mammalian skeletal muscle, causing a series of physiological alterations. T. spiralis excretory-secretion products (ESP) play an important role in the appearance and regulation of these alterations. However, the effect of these products on the infection and invasion of the parasite to the host is unknown. Methods: Differences and similarities between antigenic proteins and surface proteins of four incidental hosts isolates (dogs) of T. spiralis and the reference strain isolated from pigs (MSUS/MEX/91/CM) were assessed by electrophoresis, western blot, and mass spectrometry. Results: Five proteins exclusive to the incidental hosts were analyzed using gene ontology. The results showed that these proteins are part of the extracellular matrix of the parasite, present catalytic activity, and bind to host cells. The antigenic activity of the four strains showed the antigenic triplet characteristic of T. spiralis of 43, 45 and 47 kDa. Conclusions: Five proteins exclusive to dog isolates provided information to understand the mechanism of action of this parasite to penetrate the muscle and evade the immune response in the host.

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Detection ofTrichinellainfection in slaughter horses by artificial digestion, ELISA and PCR

Cited by 8 publications

References 8 publications

Contributions to the study of Trichinella spiralis TSL‐1 antigens in host immunity

Contributions to the study of Trichinella spiralis TSL‐1 antigens in host immunity

Serological and Molecular Evaluation of Migratory Trichinella Spiralis Larvae in Blood of Humans in Kaduna Metropolis, Nigeria

Proteomic analysis of the excretion-secretion products of four Trichinella spiralis isolates obtained from accidental hosts

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