Detection of<i>Toxoplasma gondii</i>DNA by PCR in blood samples collected from pregnant Saudi women from the Aseer region, Saudi Arabia

Dajem, Saad M. Bin; Almushait, Mona

doi:10.5144/0256-4947.2012.14.7.1200

Cited by 23 publications

(13 citation statements)

References 27 publications

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“…Direct detection of acute Toxoplasma infection in humans can be performed by polymerase chain reaction (PCR), which amplifies the DNA of T. gondii in amniotic fluid, eye fluid, tissues, or blood [100,101]. For the rapid and sensitive detection of Toxoplasma infection, real-time PCR assays and targets including B1gene which occurs in 35 copies in T. gondii genome have been reported [102][103][104]. A 529-bp (GenBank Accession No.…”

Section: Molecular Diagnosticsmentioning

confidence: 99%

Serological and molecular rapid diagnostic tests for Toxoplasma infection in humans and animals

Khan

Noordin

2019

Eur J Clin Microbiol Infect Dis

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Infection by Toxoplasma gondii is prevalent worldwide. The parasite can infect a broad spectrum of vertebrate hosts, but infection of fetuses and immunocompromised patients is of particular concern. Easy-to-perform, robust, and highly sensitive and specific methods to detect Toxoplasma infection are important for the treatment and management of patients. Rapid diagnostic methods that do not sacrifice the accuracy of the assay and give reproducible results in a short time are highly desirable. In this context, rapid diagnostic tests (RDTs), especially with point-of-care (POC) features, are promising diagnostic methods in clinical microbiology laboratories, especially in areas with minimal laboratory facilities. More advanced methods using microfluidics and sensor technology will be the future trend. In this review, we discuss serological and molecular-based rapid diagnostic tests for detecting Toxoplasma infection in humans as well as animals.

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Section: Molecular Diagnosticsmentioning

confidence: 99%

Serological and molecular rapid diagnostic tests for Toxoplasma infection in humans and animals

Khan

Noordin

2019

Eur J Clin Microbiol Infect Dis

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“…T. gondii, is diagnosed mainly by serological methods that are hindered by insufficient sensitivity. When it fails, it becomes necessary to rely on either direct detection of the parasite or DNA detection by PCR [117].…”

Section: Toxoplasma Infectionmentioning

confidence: 99%

Study of Serum Copper and Iron in Children with Chronic Liver Diseases

Ibrahim¹

2013

Anat Physiol

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Trace elements have a major role as both oxidants and antioxidants, promoting and protecting from tissue damage respectively. As the liver has a pivotal role in trace elements metabolism and consequenyly their bioavailability, we aimed to measure serum levels of essential trace elements in children with chronic liver diseases (CLDs) regardless the etiology and to study their correlation with liver function tests. Serum zinc (Zn), copper (Cu) and iron (Fe); together with other trace elements parameters; were measured in 50 children with CLDs using spectrophotometry and their levels were compared with those age and sex matched healthy children as controls. Serum Cu, Fe, ferritin and transferrin saturation (TS) were significantly higher in the CLDs group than that in the control group; while serum Zn and total iron binding capacity (TIBC) were significantly lower in the CLDs than that in the control group. Serum Fe, Cu, TS and ferritin were positively correlated with biochemical parameters of liver damage; while serum Zn and TIBC were negatively correlated with biochemical parameters of liver damage. These results encourage inclusion of serum Zn, Cu and Fe as biomarkers for monitoring the severity of liver damage during routine assessment of children with CLDs. We recommended caution to avoid excess Fe and Cu intake in children with CLDs. Moreover, Zn supplementation could be encouraged in children with CLDs regardless its etiology.

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“…Two different specific primers were used for the first round of PCR (FP1: 5′- GGAACTGCATCCGTTCATGAG-3′; RP1: 5′-TCTTTAAAGCGTTCGTGGTC-3′) and for the second round (FP2: 5′-TGCATAGGTTGCAGTCACTG-3′; RP2: 5′-GGCGACCAATCTGCGAATACACC-3′) to amplify fragments of B1 genes to detect T. gondii [ 17 ]. The PCR reaction mixture (20 μl) was 6 μl sterile distilled water, 2 μl (100 ng/1 μl) extracted nDNA/first PCR product (for the first and second rounds, respectively), 1 μl forward primer (20 pmole), 1 μl reverse primer (20 pmole) and then 10 μl 2x master mix (Promega, USA) in 0.2 ml PCR eppendorf.…”

Section: Methodsmentioning

confidence: 99%

Maternal toxoplasmosis and the risk of childhood autism: serological and molecular small-scale studies

2021

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Background Toxoplasmosis resulting from infection with the Toxoplasma parasite has become an endemic disease worldwide. Recently, a few studies have reported a high prevalence of Toxoplasmosis infections among Saudi Arabian women. This disease could become life threatening for pregnant women and for immunodeficient people. There is evidence that infections during pregnancy, especially in the early stages, are associated with neurodevelopmental disorders. Autism disorder represents one of the most common neurodevelopmental disorders worldwide; it is associated with delayed language development, weak communication interaction, and repetitive behavior. The relationship between prenatal toxoplasmosis and autism in childhood remains unclear. The present study aims to report a link between maternal toxoplasmosis and autistic offspring among Saudi Arabian women. Method Blood samples (36 maternal, 36 from their non-autistic children, and 36 from their autistic children) were collected for serological and molecular evaluation. Results A toxoplasmosis infection was reported for 33.34% of participants using an ELISA assay (5.56% IgG+/IgM+, 11.11% IgG−/IgM+, and 16.67% IgG+/IgM-); however, a nested PCR assay targeting B1 toxoplasmosis specific genes recorded positive tests for 80.56% of the samples. In addition, the present study detected several points of mutation of mtDNA including NADH dehydrogenase (ND1, ND4) and Cyt B genes and the nDNA pyruvate kinase (PK) gene for autistic children infected with toxoplasmosis. Conclusion Considering previous assumptions, we suggest that a maternal toxoplasmosis infection could have a role in the development of childhood autism linked to mtDNA and nDNA impairment.

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Detection ofToxoplasma gondiiDNA by PCR in blood samples collected from pregnant Saudi women from the Aseer region, Saudi Arabia

Cited by 23 publications

References 27 publications

Serological and molecular rapid diagnostic tests for Toxoplasma infection in humans and animals

Serological and molecular rapid diagnostic tests for Toxoplasma infection in humans and animals

Study of Serum Copper and Iron in Children with Chronic Liver Diseases

Maternal toxoplasmosis and the risk of childhood autism: serological and molecular small-scale studies

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