Detection of <i>SYT</i> and <i>EWS</i> Gene Rearrangements by Dual-Color Break-Apart CISH in Liquid-Based Cytology Samples of Synovial Sarcoma and Ewing Sarcoma/Primitive Neuroectodermal Tumor

Kumagai, Arisa; Motoi, Toru; Tsuji, Kaori; Imamura, Tetsuo; Fukusato, Toshio

doi:10.1309/ajcptlsm15xkpddu

Cited by 14 publications

(8 citation statements)

References 28 publications

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“…CD99 also can be expressed in 35% of the cases, diffusely staining the cytoplasm. Cytogenetically, DSRCT is characterized by a reciprocal translocation, t(11;22)(p13;q12) 9, 60, 61, 64…”

Section: Introductionmentioning

confidence: 99%

Role of ancillary studies in fine‐needle aspiration from selected tumors

Schmitt

Barroca

2011

Cancer Cytopathology

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The routine use of ancillary studies is reshaping the practice of cytopathology. Currently, most cytopathologists recognize the importance of immunocytochemistry and molecular techniques as adjuncts to morphology to achieve a precise diagnosis. Cytopathologists also are expected to include specific prognostic and predictive information in their reports. The objective of this review was to address the use of immunocytochemistry and molecular techniques to refine the preoperative diagnosis and classification of lung cancer, thyroid cancer, kidney cancer, gastrointestinal cancer, and soft tissue tumors. Fine-needle aspiration also offers a suitable alternative to biopsy in a variety of clinical settings, in particular, when it may be useful to obtain material to study prognostic and predictive markers. This is particularly relevant to obtain material from metastatic sites. The study of KRAS in colon cancer, CKIT in gastrointestinal stromal tumors, and epidermal growth factor receptor mutational status in lung cancer also are addressed particularly in this report.

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“…CD99 also can be expressed in 35% of the cases, diffusely staining the cytoplasm. Cytogenetically, DSRCT is characterized by a reciprocal translocation, t(11;22)(p13;q12) 9, 60, 61, 64…”

Section: Introductionmentioning

confidence: 99%

Role of ancillary studies in fine‐needle aspiration from selected tumors

Schmitt

Barroca

2011

Cancer Cytopathology

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“…Immunohistochemical analysis of cervical smears containing a large number of the atypical cells of LCNEC may allow definitive preoperative diagnosis of the tumor or early diagnosis. There is a need to establish an immunostaining system for cytoblock and Liquid based cytology (LBC) [7].…”

Section: Discussionmentioning

confidence: 99%

A Study of Cervical Smear Cytology in Large Cell Neuroendocrine Carcinoma

Kanayama¹,

Yoneda²,

Hirokawa³

2012

J Cytol Histol

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We examined the possibility of diagnosing Large Cell Neuroendocrine Carcinoma (LCNEC) by cervical smear cytology. The study findings suggest that if a large number of consistent cytology characteristic features specific to LCNEC are observed in the specimen, such as salt and pepper chromatin, molding arrangement of the nuclei, rosette formation and palisade-like arrangement at the periphery of cell clumps, cytological diagnosis is possible.

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“…Dual-color break-apart probes were designed and generated as previously reported [11,12]. The dc-CISH procedure was previously described [11].…”

Section: Dual-color Chromogenic In Situ Hybridizationmentioning

confidence: 99%

“…Signals were visualized by Dako DuoCISH using an automated immunostainer (Autostainer plus; DAKO). As for signal interpretation, the rearrangement of SS18 gene was judged as definitely positive when isolated red and blue signals and a paired signal were observed in individual nuclei of tumor cells [12].…”

Section: Dual-color Chromogenic In Situ Hybridizationmentioning

confidence: 99%