Detection of <i>Staphylococcus aureus</i> with reduced susceptibility to vancomycin in a South African teaching hospital

Oosthuizen, Deon; Walsh, TR; Weldhagen, GP

doi:10.1080/10158782.2005.11441249

Cited by 2 publications

(2 citation statements)

References 1 publication

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“…Table 1, lists the randomly selected sample types and the number of isolates. Table 2, lists the vancomycin zone diameter 8 and MIC means and ranges. Table 3, lists the distribution of Staphylococci in the present study.…”

Section: Resultsmentioning

confidence: 99%

Utility of Kirby Bauer Disc Diffusion Method for Vancomycin Susceptibility Testing of Staphylococcal Isolates: Practicability, Acceptability, and Quality Aspects.

M¹,

Vidyasagar²,

Ravikumar³

2012

jemds

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Testing MIC of Vancomycin for all staphylococcal isolates is mandatory according to the current CLSI guidelines and this will considerably increase the cost of culture and sensitivity testing. This study is an attempt to re-consider the utility of the conventional disc diffusion method for cost-effective testing in resource poor settings. MATERIALS AND METHODS: Thirty coagulase positive and twenty eight coagulase negative staphylococci from various clinical samples have been randomly tested for minimum inhibitory concentration along with the Kirby Bauer disc diffusion method for Vancomycin over a period of five months. RESULTS: Susceptibility results of all the 58 isolates tested have been identical by both disc diffusion and HiComb MIC methods. Out of 58 isolates, 57 (98.26%) staphylococci have been sensitive to vancomycin by Kirby Bauer disc diffusion method as well as by the MIC testing method. The MICs of the susceptible strains have been <2 µg/mL. One isolate, a coagulase negative staphylococcus, has been tested to be resistant to vancomycin by both the methods with an MIC of 32 µg/mL SUMMARY: In our study of staphylococcal isolates from various clinical samples, there is overt significant concordance between disc diffusion and MIC testing methods in the routine susceptibility testing of vancomycin. CONCLUSION: Kirby Bauer disc diffusion method may still be of utility for routine testing of vancomycin susceptibility except for few cases especially in resource poor settings.

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Section: Resultsmentioning

confidence: 99%

Utility of Kirby Bauer Disc Diffusion Method for Vancomycin Susceptibility Testing of Staphylococcal Isolates: Practicability, Acceptability, and Quality Aspects.

M¹,

Vidyasagar²,

Ravikumar³

2012

jemds

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“…E-test vancomycin strips from BioMerieux, Marcy l'Etoile, France were used. As previously reported [15,16,17,18,19], the bacteria were grown for 18 hours at 37°C in Mueller Hinton broth. After diluting equal to McFarland no.0.5 tube, inoculums were applied using sterile swabs to Mueller Hinton agar.…”

Section: Mic Determination Of Vancomycin Intermediate Staphylococcus ...mentioning

confidence: 99%

Evaluation of Rosmarinus officinalis Leaves Essential Oils Activity Against Vancomycin Intermediate Staphylococcus aureus (VISA) Isolated from Baghdad Hospital Patients

Al-Hayali¹,

Marjani

Maleki

2023

Iraqi Journal of Science

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Rosemary is a well-known aromatic and medicinal plant used to treat various ailments. This study evaluated Rosmarinus officinalis essential oil for its phytochemical and antibacterial properties. The essential oil was analysed by using a gas chromatography-mass Spectrometry (GC-MS) that revealed the common chemicals containing verbenone 36.20% and 1,8-cineol (Eucalyptol) 12.14%. Extracted essential oils were tested for antibacterial activity against vancomycin intermediate Staphylococcus aureus (VISA), a strain of bacteria obtained locally from bacteremia patients. Three isolates were found to be VISA positive using the E-test (strips) and the population analysis profile method (PAP). VISA showed lower resistance to the essential oils (MIC: Minimum inhibitory concentration, MIC = 1.5 mg/ml). Overall, the genetic influence of VISA was evaluated by looking at the hld gene expression after being isolated from blood samples and RNA extraction for qRT-PCR to determine hld gene expression. R. officinalis gene expression was analysed and calculated by using real-time PCR of RNA which revealed that it had decreased.

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Detection of Staphylococcus aureus with reduced susceptibility to vancomycin in a South African teaching hospital

Cited by 2 publications

References 1 publication

Utility of Kirby Bauer Disc Diffusion Method for Vancomycin Susceptibility Testing of Staphylococcal Isolates: Practicability, Acceptability, and Quality Aspects.

Utility of Kirby Bauer Disc Diffusion Method for Vancomycin Susceptibility Testing of Staphylococcal Isolates: Practicability, Acceptability, and Quality Aspects.

Evaluation of Rosmarinus officinalis Leaves Essential Oils Activity Against Vancomycin Intermediate Staphylococcus aureus (VISA) Isolated from Baghdad Hospital Patients

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