Detection of<i>Ralstonia solanacearum</i>in ginger rhizomes by real-time PCR

Thammakijjawat, P.; Thaveechai, N.; Kositratana, W.; Chunwongse, Julapark; Frederick, Reid D.; Schaad, N. W.

doi:10.1080/07060660609507312

Cited by 15 publications

(12 citation statements)

References 21 publications

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“…Severe outbreaks of bacterial wilt of Zingiberaceae crops have been reported in several Asian countries (e.g., Indonesia, Thailand, Malaysia, India, the Philippines) 7,12,21,25,34 . Some of these outbreaks were caused by the use of infected rhizomes as planting material.…”

Section: Discussionmentioning

confidence: 99%

“…Newly introduced (or imported) seed rhizomes should be subject to a quarantine check, and the host cultivation history of the rhizomeproducing area (i.e., whether bacterial wilt disease has previously been recorded) must be investigated. Recently, immunodiagnostic and DNA-based detection assays for screening ginger rhizomes for specific pathogens have been developed 1,34 . Once a pathogen has been established and spreads in a cultivation area, a range of control methods (e.g., restricted seed rhizome management, soil fumigation, crop rotation with non-hosts, fallow) should be considered.…”

Section: Discussionmentioning

confidence: 99%

“…Bacterial wilt of Zingiberaceae plants has occurred in several countries or regions (e.g., Hawaii, Mauritius, some Asian countries, Aus tralia) 11,13,15,21,25,27,33,36,37,39,46,48 . The situation is made worse by the use of infected rhizomes as planting material 12,21,25,34,38 .…”

Section: Introductionmentioning

confidence: 99%

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Genetic Diversity of Zingiberaceae Plant Isolates of Ralstonia solanacearum in the Asia-Pacific Region

Waki

Horita

Kurose

et al. 2013

JARQ

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The genetic diversity of Ralstonia solanacearum strains isolated from Zingiberaceae plants in the Asia-Pacific region was assessed by examining their biochemical properties, discriminating the phylogeny by polymerase chain reaction (PCR), and analyzing the egl and mutS gene sequences. These data were compared with those of reference strains covering the known diversity within the R. solanacearum species complex. Fifty-two of the Zingiberaceae plant isolates belong to either biovar 3 or biovar 4. Multiplex PCR analyses indicated that these strains belong to phylotype I. Phylogenetic analyses revealed that the investigated strains could be further divided into five or more groups and three major groups, based on the egl and mutS gene sequences, respectively. These groups were closely correlated with the host species and/or geographical origin. Our findings suggest that R. solanacearum strains affecting Zingiberaceae plants have multiple origins from within the Asia-Pacific region, and may have been disseminated with seed rhizomes.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

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Genetic Diversity of Zingiberaceae Plant Isolates of Ralstonia solanacearum in the Asia-Pacific Region

Waki

Horita

Kurose

et al. 2013

JARQ

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“…Concerning the detection of subspecific lineages of Rs, some studies targeted the detection of specific races/biovars. Subtractive hybridization has led to the proposal of primers for specific detection of strains belonging to phylotype I (race 5/biovar 5) (Pan et al, 2013), and an amplified fragment length polymorphism-specific band was used to design a real-time PCR TaqMan assay specific for Rs race 1, biovars 3 and 4 (Thammakijjawat et al, 2006). Due to its importance as a widespread phytopathogen, several assays were designed to target specifically race 3/biovar 2 strains directly; namely a real-time BIO-PCR (Ozakman & Schaad, 2003), with primers based on the specific region described by Fegan et al (1998) and a loop-mediated isothermal amplification assay developed using bioinformatics to compare Rs fully-sequenced genomes (Kubota et al, 2008).…”

Section: Introductionmentioning

confidence: 99%

A quantitative hybridization approach using 17 DNA markers for identification and clustering analysis of Ralstonia solanacearum

et al. 2015

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Ralstonia solanacearum (Rs) is a quarantine phytopathogenic bacterium accountable for heavy economic losses worldwide. Monitoring and eradication programmes required for this pathogen are dependent on the availability of time-and cost-efficient detection and typing methods. However, members of the Rs species complex are characterized by a high phenotypic and genetic diversity, which requires improved diagnostics methods. The currently available full genome sequences of several Rs strains allow for the selection of novel specific DNA markers using comparative genomics tools. In this work, 17 novel markers were selected based on Rs-specific protein domains and thoroughly validated for specificity and stability, both in silico and using 'wet lab' assays. Polymerase chain reaction-and hybridization-based validation assays revealed that the DNA regions selected as markers were unevenly distributed amongst the tested strains, with nine markers present throughout the species complex. The distribution of the remaining eight markers was highly variable between the different analysed strains and enabled the attainment of strain-specific dot blot hybridization patterns, particularly informative for typing. The average probability value of each strain being positive for each of the 17 markers was calculated by an algorithm and used to obtain a dendrogram representing hierarchical clustering analysis of Rs, according to the similarity of their hybridization patterns. This method should prove to be a robust and straightforward procedure for genotyping members of the Rs species complex. Furthermore, this quantitative hybridization approach will allow the construction of informative databases to determine new Rs genotypes and infer epidemiological patterns.

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“…Kumar and Sarma (2004) detected Isolates of R. solanacearum of ginger with NCM-ELISA and biovars on the basis of membrane protein pattern on SDS-PAGE and biovar specific protein from R. solanacearum could be isolated. R. solanacearum was detected by PCR from rhizomes and soil (Kumar and Anandaraj, 2006;Kumar and Abraham, 2008) and using real time PCR from rhizomes (Thammakijjawat et al, 2006). Disease cycle and epidemiology: R. solanacearum spreads by infested soil adhering to hands, boots, tools, vehicle tires, and field equipment; in water from irrigation or rainfall; and by infected ginger rhizomes (Janse, 1996).…”

Section: Symptomsmentioning

confidence: 99%

Diseases infecting ginger (Zingiber officinale Roscoe): A review

Gupta

Kaushal

2017

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Ginger (Zingiber officinale Roscoe) is an important spice crop in India, which is also one of the leading producer and exporter of ginger in the world. During cultivation, the crop is severely infected by various diseases of them soft rot, yellows, Phyllosticta leaf spot, storage rot, bacterial wilt, mosaic, chlorotic fleck are important. These diseases reduce the potential yields drastically. The geographical distribution, losses, symptoms, causal organism, disease cycle, epidemiology and host resistance, cultural, biological, chemical and integrated management of above mentioned diseses have been discussed in the present paper.

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Detection ofRalstonia solanacearumin ginger rhizomes by real-time PCR

Cited by 15 publications

References 21 publications

Genetic Diversity of Zingiberaceae Plant Isolates of Ralstonia solanacearum in the Asia-Pacific Region

Genetic Diversity of Zingiberaceae Plant Isolates of Ralstonia solanacearum in the Asia-Pacific Region

A quantitative hybridization approach using 17 DNA markers for identification and clustering analysis of Ralstonia solanacearum

Diseases infecting ginger (Zingiber officinale Roscoe): A review

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