Detection of<i>Pseudomonas aeruginosa</i>quorum sensing molecules at an electrified liquid|liquid micro-interface through facilitated proton transfer

Burgoyne, Edward D.; Molina-Osorio, Andrés F.; Moshrefi, Reza; Shanahan, Rachel; McGlacken, Gerard P.; Stockmann, T. Jane; Scanlon, Micheál D.

doi:10.1039/d0an01245a

Cited by 14 publications

(3 citation statements)

References 82 publications

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“…½ � from equation 2 have been plotted in Figure 4 (markers) for the following ion-ligand combinations: Fe 2 + -phen (A), Fe 2 + -SAL (B), and H + -phen (C). Linear regression fittings (solid lines) and the standard deviation with respect to the linear regression analysis (dashed curves) using a 95 % confidence interval as described in Skoog et al [31] and recently [32] are also plotted in Figure 4. A definitive trend for the Fe 2 + complexation with N2N and TAC could not be decerned through CV and were instead analyzed using differential pulse voltammetry (DPV) as described below.…”

Section: Resultsmentioning

confidence: 99%

Electrochemical Characterization of Fe(II) Complexation Reactions at an Electrified Micro Liquid‐Liquid Interface

2021

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Dedicated to Prof. Marcin Opallo on the occasion of his 65th birthday!Phytoplankton growth in the open ocean is critical to carbon fixation and often limited by low iron concentrations. Owing to its low solubility, phytoplankton employ ligands for iron uptake; therefore, ligand binding characterization is important. Herein, a micro immiscible liquid/liquid interface was employed with four ligands through an electrochemically induced facilitated ion-transfer process. Ligands 1,10-phenathroline (phen), 1nitroso-2-naphthol (N2N), 2-(2-thiazolylazo)-p-cresol (TAC), and salicylaldoxime (SAL), where tested using established facilitated ion-transfer thermodynamics combined with differential pulse voltammetry. Three metal ion/ligand binding stoichiometries were observed for Fe 2 + :phen, whereas only one was observed with Fe 2 + with SAL, N2N, and TAC. Overall binding constants were calculated such that binding strengths can be ranked highest to lowest as phen > N2N > TAC > SAL. Additionally, the formal ion transfer for Fe 2 + (0.66 V) for an aqueous/1,2-dichloroethane interface was also determined.

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Section: Resultsmentioning

confidence: 99%

Electrochemical Characterization of Fe(II) Complexation Reactions at an Electrified Micro Liquid‐Liquid Interface

2021

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“…This fact improved the measurement and allowed the direct quantification of AQs and PYO in PAO1 bacterial cultures after 10 h growth without the need of extraction steps with organic solvents, yet obtaining worse limits of detection and dynamic ranges in bacterial culture broth. The last and most recent electrochemical detection of AQs was developed by Burgoyne and co-workers [131,132]. The technique is based on the detection of HHQ and PQS at the liquid-liquid interface between two immiscible electrolytic solutions.…”

Section: Electrochemical Sensorsmentioning

confidence: 99%

Biological and clinical significance of quorum sensing alkylquinolones: current analytical and bioanalytical methods for their quantification

Montagut

Marco

2021

Anal Bioanal Chem

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Quorum sensing (QS) is a sophisticated bacterial communication system which plays a key role in the virulence and biofilm formation of many pathogens. The Pseudomonas aeruginosa QS network consists of four sets of connected systems (las, rlh, pqs and iqs) hierarchically organized. The pqs system involves characteristic autoinducers (AI), most of them sharing an alkylquinolone (AQ) structure, and is able to carry out several relevant biological functions besides its main signalling activity. Their role in bacterial physiology and pathogenicity has been widely studied. Indeed, the presence of these metabolites in several body fluids and infected tissues has pointed to their potential value as biomarkers of infection. In this review, we summarize the most recent findings about the biological implications and the clinical significance of the main P. aeruginosa AQs. These findings have encouraged the development of analytical and bioanalytical techniques addressed to assess the role of these metabolites in bacterial growth and survival, during pathogenesis or as biomarkers of infections. The availability of highly sensitive reliable analytical methods suitable for clinical analysis would allow getting knowledge about pathogenesis and disease prognosis or progression, supporting clinicians on the decision-making process for the management of these infections and guiding them on the application of more effective and appropriate treatments. The benefits from the implementation of the pointof-care (PoC)-type testing in infectious disease diagnostics, which are seen to improve patient outcomes by promoting earlier therapeutic interventions, are also discussed.

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“…For the second step, the capillary containing the organic phase is transferred to a mildly acidic (pH 3) AME-free solution for a voltammetric detection through proton transfer assisted by AME. The presence of neutral molecules dissolved in the organic phase can indeed facilitate the transfer of proton across the interface by lowering the Gibbs free energy of transfer [37][38][39][40][41][42][43][44][45].…”

Section: Introductionmentioning

confidence: 99%

Ametryn detection by proton assisted transfer at a single micro-interface between two immiscible electrolyte solutions

Despas

Liu

et al. 2020

Journal of Electroanalytical Chemistry

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HAL is a multi-disciplinary open access archive for the deposit and dissemination of scientific research documents, whether they are published or not. The documents may come from teaching and research institutions in France or abroad, or from public or private research centers. L'archive ouverte pluridisciplinaire HAL, est destinée au dépôt et à la diffusion de documents scientifiques de niveau recherche, publiés ou non, émanant des établissements d'enseignement et de recherche français ou étrangers, des laboratoires publics ou privés.

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Detection ofPseudomonas aeruginosaquorum sensing molecules at an electrified liquid|liquid micro-interface through facilitated proton transfer

Abstract: Miniaturization of electrochemical detection of Pseudomonas aeruginosa quorum sensing molecules at a liquid/liquid interface through facilitated proton transfer reactions.

Cited by 14 publications

References 82 publications

Electrochemical Characterization of Fe(II) Complexation Reactions at an Electrified Micro Liquid‐Liquid Interface

Electrochemical Characterization of Fe(II) Complexation Reactions at an Electrified Micro Liquid‐Liquid Interface

Biological and clinical significance of quorum sensing alkylquinolones: current analytical and bioanalytical methods for their quantification

Ametryn detection by proton assisted transfer at a single micro-interface between two immiscible electrolyte solutions

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