Detection of <i>Pseudomonas aeruginosa</i> in the Skin by Immunomagnetic Isolation and Real-Time Quantitative PCR

Zhong, Daoqing; He, Wei-Qiang

doi:10.1166/jnn.2019.16595

Cited by 7 publications

(1 citation statement)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The immune-captured target cells were detected in beverages like milk and lemon juice by multiplex PCR and real-time PCR with a detection limit of 10 4 cfu•mL −1 and 10 3 cfu•mL −1 , respectively. Zhong et al [68] extracted DNA by magnetic particles to produce high-quality DNA for real-time quantitative PCR using an optimized set of primers. The method was highly sensitive, as it was capable of detecting as little as 100 cfu of P. aeruginosa.…”

Section: The Effects Of Nps In Real-time Pcrmentioning

confidence: 99%

Application of Nanomaterials to Enhance Polymerase Chain Reaction

et al. 2022

View full text Add to dashboard Cite

Polymerase Chain Reaction (PCR) is one of the most common technologies used to produce millions of copies of targeted nucleic acid in vitro and has become an indispensable technique in molecular biology. However, it suffers from low efficiency and specificity problems, false positive results, and so on. Although many conditions can be optimized to increase PCR yield, such as the magnesium ion concentration, the DNA polymerases, the number of cycles, and so on, they are not all-purpose and the optimization can be case dependent. Nano-sized materials offer a possible solution to improve both the quality and productivity of PCR. In the last two decades, nanoparticles (NPs) have attracted significant attention and gradually penetrated the field of life sciences because of their unique chemical and physical properties, such as their large surface area and small size effect, which have greatly promoted developments in life science and technology. Additionally, PCR technology assisted by NPs (NanoPCR) such as gold NPs (Au NPs), quantum dots (QDs), and carbon nanotubes (CNTs), etc., have been developed to significantly improve the specificity, efficiency, and sensitivity of PCR and to accelerate the PCR reaction process. This review discusses the roles of different types of NPs used to enhance PCR and summarizes their possible mechanisms.

show abstract

Section: The Effects Of Nps In Real-time Pcrmentioning

confidence: 99%