Detection of
            <i>Prochlorothrix</i>
            in Brackish Waters by Specific Amplification of
            <i>pcb</i>
            Genes

Geiß, Ulrike; Bergmann, Ingo; Blank, Miriam; Schumann, Rhena; Hagemann, Martin; Schoor, Arne

doi:10.1128/aem.69.10.6243-6249.2003

Cited by 13 publications

(13 citation statements)

References 37 publications

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“…Interestingly, although the filamentous Prochlorothrix is known to be a genus sensitive to high salinity, the uncommon species P. hollandica has been found in the Baltic Sea, in an environment with salinity up to 12 psu (BurgerWiersma et al, 1989;Geiss et al, 2003). In Sweden and in the Netherlands particularly, the most commonly reported cyanobacteria have been Microcystis aeruginosa, Aphanizomenon flosaquae, Oscillatoria spp., Planktothrix spp.…”

Section: Introductionmentioning

confidence: 99%

“…E-mail: vmvascon@ fc.up.pt Tan, 2007). The cyanobacteria of brackish waters (including estuaries), however, remain under-investigated, particularly in Europe, despite a number of significant contributions (Sivonen et al, 1989;Nehring, 1993;Lehtima¨ki et al, 1994Lehtima¨ki et al, , 1997Lehtima¨ki et al, , 2000Wille´n & Mattson, 1997;Sivonen & Jones, 1999;Sipia¨et al, 2001Sipia¨et al, , 2002Sipia¨et al, , 2006Sipia¨et al, , 2007Sipia¨et al, , 2008Vasconcelos & Cerqueira, 2001;Laamanen et al, 2002;Rocha et al, 2002;Geiss et al, 2003;Hobson & Fallowfield, 2003;Lehtonen et al, 2003;Stal et al, 2003;Sobrino et al, 2004;Herfindal et al, 2005;Kankaanpa¨a¨et al, 2005;Karjalainen et al, 2005Karjalainen et al, , 2007Karlsson et al, 2005;Pere´z & Carrilo, 2005;Surakka et al, 2005;Marino et al, 2006;Mazur-Marzec et al, 2007Paoli et al, 2007;Halinen et al, 2008;Ibelings & Havens, 2008;Jo´z´wiak et al, 2008;Lopes et al, 2010;Oftedal et al, 2010;). This lack of information for brackish-water cyanobacteria is especially significant in relation to the potential for production of new bioactive compounds.…”

Section: Introductionmentioning

confidence: 99%

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Planktonic and benthic cyanobacteria of European brackish waters: a perspective on estuaries and brackish seas

Lopes

Vasconcelos

2011

European Journal of Phycology

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Cyanobacteria are photosynthetic organisms found in aquatic and terrestrial biotopes and ecosystems throughout the world. Marine and freshwater cyanobacteria have been extensively studied due to the toxic hazards that some of them create and biotechnological interest. In contrast, the cyanobacteria of brackish waters have been less studied despite being able to form toxic blooms like their freshwater and marine counterparts. We review the occurrence, diversity and toxicity of cyanobacteria species in the brackish waters of Europe, mainly estuaries and brackish environments of the Atlantic Ocean and the Mediterranean and Baltic Seas. The dominant cyanobacteria belong mainly to the planktonic genera Nodularia, Aphanizomenon, Microcystis and Anabaena, but also the benthic forms of Anabaena and Phormidium. Most genera from brackish waters are reported to be hepatotoxin producers (microcystin and nodularin). However, anatoxin-a and other bioactive compounds (e.g. apoptogens) can also be found and are produced exclusively by benthic forms. Nodularin is the best-characterized brackish-water cyanotoxin, being primarily produced by N. spumigena. Data are presented on cyanotoxin production, accumulation, and potential food chain transfer, with a particular focus on nodularin, which induces multiple effects on food-chain dynamics. Microcystins and anatoxin-a are also considered. Potential risks to animals and humans from cyanotoxin exposure are discussed, although reports of animal poisoning by brackish-water toxic cyanobacteria are scarce and no cases of human intoxication are yet known.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Planktonic and benthic cyanobacteria of European brackish waters: a perspective on estuaries and brackish seas

Lopes

Vasconcelos

2011

European Journal of Phycology

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“…Prochlorothrix hollandica and pcbC gene detection: To detect the presence of Prochlorothrix hollandica, the DNA extracted from each environmental sample was submitted to a PCR amplification using the 16S-Pholl-fw/16S-Pholl-rev primer pair that specifically targets the 16S rRNA gene of P. hollandica (Geiss et al 2003). In addition, PCR detection of the gene pcbC of the operon encoding the chl a/b binding protein was carried out using primers pcbC-fw and pcbCrev as described by Geiss et al (2003) to detect chl bcontaining oxyphotobacteria.…”

Section: Methodsmentioning

confidence: 99%

“…In addition, PCR detection of the gene pcbC of the operon encoding the chl a/b binding protein was carried out using primers pcbC-fw and pcbCrev as described by Geiss et al (2003) to detect chl bcontaining oxyphotobacteria.…”

Section: Methodsmentioning

confidence: 99%

“…A positive signal was obtained for samples 2SU39, 2JE36, 2NI33, 2TL33 and 2SZ33. As P. hollandica possesses chl b, the environmental samples were submitted to PCR amplifications using the primer pair pcbC-fw/pcbC-rev that specifically targets the pcbC gene (Geiss et al 2003). Though pcbC is present in the P. hollandica strains, it is not known whether it is also present in the subcluster 'Prochlorothrix-like', because this group is only known from its 16S rRNA sequences.…”

Section: Cya359f-cya781rgc(a)mentioning

confidence: 99%

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Diversity of planktonic cyanobacteria and microcystin occurrence in Polish water bodies investigated using a polyphasic approach

Boutte¹,

Mankiewicz-Boczek²,

Komárková³

et al. 2008

Aquat. Microb. Ecol.

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Microscopic measurements of fresh biomass and 16S rRNA gene sequences from clone libraries and denaturing gradient gel electrophoresis (DGGE) were used to investigate cyanobacterial diversity in Polish water bodies in 2002. In addition, measurements of microcystin (MC) concentrations were made. Thirty water samples were taken from 11 water bodies; of these samples, 18 were obtained from the Sulejow Reservoir during regular monitoring from June to October. Intraand extracellular MC concentrations in Sulejow samples were measured by high performance liquid chromatography (HPLC). The extracellular MC concentration was assessed using a protein phosphatase inhibition assay (PPIA) in additional lakes. Additionally, physicochemical parameters were measured (total nitrogen [TN], total phosphorus [TP], TN:TP ratio, chlorophyll a concentration, temperature). In Sulejow, high intracellular MC concentrations corresponded to large cyanobacterial biovolumes and to low TN:TP ratios. In the other lakes, extracellular MCs were not linked to any measured parameters. The combination of the microscopic and molecular data showed that Aphanizomenon and Microcystis were the dominant genera during the summer period in the Sulejow Reservoir. At the genetic level, there was a succession of 2 different operational taxonomic units (OTUs) belonging to the lineage Anabaena/Aphanizomenon. In the other water bodies, the most frequent populations were Aphanizomenon, Anabaena, Microcystis and Planktothrix. Small populations of Romeria, Snowella, Woronichinia, Limnothrix and Pseudanabaena were observed, and an enigmatic cluster affiliated with Prochlorothrix was genetically retrieved. Anabaena and Microcystis were presumed to be the main genera responsible for the MC production.

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Darß-Zingst Boddens, Northern Rügener Boddens and Schlei

Schiewer¹

2008

Ecological Studies

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The Darß-Zingst Boddens (DZB) represents the typical bodden coasts of northern Germany, which stretch from the Fischland-Darß-Zingst peninsula via the islands of Hiddensee, Rügen and Usedom to the Polish island of Wolin. The "double coasts" show the typical features of level coasts at the seashore while, at the back, the indented boddens and lagoons are inner seawaters that are subject to a progressive phytogenic silting-up.The "jointed" structure of the DZB is also found in the Northern Rügener Bodden and at the mouth of the river Schlei ( Fig. 3.1), and these areas are therefore considered together here. Investigations into the DZB were sporadic at first (Gessner 1937;Hupfer 1959Hupfer , 1960Fukarek 1961;Schwarz 1961aSchwarz , 1961bSchwarz , 1964Brosin 1965aBrosin , 1965bHoppe and Pankow 1968;Hübel 1969). This changed fundamentally in the mid-1960s. The DZB has become the main area of investigation under the new focus on ecology and marine biology at Rostock University. Darß-Zingst Boddens Environmental CharacteristicsThe climate of the Baltic coast is determined by the "Ostseeklima". According to Kliewe (1951) and Hurtig (1957), a coastal area stretching 20-30 km landwards is basically involved. The "Ostseeklima" is identified by a transitional character with a relatively constant temperature, and by a delay in the start of the spring and summer seasons followed by mild autumns and relatively short winters. There is only little rain (405-790 mm per year), falling usually during autumn and winter. The mean annual air temperature is 9. 1°C (1995-2004). The number of frosty days is around 75-80 (Niedermeyer et al. 1987). The main wind direction is southwest/ west, with 80% of all winds reaching an average speed of 1.3-5 m s

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Detection of Prochlorothrix in Brackish Waters by Specific Amplification of pcb Genes

Cited by 13 publications

References 37 publications

Planktonic and benthic cyanobacteria of European brackish waters: a perspective on estuaries and brackish seas

Planktonic and benthic cyanobacteria of European brackish waters: a perspective on estuaries and brackish seas

Diversity of planktonic cyanobacteria and microcystin occurrence in Polish water bodies investigated using a polyphasic approach

Darß-Zingst Boddens, Northern Rügener Boddens and Schlei

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