Detection of <i>Porphyromonas gingivalis</i> DNA in Aortic Tissue by PCR

Stelzel, Michael; Conrads, Georg; Pankuweit, Sabine; Maisch, Bernhard; Vogt, Sebastian; Moosdorf, Rainer; Flores-de-Jacoby, L

doi:10.1902/jop.2002.73.8.868

Cited by 77 publications

(71 citation statements)

References 12 publications

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“…However, Hujoel et al (12) reported that their epidemiologic study did not find any evidence of an association between periodontal disease and heart disease. Nevertheless, members of our group and others have demonstrated that periodontal bacterial DNA can be detected in atherosclerotic lesions of aortic tissue (3,11,25,34).…”

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confidence: 90%

Correlation between Detection Rates of Periodontopathic Bacterial DNA in Carotid Coronary Stenotic Artery Plaque and in Dental Plaque Samples

et al. 2004

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Utilizing PCR, the 16S rRNA detection rates for Porphyromonas gingivalis, Actinobacillus actinomycetemcomitans, Bacteroides forsythus, Treponema denticola, and Campylobacter rectus in samples of stenotic coronary artery plaques were determined to be 21.6, 23.3, 5.9, 23.5, and 15.7%, respectively. The detection rates for P. gingivalis and C. rectus correlated with their presence in subgingival plaque.

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confidence: 90%

Correlation between Detection Rates of Periodontopathic Bacterial DNA in Carotid Coronary Stenotic Artery Plaque and in Dental Plaque Samples

et al. 2004

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“…Furthermore, in a study where bacterial DNA was identified in atherosclerotic plaques, 51.5% of the patients tested positive for Chlamydia in their atheromas (17). Several studies suggest an oral source for atherosclerotic plaque-associated bacteria (18)(19)(20)(21). However, to date, no single study has directly compared the microbial diversity of oral, gut, and atherosclerotic plaque microbiotas within individuals.…”

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confidence: 99%

Human oral, gut, and plaque microbiota in patients with atherosclerosis

Koren

Spor

Felin

et al. 2010

Proc. Natl. Acad. Sci. U.S.A.

976

756

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Periodontal disease has been associated with atherosclerosis, suggesting that bacteria from the oral cavity may contribute to the development of atherosclerosis and cardiovascular disease. Furthermore, the gut microbiota may affect obesity, which is associated with atherosclerosis. Using qPCR, we show that bacterial DNA was present in the atherosclerotic plaque and that the amount of DNA correlated with the amount of leukocytes in the atherosclerotic plaque. To investigate the microbial composition of atherosclerotic plaques and test the hypothesis that the oral or gut microbiota may contribute to atherosclerosis in humans, we used 454 pyrosequencing of 16S rRNA genes to survey the bacterial diversity of atherosclerotic plaque, oral, and gut samples of 15 patients with atherosclerosis, and oral and gut samples of healthy controls. We identified Chryseomonas in all atherosclerotic plaque samples, and Veillonella and Streptococcus in the majority. Interestingly, the combined abundances of Veillonella and Streptococcus in atherosclerotic plaques correlated with their abundance in the oral cavity. Moreover, several additional bacterial phylotypes were common to the atherosclerotic plaque and oral or gut samples within the same individual. Interestingly, several bacterial taxa in the oral cavity and the gut correlated with plasma cholesterol levels. Taken together, our findings suggest that bacteria from the oral cavity, and perhaps even the gut, may correlate with disease markers of atherosclerosis.

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“…These virulence factors include lipopolysaccharide, polysaccharide capsule, fimbriae, hemagglutinins, and several different proteinases (16,19,40). In addition, several studies have detected P. gingivalis in aortic tissue or arterial plaque samples (17,38,70), suggesting a relationship between this oral pathogen and cardiovascular disease.…”

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confidence: 99%

Comprehensive Transcriptome Analysis of the Periodontopathogenic Bacterium Porphyromonas gingivalis W83

Høvik

Yu²,

Olsen

et al. 2012

J Bacteriol

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High-density tiling microarray and RNA sequencing technologies were used to analyze the transcriptome of the periodontopathogenic bacterium Porphyromonas gingivalis. The compiled P. gingivalis transcriptome profiles were based on total RNA samples isolated from three different laboratory culturing conditions, and the strand-specific transcription profiles generated covered the entire genome, including both protein coding and noncoding regions. The transcription profiles revealed various operon structures, 5=-and 3=-end untranslated regions (UTRs), differential expression patterns, and many novel, not-yetannotated transcripts within intergenic and antisense regions. Further transcriptome analysis identified the majority of the genes as being expressed within operons and most 5= and 3= ends to be protruding UTRs, of which several 3= UTRs were extended to overlap genes carried on the opposite/antisense strand. Extensive antisense RNAs were detected opposite most insertion sequence (IS) elements. Pairwise comparative analyses were also performed among transcriptome profiles of the three culture conditions, and differentially expressed genes and metabolic pathways were identified. With the growing realization that noncoding RNAs play important biological functions, the discovery of novel RNAs and the comprehensive transcriptome profiles compiled in this study may provide a foundation to further understand the gene regulation and virulence mechanisms in P. gingivalis. The transcriptome profiles can be viewed at and downloaded from the Microbial Transcriptome Database website, http://bioinformatics.forsyth.org/mtd.

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Detection of Porphyromonas gingivalis DNA in Aortic Tissue by PCR

Abstract: These results might indicate a link between periodontopathogens entering the cardiovascular system and cardiovascular disease.

Cited by 77 publications

References 12 publications

Correlation between Detection Rates of Periodontopathic Bacterial DNA in Carotid Coronary Stenotic Artery Plaque and in Dental Plaque Samples

Correlation between Detection Rates of Periodontopathic Bacterial DNA in Carotid Coronary Stenotic Artery Plaque and in Dental Plaque Samples

Human oral, gut, and plaque microbiota in patients with atherosclerosis

Comprehensive Transcriptome Analysis of the Periodontopathogenic Bacterium Porphyromonas gingivalis W83

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