Detection of<i>H. pylori</i>DNA in gastric epithelial cells by<i>in situ</i>hybridization

Lu, Xinliang; Qian, Kan; Tang, Xun-qiu; Zhu, Yong-Liang; Du, Qin

doi:10.3748/wjg.v8.i2.305

Cited by 10 publications

(1 citation statement)

References 30 publications

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“…The sections were processed according to the standard protocol, 27 and digested with 10 g/ml protein K for 10 minutes at room temperature. Endogenous peroxidase was inactivated by incubating the tissue sections in 0.3% H 2 O 2 for 20 minutes.…”

Section: In Situ Hybridizationmentioning

confidence: 99%

ARK5 Expression in Colorectal Cancer and Its Implications for Tumor Progression

Kusakai

Suzuki

Ogura

et al. 2004

The American Journal of Pathology

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A novel member of the human AMPK family, ARK5, was recently discovered to be a key molecule in mediating cancer cell migration activity in human pancreas cancer cell line PANC-1, and its activation was found to be induced by Akt-dependent phosphorylation at Ser 600. DNA array analysis with 241 paired cDNAs from 13 different types of tumors and corresponding normal tissues derived from cancer patients revealed ARK5 overexpression in the samples of colorectal cancer. ARK5 expression was measured and an in vitro invasion assay was performed in six human colorectal cancer cell lines, WiDr, HCT-15, DLD-1, SW620, LoVo, and SW480, and since high invasion activity was concordant with higher ARK5 expression, ARK5 expression was examined in relation to tumor progression and metastatic activity in clinical samples. In 56 clinical samples of primary colorectal cancers and their liver metastases, higher ARK5 expression was observed in the samples from more advanced cases, and much higher expression was observed in the liver metastases. In situ hybridization analysis showed ARK5 overexpression in tumor cells. Based on these findings, we propose that ARK5 overexpression is involved in tumor progression of colon cancer clinically. Because of excessive proliferation, energy demands, and insufficient and structurally and functionally inappropriate angiogenesis, tumor tissues are often exposed to an insufficient blood supply, and in turn are usually exposed to both hypoxia and nutrient starvation in their microenvironment.1 The impact of the microenvironment on the progression of various tumors has been clearly recognized. The importance of the response of tumor cells to hypoxic conditions has long been studied, 2-4 and the angiogenic ability of tumors was first recognized as a key factor in tumor biology about 30 years ago by Folkman. 5 The molecular mechanism of tumor angiogenesis, and its control mechanisms have been thoroughly studied. 6,7 Excessive tumor angiogenesis is often naively regarded as improving tumor's blood supply, but tumor hypoxia is now known to be a good marker for poor prognosis in various cancers. 8 -10 When cells and tissues are exposed to hypoxia, they sustain their excessive growth and proliferation in this adverse environment by improving blood flow, cell cycle regulation, and energy metabolism.11,12 These reactions are called the hypoxic response, and hypoxia-inducible factor 1 (HIF-1) is known to be a key transcriptional factor in the response. HIF-1 transactivates a series of hypoxia response genes, including the genes encoding VEGF, erythropoietin, and glycolytic enzymes, in response to hypoxia, and for this reason the ability of tumor cells to produce angiogenic factors is often correlated with tumor's ability to invade, metastasize, and progress. 13-15Based on these observations, it has been proposed that exposure to hypoxia might stimulate the ability of tumor cells to produce angiogenic factors and in turn tumor stimulate invasion and metastasis by the tumor 16 -18 and that under these condit...

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Section: In Situ Hybridizationmentioning

confidence: 99%

ARK5 Expression in Colorectal Cancer and Its Implications for Tumor Progression

Kusakai

Suzuki

Ogura

et al. 2004

The American Journal of Pathology

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Establishment ofHelicobacter pyloriinfection model in Mongolian gerbils

Yan¹,

Luo²,

Mao³

2004

WJG

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AIM:To establish a stable and reliable model of Helicobacter pylori infection model in Mongolian gerbils and to observe pathological changes in gastric mucosa in infected animals. METHODS:Mongolian gerbils were randomly divided into 18 groups; 6 groups were infected with H pylori clinical strain Y06 (n=6, groups Y), 6 groups were infected with H pylori strain NCTC11637 (n=6, groups N), and 6 uninfected groups as negative controls (n=4, groups C). H pylori suspensions at the concentrations of 2×10 8 and 2×10 9 CFU/mL of strain NCTC11637 and strain Y06 were prepared. The animals in three groups N and in three groups Y were orally challenged once with 0.5 mL of the low concentration of the bacterial suspension. The animals in another three groups N and in another three groups Y were orally challenged with 0.5 mL of the high concentration of the bacterial suspension for 3 times at the intervals of 24 h, respectively. For the negative controls, the animals in six groups C were orally given w i th t he s am e v ol u m e o f Br u c el l a b r o t h a t t h e corresponding inoculating time. The animals were killed after 2nd, 4 th and 6 th week after the last challenge and the gastric mucosal specimens were taken for urease test, bacterial isolation, pathological and immunohistochemical examinations. RESULTS:Positive isolation rates of H pylori in the animals of groups Y at the 2nd, 4 th and 6 th week after one challenge were 0%, 16.7% and 66.7%, while in the animals of groups N were 0%, 0% and 16.7%, respectively. Positive isolation rates of H pylori in the animals of groups Y at the 2nd, 4 th and 6 th week after three challenges were 66.7%, 100% and 100%, while in the animals of groups N were 66.7%, 66.7% and 100%, respectively. In animals with positive isolation of H pylori, the bacterium was found to colonized on the surface of gastric mucosal cells and in the gastric pits, and the gastric mucosal lamina propria was infiltrated with inflammatory cells.

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Construction of prokaryotic expression system ofureBgene from a clinicalHelicobacter pyloristrain and identification of the recombinant protein immunity

Mao

Yan²

2004

WJG

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A prokaryotic expression system with high expression efficiency of H pylori ureB gene was successfully established. The expressed rUreB showed qualified immunoreactivity and antigenicity. High frequencies of UreB expression in different H pylori isolates and specific antibody against UreB in sera of H pylori infected patients indicate that UreB is an excellent antigen candidate for developing H pylori vaccine.

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Detection ofH. pyloriDNA in gastric epithelial cells byin situhybridization

Cited by 10 publications

References 30 publications

ARK5 Expression in Colorectal Cancer and Its Implications for Tumor Progression

ARK5 Expression in Colorectal Cancer and Its Implications for Tumor Progression

Establishment ofHelicobacter pyloriinfection model in Mongolian gerbils

Construction of prokaryotic expression system ofureBgene from a clinicalHelicobacter pyloristrain and identification of the recombinant protein immunity

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