Detection of <i>E. coli</i> Isolates in Water from Setumo Dam Mmabatho Area – North West Province, South Africa Using <i>mdh</i> Specific PCR Analysis

Dikobe, Tshegofatso Bridget; Sithebe, Nomathamsanqa Patricia; Ateba, Collins Njie

doi:10.1080/09709274.2011.11906414

Cited by 2 publications

(1 citation statement)

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“…Stock culture of a DEC 1, a DEC 2, a DEC 3, a DEC 4, and a DEC 5 was inoculated into Mac Conkey Agar plate media (Oxoid, UK) and incubated at 37°C for 24 hours. A positive result gives rise to a pink to rose red colonies, such as its ability to ferment lactose [ 27 , 28 ] ( Figure 1 ). Stock cultures of S. paratyphi A , S. paratyphi B, and S. flexneri isolates were inoculated into Salmonella Shigella Agar plate media (Oxoid, UK) and incubated at 37°C for 24 hours.…”

Section: Methodsmentioning

confidence: 99%

Isolation and Identification of Escherichia coli O157:H7 Lytic Bacteriophage from Environment Sewage

Sjahriani

Wasito

Tyasningsih

2021

International Journal of Food Science

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Escherichia coli O157:H7 is one of the pathogenic bacteria causing foodborne disease. The use of lytic bacteriophages can be a good solution to overcome the disease. This study is aimed at isolating lytic bacteriophages from environmental sewage with E. coli O157:H7 bacterial cells. The sample used in this study was eight bacteriophages, and the technique used in identifying E. coli O157:H7 carriers of the stx1 and stx2 genes was PCR. The double layer plaque technique was used to classify bacteriophages. Plaque morphology, host specificity, and electron micrograph were used to identify the bacteriophages. The result obtained plaque morphology as a clear zone with the largest diameter size of 3.5 mm. Lytic bacteriophage could infect E. coli O157:H7 at the highest titer of 10 × 10 8 PFU / mL . Bacteriophages have been identified as Siphoviridae and Myoviridae. Phage 3, phage 4, and phage 8 could infect Atypical Diarrheagenic E. coli 1 (aDEC1) due to their host specificity. The Friedman statistical tests indicate that lytic bacteriophage can significantly lyse E. coli O157:H7 ( p = 0.012 ). The lysis of E. coli O157:H7 by phage 1, phage 2, phage 3, and phage 5 bacteriophages was statistically significant, according to Conover’s posthoc test ( p < 0.05 ). The conclusion obtained from this study is that lytic bacteriophages from environmental sewage could lyse E. coli O157:H7. Therefore, it could be an alternative biocontrol agent against E. coli O157:H7 that contaminates food causing foodborne disease.

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Section: Methodsmentioning

confidence: 99%