Detection of
            <i>Chlamydia pneumoniae</i>
            DNA in CD3+ Lymphocytes From Healthy Blood Donors and Patients With Coronary Artery Disease

Kaul, Ravi; Uphoff, Janet; Wiedeman, Jean A.; Yadlapalli, Sanjay; Wenman, Wanda M.

doi:10.1161/01.cir.102.19.2341

Cited by 68 publications

(52 citation statements)

References 21 publications

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“…The pathogens enter the bloodstream via monocytes and migrate into the vessel wall into already existing plaques by leukocyte infiltration (1,32). Recently, the presence of C. pneumoniae DNA in circulating PBMCs has been proposed as a marker for chronic C. pneumoniae infection and several results have been reported, but that frequency varies (4,5,7,16,18,34,37). In this study, we evaluated the newly designed 53 kDa protein gene nested PCR using highly purified EBs and PBMCs from healthy blood donors and compared it with four different PCR assays which were recommended by the CDC and the LCDC.…”

Section: Discussionmentioning

confidence: 99%

Comparison of Five PCR Assays for Detecting Chlamydophila pneumoniae DNA

Fukano

2004

Microbiology and Immunology

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We compared five different polymerase chain reaction (PCR) assays for the detection of Chlamydophila pneumoniae DNA using highly purified elementary bodies (EBs) and peripheral blood mononuclear cells (PBMCs) from healthy blood donors. The primers were as follows; two targeting the 16S rRNA gene, one targeting the ompA gene, one targeting the Pst‐I gene, and one targeting the 53 kDa outer membrane protein gene. The 16S rRNA touchdown enzyme time release (TETR) PCR, the ompA nested PCR and the 53 kDa nested PCR were the most sensitive assays and could detect one or more EB per assay. These three PCRs also had the same reproducibility, but the minimal amount of C. pneumoniae that could be reproducibly detected (10 of 10 testing positive) was 20 EBs. In a sample of specimens from healthy blood donors, we found 5 of 77 (6.5%) PBMCs specimens to have C. pneumoniae DNA according to the nested ompA PCR. Specimens with the 16S rRNA TETR and 53 kDa nested assays were found to have C. pneumoniae DNA 7 of 77 (9.1%) and 18 of 77 (23.4%) specimens, respectively. The other two assays failed to detect even a single positive. However, the detection rate decreased with repeated testing of the same samples. Our newly designed 53 kDa nested PCR may be as useful as the other four recommended PCR assays and may be a more useful assay for the detection of C. pneumoniae DNA from PBMCs.

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Section: Discussionmentioning

confidence: 99%

Comparison of Five PCR Assays for Detecting Chlamydophila pneumoniae DNA

Fukano

2004

Microbiology and Immunology

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“…Recently, our studies demonstrated that C. pneumoniae can infect and multiply in the human lymphocyte cell line Molt-4 and mouse spleen lymphocytes [14,15]. Furthermore, Kaul et al reported C. pneumoniae DNA in peripheral blood leukocytes obtained from cardiology patients [16]. Thus, lymphocytes are a potential host cell for C. pneumoniae infection.…”

Section: Introductionmentioning

confidence: 82%

Chlamydophila pneumoniae attachment and infection in low proteoglycan expressing human lymphoid Jurkat cells

Kobayashi

Ishida

Matsuo

et al. 2011

Microbial Pathogenesis

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This study investigated the proteoglycan (PG)-dependent mechanism ofImmunofluorescent co-staining with antibodies against chlamydial LPS and heparin did not identify bacterial and heparin co-localization on Jurkat cells. We also confirmed that when C. pneumoniae was statically infected to human CD4 + peripheral blood lymphocytes known not expressing detectable level of heparin, the bacteria attached to and formed inclusion bodies in the cells. Thus, the attachment mechanism of C.pneumoniae to Jurkat cells with low PG expression is unique when compared withHEp-2 cells and potentially independent of GAGs such as heparin.

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“…C. pneumoniae presence was confirmed in PBMCs, including monocytes and lymphocytes, by a PCR technique [20,37]. The pathogen presence in PBMCs may indicate a current infection or it may be harbored within these cells in a persistent state [37][38][39]. More than 20 studies have investigated the prevalence of C. pneumoniae DNA in PBMCs in cardiovascular patients and controls from different populations.…”

Section: Discussionmentioning

confidence: 99%

Lack of strong association of Chlamydia pneumoniae and atherosclerosis in a Jordanian population

Al-Younes

Abeeleh

Jaber

2016

J Infect Dev Ctries

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Introduction: The correlation of Chlamydia pneumoniae to coronary artery disease (CAD) in Jordan was investigated in this study. Methodology: Totals of 361 atherosclerotic patients and 392 apparently healthy controls of both sexes were enrolled. C. pneumoniae-specific IgG antibodies were measured by the microimmunofluorescence assay (MIF). The presence of the bacterial DNA in the blood by polymerase chain reaction (PCR) was also tested. Results: The overall IgG seroprevalence, estimated at a titer of 1/16, was insignificantly higher in patients (75.9%) than in controls (71.7%). About 59.3% of patients demonstrated seropositivity at titers ≤ 1/256, which are suggestive of chronic or presumed past infection, whereas 54.1% of controls were seropositive at these titers (p > 0.05). Analysis of gender-specific seroprevalences revealed no obvious relation between C. pneumoniae and atherosclerosis in males (78.9% and 77.9% in atherosclerotic and control males, respectively; p > 0.05). However, a significantly elevated seropositivity was detected in atherosclerotic females (71.7%) compared with control females (64.2%). On the other hand, the PCR-based detection of C. pneumoniae DNA failed to correlate the bacterium to atherosclerosis. Conclusions: We were unable to show a strong association between C. pneumoniae and CAD, potentially because of the presence of high seroprevalence of C. pneumoniae antibodies and the unreliability of the whole blood-based nested PCR technique used.

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Detection of Chlamydia pneumoniae DNA in CD3+ Lymphocytes From Healthy Blood Donors and Patients With Coronary Artery Disease

Cited by 68 publications

References 21 publications

Comparison of Five PCR Assays for Detecting Chlamydophila pneumoniae DNA

Comparison of Five PCR Assays for Detecting Chlamydophila pneumoniae DNA

Chlamydophila pneumoniae attachment and infection in low proteoglycan expressing human lymphoid Jurkat cells

Lack of strong association of Chlamydia pneumoniae and atherosclerosis in a Jordanian population

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