Detection of Campylobacter and Shigella Species in Food Samples Using an Array Biosensor

Abstract

Campylobacter and Shigella bacteria are common causes of food- and water-borne illness worldwide. There is a current need in food, medical, environmental, and military markets for a rapid and user-friendly method of detecting such pathogens. The array biosensor developed at the NRL encompasses these qualities. In this study, 25-min, sandwich immunoassays were developed for the detection of Campylobacter and Shigella species in both buffer and a variety of food and beverage samples. The limit of detection for Shigella dysenteriae in buffer and chicken carcass wash was 4.9 x 10(4) cfu mL(-)(1), whereas Campylobacter jejuni could be measured at concentrations as low as 9.7 x 10(2) cfu mL(-)(1). The limits of detection and dynamic range were found to vary depending on the sample matrix, but could be improved by running the sample over the waveguide surface for longer periods of time. Samples were run with no preconcentration or enrichment steps and little-to-no sample pretreatment prior to analysis.

The Array Biosensor: Portable, Automated Systems

“…14,[16][17][18][19][20][21][22][23][24][25][26][27][28][29][30][31][32] Table 1 provides detailed information on the assays including limits of detection (LOD). The LOD was calculated as the lowest concentration tested which gave a signal 3 standard deviations above the blank.…”

“…33 Due to public health risks, much of the recent work in assay development has concentrated on the rapid detection of foodborne contaminants. The NRL Array Biosensor has been used for the measurement of a number of protein toxins, such as staphylococcal enterotoxin B (SEB) 22,23 and botulinum toxoid A, 22 and bacteria, including Campylobacter spp., 29 Shigella spp., 29 Escherichia coli 32 and Salmonella enterica serovar Typhimurium, 28 in a variety of spiked food matrices. Analyte detection is rapid, with immunoassays taking 10 -45 min, is simple to perform and requires minimal sample processing.…”

Genotypic Characterization of <i>Shigella</i> Species Isolated from Abattoirs in the North West Province, South Africa Using PCR Analysis

“…Several PCR protocols using different molecular markers have been used for the detection of Shigella species in environmental, faecal, and food samples [15][16][17][18][19][20][21][22]. The invasion plasmid antigen H (IpaH) and the IpaABCD genes are virulence gene markers whose protein products are necessary for invasion of colonic epithelial cells.…”

Biosensor Arrays for Environmental Monitoring

“…The array biosensor developed at the NRL encompasses these qualities. Ligler et al [30 ] reported on a sandwich immunoassaybased biosensor array that was developed for the detection of Campylobacter and Shigella species in both buffer and a variety of food and beverage samples. The limit of detection for Shigella dysenteriae in buffer and chicken carcass wash was 4.9×10 4 CFU/mL, whereas Campylobacter jejuni could be measured at concentrations as low as 9.7×10 2 CFU/mL.…”