Detection of Aequorea victoria Green Fluorescent Protein by Capillary Electrophoresis Laser Induced Fluorescence Detection

Using capillary electrophoresis with laser-induced fluorescence to study the interaction of green fluorescent protein-labeled calmodulin with Ca2+- and calmodulin-binding protein

“…Another advantage of GFP is that its excitation spectrum within the visible range has an excitation line compatible with the frequently used argon ion laser, so it could be used as a fluorescent probe in CE. Analysis of GFP by CE-LIF has been reported [21][22][23][24].…”

“…4). As previously reported, GFP was previously quantified by capillary electrophoresis [23], and the limit-of-detection (LOD) was 3.0 × 10 −12 M for an injection volume of 17 nl. In our work, LODs of both GFP and GFP fused protein (GFP-CaM) were also studied by serial dilution of the samples until the analyte peak was no longer visible above the baseline noise (Fig.…”

“…Thus it is essential for an ideal system in which components are detected in their native form without a chemical tagging process that may disturb the cellular environments. Recently a group of natively fluorescent proteins have been applied in molecular and cellular studies [16][17][18][19][20] and can be detected by CE-LIF [21][22][23][24]. One of them, green fluorescent protein (GFP) could be used for this purpose.…”

Green fluorescent protein inSaccharomyces cerevisiae: Real-time studies of theGAL1 promoter

“…Other than E. coli, GFP has also been used to monitor protein production in insect cells (Cha et al, 1997). Meanwhile, various methods and devices for quantitative measurements of GFP were developed (Randers-Eichhorn et al, 1997;Craig et al, 1997;Korf et al, 1997;Endow and Piston, 1998;Knight et al, 1999).…”

Combination of On-Chip Field Amplification and Bovine Serum Albumin Sweeping for Ultrasensitive Detection of Green Fluorescent Protein

“…For the sensitive detection of proteins, conventional capillary electrophoresis often offers limits of detection at nanomolar levels33 with laser-induced fluorescence (LIF) detection, although lower limits of detection have been reported 34,35. Limits of detection of similar scales have also been obtained on microchips 36-39.…”