Detection of hypothetical proteins in 10 individual human tumor cell lines

Afjehi‐Sadat, Leila; Shin, Joo‐Ho; Felizardo, Maureen; Lee, Kiseok; Slavc, Irene; Lubec, Gert

doi:10.1016/j.bbapap.2004.09.024

Cited by 12 publications

(15 citation statements)

References 48 publications

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“…This could be delineated by the higher number of polyphenol compounds present in both the aqueous (9 out of 10 polyphenols) and methanolic (3 out of 4 polyphenols) P. urinaria extracts [17,20], hence having a higher capability to cause antimetastatic activities on A549. Some of those proteins were represented by more than one spot, which represents different splicing forms of the same protein as a result of post-translational modification [14]. …”

Section: Discussionmentioning

confidence: 99%

“…Similar to other malignant pathologies, tumor markers for NSCLC remained inadequate. Serum biomarkers employed in the current clinical setting such as ENO (enolase alpha), CEA (carcinoembryonic antigen), SCC (squamous cell carcinoma), CA-125 (cancer antigen 125) or TPA (tissue polypeptide antigen) are not satisfactory due to their low sensitivity and specificity [13,14]. In the quest for novel diagnostic or prognostic biomarkers, the proteomic technique is ideal since it permits qualitative and quantitative analysis of numerous proteins simultaneously [9,13].…”

Section: Introductionmentioning

confidence: 99%

“…In the quest for novel diagnostic or prognostic biomarkers, the proteomic technique is ideal since it permits qualitative and quantitative analysis of numerous proteins simultaneously [9,13]. Moreover, the search for biomarkers at the protein level is more dependable than at the transcriptional level as protein expression may not necessarily associate with mRNA expression [14]. …”

Section: Introductionmentioning

confidence: 99%

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Inhibition of Raf-MEK-ERK and Hypoxia pathways by Phyllanthus prevents metastasis in human lung (A549) cancer cell line

Lee

Jaganath

Manikam

et al. 2013

BMC Complement Altern Med

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BackgroundLung cancer constitutes one of the malignancies with the greatest incidence and mortality rates with 1.6 million new cases and 1.4 million deaths each year. Prognosis remains poor due to deleterious development of multidrug resistance resulting in less than 15% lung cancer patients reaching five years survival. We have previously shown that Phyllanthus induced apoptosis in conjunction with its antimetastastic action. In the current study, we aimed to determine the signaling pathways utilized by Phyllanthus to exert its antimetastatic activities.MethodsCancer 10-pathway reporter array was performed to screen the pathways affected by Phyllanthus in lung carcinoma cell line (A549) to exert its antimetastatic effects. Results from this array were then confirmed with western blotting, cell cycle analysis, zymography technique, and cell based ELISA assay for human total iNOS. Two-dimensional gel electrophoresis was subsequently carried out to study the differential protein expressions in A549 after treatment with Phyllanthus.ResultsPhyllanthus was observed to cause antimetastatic activities by inhibiting ERK1/2 pathway via suppression of Raf protein. Inhibition of this pathway resulted in the suppression of MMP2, MMP7, and MMP9 expression to stop A549 metastasis. Phyllanthus also inhibits hypoxia pathway via inhibition of HIF-1α that led to reduced VEGF and iNOS expressions. Proteomic analysis revealed a number of proteins downregulated by Phyllanthus that were involved in metastatic processes, including invasion and mobility proteins (cytoskeletal proteins), transcriptional proteins (proliferating cell nuclear antigen; zinc finger protein), antiapoptotic protein (Bcl2) and various glycolytic enzymes. Among the four Phyllanthus species tested, P. urinaria showed the greatest antimetastatic activity.ConclusionsPhyllanthus inhibits A549 metastasis by suppressing ERK1/2 and hypoxia pathways that led to suppression of various critical proteins for A549 invasion and migration.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Inhibition of Raf-MEK-ERK and Hypoxia pathways by Phyllanthus prevents metastasis in human lung (A549) cancer cell line

Lee

Jaganath

Manikam

et al. 2013

BMC Complement Altern Med

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“…The most commonly used comparative proteomic approach is two-dimensional difference gel electrophoresis (2D-DIGE) coupled with mass spectrometry (MS), which provides a good proteomic tool for the investigation of novel proteins that might serve as candidates for tumor markers. Previously, 2D-PAGE analyses of protein changes were performed to study chemoresistance, 22 to search for markers for tumor diagnosis in NB cell lines, 23,24 and to study quantitatively and qualitatively differences in healthy and pathological NB mouse samples. 25,26 Moreover, proteomic characterization of differentiation induced by ATRA has been described in promyelocytic cells 7,27,28 and in mouse embryonic stem cells.…”

Section: Introductionmentioning

confidence: 99%

Comparative Proteomic Expression Profile in All-trans Retinoic Acid Differentiated Neuroblastoma Cell Line

et al. 2007

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Neuroblastoma (NB) is an infant tumor which frequently differentiates into neurons. We used twodimensional differential in-gel electrophoresis (2D-DIGE) to analyze the cytosolic and nuclear protein expression patterns of LAN-5 cells following neuronal differentiating agent all-trans-retinoic acid treatment. We identified several candidate proteins, from which G 2 and Prefoldin 3 may have a role on NB development. These results strength the use of proteomics to discover new putative protein targets in cancer.

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“…The search for markers and vaccines at the protein level is more reliable than at the transcriptional level, as the translation process is often unpredictable and the spatiotemporal in situ localization of a given protein is not often correlated with its in situ expression pattern [8] . Although several genomic studies have been performed using pediatric OS primary tumor tissues or cells [9,10] , comparative proteomic studies on OS are rare.…”

Section: Introductionmentioning

confidence: 99%

Comparative proteomic analysis of human osteosarcoma and SV40-immortalized normal osteoblastic cell lines

Guo

Shen

Jin

et al. 2007

Acta Pharmacologica Sinica

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Aim: Comparative proteomics provide a powerful approach in screening for alterations in protein levels and post‐translational modifications that are associated with tumors. In the present study, we aimed to identify candidate biomarkers to distinguish osteosarcoma (OS) cells from normal osteoblastic cells. Methods: We employed 3 OS cell lines (U2OS, IOR/OS9, and SaOS‐2), and used the SV40‐immortalized normal osteoblastic cell line (hFOB1.19) as the control. The differential protein levels in OS and osteoblastic cells were identified using 2‐D gel electrophoresis followed by matrix‐assisted laser desorption/ionization‐time of flight mass spectrometry analyses. Two proteins of interest, the levels of which were significantly increased in OS cells, were further characterized by Western blot analyses. Results: Twenty‐six proteins were identified, the expression level of which was either significantly increased or decreased in the OS cells as compared to the control cells. The expression level of the activator of 90 kDa shock protein ATPase homolog 1 (AHA1), was enhanced 12.4‐, 24.1‐, and 23.8‐fold in SaOS‐2, IOR/OS9, and U2OS cells, respectively, and the level of the stomatin‐like protein 2 (SLP‐2) was increased by 10.4‐ and 7.8‐fold in IOR/OS9 and U2OS cells, respectively, as compared to normal osteoblastic cells. Those observations were confirmed by Western blot analyses. Conclusion: A differential proteomic analysis was successfully used to identify AHA1 and SLP‐2 that were significantly overproduced in OS cells as compared to normal osteoblastic cells, suggesting that those proteins among others may be effective biomarker candidates for the identification of OS cells.

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Detection of hypothetical proteins in 10 individual human tumor cell lines

Cited by 12 publications

References 48 publications

Inhibition of Raf-MEK-ERK and Hypoxia pathways by Phyllanthus prevents metastasis in human lung (A549) cancer cell line

Inhibition of Raf-MEK-ERK and Hypoxia pathways by Phyllanthus prevents metastasis in human lung (A549) cancer cell line

Comparative Proteomic Expression Profile in All-trans Retinoic Acid Differentiated Neuroblastoma Cell Line

Comparative proteomic analysis of human osteosarcoma and SV40-immortalized normal osteoblastic cell lines

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