Detection of humoral and cell-mediated immunity to kidney basement membranes in human renal diseases

New avian model of experimental glomerulonephritis consistent with mediation by cellular immunity. Nonhumorally mediated glomerulonephritis in chickens.

“…Increasing evidence, however, indicates an important role for CMI in the pathogenesis ofmany human and experimental animal types of nephritis. The macrophage appears to play a critical part in the proliferation and GBM damage in certain forms of nephritis (4)(5)(6)(7)(8)(9)(10)(11)(12)(13)(14)(15)(16); lymphocytes may participate in yet other mechanisms of immunologic damage (17,18); and CMI of uncertain type may be important in still other forms of nephritis (19)(20)(21)(22)(23)(24)(25)(26)(27).…”

Carbohydrate and Amino‐Acid Composition of Human Glomerular‐Basement‐Membrane Fractions Purified by Affinity Chromatography

“…Using affinity chromatography, membrane components have been purified ; they contain both types of oligosaccharidic chains and react, with rabbit anti-human GBM IgG, by passive hemagglutination [22]. Moreover, thesc labelled fractions can be used to detect, by radioimmunoassay, anti-GEM antibodies in the sera or kidney eluates of patients with Goodpasture's syndrome [27].…”

“…The passive hemagglutination test for the detection of anti-GRM antibodies was performed after coating glomerular-basement-membrane antigens on sheep red blood cells with chromium chloride [22].…”

“…Both fractions were then dialyzed against 0.05 M ammonium bicarbonate and lyophilized. They were tested by passive hemagglutination with anti-GBM IgG [22] Labelling of Solubilized Glomerular Basement Membranes with Tritium The radioactivity of tritiated membranes was 6 counts x min-l x ng protein-I. A control preparation which was not tritiated by the sodium periodate had 0.5 counts x min-1 x ng protein-I, after otherwise identical treatment.…”

“…to Anti-GBM IgC Rabbit anti-GBM IgG had a hemagglutinating titer of 1/104 [22] ; 70°/, of the IgG was bound on the Sepharose cyanogen-bromide (percentage calculated by the protein content [21] of the wash after coating). 80 mg of labelled membrane glycoprotcins were submitted to the affinity chromatography.…”