Detection of human papillomavirus in cholesteatomas

Chao, Wai-Nang; Chang, Shang‐Jen; Jin, Yin Tai

doi:10.1007/s004050050206

Cited by 11 publications

(14 citation statements)

References 17 publications

(24 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The present study and the earlier results of Chao et al [11] both show a low prevalence of HPV DNA in cholesteatomas. Based on these results, an aetiological role of HPVs in the pathogenesis of cholesteatomas is unlikely, even if the histopathological appearance of a cholesteatoma exhibits features of HPV-induced lesions.…”

Section: Discussionsupporting

confidence: 62%

See 1 more Smart Citation

Prevalence of Human Papillomavirus DNA in Cholesteatomas

Franz

Teschendorf²,

Wohlschläger³

et al. 2007

ORL

View full text Add to dashboard Cite

Cholesteatomas show histomorphological features like papillary growth and koilocytosis, which are characteristic of lesions induced by human papillomaviruses (HPV). Two previous studies investigating the possible role of HPV in the development of cholesteatoma had detected HPV-6 and HPV-11 DNA with a prevalence differing from 3 to 36%. The aim of the presented study was to evaluate the prevalence of different HPV types in cholesteatomas using a sensitive detection system for HPV DNA. Twenty-nine biopsies from cholesteatomas were screened for HPV DNA with a 2-step broad-spectrum PCR (PCR and nested PCR). HPV-positive products were directly sequenced by means of a cycle sequencing approach. Sensitivity of the applied broad-spectrum PCR was 0.1 copy/genome. One out of 29 biopsies showed a positive signal on the nested PCR level. Considering the low prevalence (1/29 biopsies) of detected HPV DNA in cholesteatomas, infections with common HPV types are unlikely to be a causative factor.

show abstract

Section: Discussionsupporting

confidence: 62%

“…Based on the results of these two studies, Chao et al [11] focused on the detection of HPV-6 and -11 DNA in cholesteatomas. They applied PCR and in situ hybridization with an HPV-6-or HPV-11-specific probe, claiming a sensitivity of 0.1 viral copy/cell.…”

Section: Discussionmentioning

confidence: 99%

Prevalence of Human Papillomavirus DNA in Cholesteatomas

Franz

Teschendorf²,

Wohlschläger³

et al. 2007

ORL

View full text Add to dashboard Cite

show abstract

“…Ectopic migration of ectodermal tissue to the middle ear may also be implied in the genesis of this pathology 4,5 . Chao et al 6 showed association between HPV and cholesteatoma and middle ear papilloma without defining a causal correlation. However, Xia et al 1 demonstrated a dissemination of the squamous papilloma of nasopharynx to the tympanic membrane through the performance of myringotomy concomitantly with papilloma surgical treatment in the nasopharynx, indicating the likely infectious nature of the lesion.…”

Section: Discussionmentioning

confidence: 99%

Papiloma escamoso de orelha média: relato de um caso e revisão da literatura

Cahali

Silva²,

Machado³

et al. 2005

Rev. Bras. Otorrinolaringol.

View full text Add to dashboard Cite

show abstract

“…There is great interest in the etiopathogenesis of HPV-associated cholesteatoma because HPV commonly infects the stratified epithelium [17,18]. However, conflicting data have been reported for HPV in COMC [10,[19][20][21]. HPV sequences have been detected in COMC with different prevalence, ranging from 3% to 70% [10,[19][20][21].…”

mentioning

confidence: 99%

“…However, conflicting data have been reported for HPV in COMC [10,[19][20][21]. HPV sequences have been detected in COMC with different prevalence, ranging from 3% to 70% [10,[19][20][21]. Moreover, no specific HPV genotypes have been associated with COMC, as high-and low-risk HPVs, such as HPV16, HPV18 and HPV6 and HPV11, have been detected [10,[19][20][21].There is emerging evidence that HPV infection can occur in different anatomical sites.…”

mentioning

confidence: 99%

High Human Papillomavirus DNA loads in Inflammatory Middle Ear Diseases

et al. 2020

View full text Add to dashboard Cite

Background. Previous studies reported human papillomaviruses (HPVs) in middle ear tumors, whereas these viruses have been poorly investigated in chronic inflammatory middle ear diseases. We investigated HPVs in non-tumor middle ear diseases, including chronic otitis media (COM). Methods. COM specimens (n = 52), including chronic suppurative otitis media (CSOM) (n =38) and cholesteatoma (COMC) (n = 14), as well as normal middle ear (NME) specimens (n = 56) were analyzed. HPV sequences and DNA loads were analyzed by quantitative-PCR. HPV genotyping was performed by direct sequencing. Results. HPV DNA was detected in 23% (12/52) of COM and in 30.4% (17/56) of NME (p > 0.05). Specifically, HPV DNA sequences were found in 26.3% (10/38) of CSOM and in 14.3% (2/14) of COMC (p > 0.05). Interestingly, the HPV DNA load was higher in COMC (mean 7.47 copy/cell) than in CSOM (mean 1.02 copy/cell) and NME (mean 1.18 copy/cell) (P = 0.03 and P = 0.017 versus CSOM and NME, respectively). HPV16 and HPV18 were the main genotypes detected in COMC, CSOM and NME. Conclusions. These data suggest that HPV may infect the middle ear mucosa, whereas HPV-positive COMCs are associated with higher viral DNA loads as compared to NME.Pathogens 2020, 9, 224 2 of 10 mucosa sampled from CSOM patients [11]. However, the etiology of CSOM remains to be determined. The relationship between HPV infection and inflammation has been previously reported [12]. It has been shown that persistent infection with high-risk HPVs leads to an increase in pro-inflammatory cytokines, including IL-6, TNF-α and MIP-1α [13]. In addition, high-risk HPV type 16 (HPV16) is able to increase the expression of cyclooxygenase-2 (COX-2), a key enzyme in the synthesis of prostaglandins, which are important mediators of inflammation [14,15]. Until now, only a single study has reported HPV DNA sequences in CSOM, whereby different HPV genotypes, including HPV16, HPV18 and HPV6, have been detected in 30.7% of CSOM [4].COMC is a form of expanding growth consisting of keratinizing squamous cell epithelium [16]. There is great interest in the etiopathogenesis of HPV-associated cholesteatoma because HPV commonly infects the stratified epithelium [17,18]. However, conflicting data have been reported for HPV in COMC [10,[19][20][21]. HPV sequences have been detected in COMC with different prevalence, ranging from 3% to 70% [10,[19][20][21]. Moreover, no specific HPV genotypes have been associated with COMC, as high-and low-risk HPVs, such as HPV16, HPV18 and HPV6 and HPV11, have been detected [10,[19][20][21].There is emerging evidence that HPV infection can occur in different anatomical sites. Since HPV infects epithelia [22], all anatomical sites covered with epithelial tissue are potentially exposed to HPV infection. Apart from pluristratified tissues of the cervix [23], vulva [24] and oral pharynx [25], HPV sequences have been detected in simple epithelia from several anatomical sites such as lung [26], upper respiratory tract [27], larynx [28] and nose [29]. Since the middle ear muc...

show abstract

Detection of human papillomavirus in cholesteatomas

Cited by 11 publications

References 17 publications

Prevalence of Human Papillomavirus DNA in Cholesteatomas

Prevalence of Human Papillomavirus DNA in Cholesteatomas

Papiloma escamoso de orelha média: relato de um caso e revisão da literatura

High Human Papillomavirus DNA loads in Inflammatory Middle Ear Diseases

Contact Info

Product

Resources

About